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Showing papers on "Respiratory epithelium published in 1972"


Journal ArticleDOI
TL;DR: Organ cultures of bovine tracheal epithelium were infected with a rhinovirus or a strain of parainfluenza type 3 virus, and the epithelial surfaces were studied by scanning electron microscopy.
Abstract: Organ cultures of bovine tracheal epithelium were infected with a rhinovirus or a strain of parainfluenza type 3 virus, and the epithelial surfaces were studied by scanning electron microscopy. When washed free from mucus, normal control cultures showed a thick carpet of normal cilia, whereas the two viruses each produced specific morphological abnormalities. In rhinovirus-infected cultures, degenerating ciliated and nonciliated cells with finely granular surfaces were rapidly extruded from the epithelium. The denuded epithelial surface was relatively smooth, and showed some evidence of squamous metaplasia. By contrast, in cultures infected with parainfluenza type 3 virus, damage developed more slowly and the epithelial surface was ultimately covered with a profuse array of short microvillous projections. In thin sections, some of these were shown to be the sites of viral maturation.

38 citations



Journal ArticleDOI
01 Jan 1972
TL;DR: The mucosa in the lower oesophagus lined by columnar epithelium was very similar to the mucosa lining the cardia of the stomach, and it is supposed that such esophageal epithelia represents an upgrowth of cardia mucosa as a response to injury of the squamous epithelio of the oesphagus caused by chronic reflux of gastric contents.
Abstract: Biopsies of five patients with Barrett's syndrome were examined by light microscopic, cytochemical and electron microscopic techniques. It was shown that the mucosa in the lower oesophagus lined by columnar epithelium was very similar to the mucosa lining the cardia of the stomach. It is supposed that such esophageal epithelium represents an upgrowth of cardia mucosa as a response to injury of the squamous epithelium of the oesophagus caused by chronic reflux of gastric contents. The occurrence of goblet cells and patches of villous columnar cells was interpreted as intestinal metaplasia.

24 citations


Journal ArticleDOI
TL;DR: In this article, a chicken embryo tracheal organ cultures supported the growth of influenza A2 (Hong Kong) virus, and the entry of the influenza virus into, and its release from differentiated cells of the respiratory epithelium was followed with the aid of electron microscopy.
Abstract: Chick embryo tracheal organ cultures supported the growth of influenza A2 (Hong Kong) virus. Entry of the influenza virus into, and its release from, differentiated cells of the respiratory epithelium was followed with the aid of electron microscopy. Sections prepared from organ cultures revealed the presence of a mucous layer covering the epithelium, and passage of the virus through this mucus was found to be greatly enhanced at higher than 4° C temperatures.

24 citations


Journal ArticleDOI
TL;DR: Rats were exposed to the smoke of non-filter cigarettes for various periods of time, and they revealed signs of irritating smoke inhalation.
Abstract: Rats were exposed to the smoke of non-filter cigarettes for various periods of time. The weight gain of the exposed animals was markedly less than that of controls, and they revealed signs of irritati

14 citations




Journal ArticleDOI
K. Ogawa1
TL;DR: The structural organization of the epithelium at the middle part of human anal sinus was studied with electron microscope and found that differentiated cells contain a variable number of secretory granules in the supranuclear portion, but basal cells and some of cells in the surface layer do not have such granules.
Abstract: The structural organization of the epithelium at the middle part of human anal sinus was studied with electron microscope. The observed area is the portion covered by the stratified squamouns epithelium, stratified cuboidal or columnar epithelium, simple cuboidal epithelium and pseudostratified columnar epithelium.In the region covered by stratified squanous epithelium, the epithelial cells are similar to the cells of the transitional skin in human anal canal, but its cytoplasm contain well developed organelles. Especially glycogen granules and Odland bodies appear in the cells of granular layer and the upper part of spinous layer. In granular layer, two kinds of keratohyalin granules, round and irregular, are observed. In the surface layer, keratinization does not always progress uniformly.In the regions devoid of stratified squamous epithelium, most of differentiated cells contain a variable number of secretory granules in the supranuclear portion, but basal cells and some of cells in the surface layer do not have such granules. Their granules are considered to be produced from Golgi apparatus and they contain PAS-positive material. Of free surface of the cells in these area, there are found a few irregula short microvilli. They are coveredd filamentous or particular surface coat.Endocrine cells are generally columar in shape and are scattered among the other epithelial cells. These are usually in direct contact with the basement membrane and the apex of cells almost do not reach to the free border. Endocrine cells appear in the region devoid of stratified squamous epithelium and they have no relation to the crypt or gland. Abundant round granules with variable electron density, measuring about 130-150 mμ in dia-meter, are located in the infranuclear portion of the cell.Wandering cells are found among the epithelial cells in the observed area.