Resveratrol

About: Resveratrol is a research topic. Over the lifetime, 9348 publications have been published within this topic receiving 393838 citations. The topic is also known as: 3,4',5-stilbenetriol & 3,5,4'-trihydroxystilbene.

Effect of Resveratrol on Dipeptidyl Peptidase-4 (DPP-4) and Phospho Enol Pyruvate Carboxy Kinase (PEPCK) in Streptozotocin -Induced Diabetic Rats

Abstract: The aim of the present study was to determine the effect of resveratrol on dipeptidyl peptidase 4 (DPP-4) and phosphoenolpyruvate carboxykinase (PEPCK) enzyme activities in streptozotocin (STZ) induced diabetic rats, trying to find an explanation for its hypoglycemic effect. Sixty male Wistar Albino rats were included in the present study and classified into three groups, group I (control,non-diabetic), group II (diabetic group),group ІІІ (diabetic rats treated with resveratrol, 5 mg/kg body weight/day) for 30 days. After 30 days, blood was collected for determination of; serum glucose, insulin and DPP-4 enzyme activity. The liver was excised for estimation of liver glycogen and PEPCK enzyme activity. Serum glucose, DPP-4 activity, and liver PEPCK activity were significantly increased but serum insulin and liver glycogen were significantly decreased in STZ treated group, while upon resveratrol treatment , they were all reversed with no significant difference between group I and group III ( except for final fasting serum glucose level). Conclusion the antihyperglycemic effect of resveratrol may be related to its stimulatory effect on insulin ,its suppressive effect on :either DPP-4, so increases the level of incretins with subsequent increase in insulin release followed by lowering blood glucose level ,or its inhibitory effect on PEPCK enzyme activity and subsequent decrease in gluconeogenesis, or, finally its stimulation of glucose utilization by increasing glycogen formation.

The protective effect of resveratrol on brain injury in rats with severe acute pancreatitis

Abstract: Objective To explore the protective effect of resveratrol on rat brain injury resulting from severe acute pancreatitis (SAP). Methods Ninety-six male Sprague-Dawley rats were randomly divided into four groups:sham-operation (SO) group,severe acute pancreatitis (SAP) group,resveratrol-treated (RES) group and dexamethasone-treated (DEX) group,with eight rats in each group evaluated at 3,6 and 12 h. Levels of serum myelin basic protein (MBP),tight junction protein zonula occludens 1 (Zo-1),TNF-α and IL-6 were determined by ELISA. The ultrastructural changes of the brain and pancreatic tissues were examined using a transmission electron microscope. Results MBP,Zo-1,TNF-α and IL-6 levels in RES group were lower than those in SAP group at all time points (P0.05). RES and DEX groups had a significantly improved brain pathology compared to SAP group,which had ultrastructural changes such as obvious neuron swelling,capillary hemostasis,thrombosis and cell apoptosis. The parameters did not differ significantly between RES and DEX groups (P0.05). Conclusion The degradation of Zo-1 is involved in the pathophysiology of brain injury in SAP; MBP can be used as a marker of brain injury in SAP rats. Resveratrol can inhibit brain injury associated with SAP.

Resveratrol derivative that generates hydrogen and exhibits keratin producing action and method for producing the derivative

Abstract: PROBLEM TO BE SOLVED: To provide a resveratrol derivative that generates hydrogen and exhibits keratin producing action, which is useful as a cosmetic, a food preparation, and a medicine, and which is, especially when used in cosmetic, expected to improve degradation of sebum, moisture holding power of skin, and resilience of skin, and to provide a method for producing the derivative.SOLUTION: Provided is a resveratrol derivative having a structure represented by the following formula (1) where four molecules of resveratrol are bonded, the resveratrol being obtained by subjecting a fermentation liquid to a protease treatment, followed by separation and purification by an ion exchange resin and the like, the fermentation liquid being obtained by adding Bacillus natto and Monascus purpureus to sprout-containing grape seeds and soybean powder and fermenting the mixture.EFFECT: The resveratrol derivative shows an amphiphilic property including hydrophobicity inside the molecule and hydrophilicity outside the molecule. The hydroxy group at 4-position of each resveratrol is free, and these hydroxy groups generate hydrogen, reduces cells, passes through cell membranes, activates genes of a keratin generating enzyme to increase production of keratin, suppresses decomposition of keratin, and activates proliferation of epithelial cells.SELECTED DRAWING: None

Functional Complementation of Anti-Adipogenic Phytonutrients for Obesity Prevention and Management

Abstract: Obesity is an established risk factor for metabolic disease. This study explores the functional complementation of anti-adipogenic phytonutrients for obesity prevention and management. Nine phytonutrients were selected based on their ability to affect the expression of one or more selected adipogenic biomarker proteins. The phytonutrients include berberine, luteolin, resveratrol, fisetin, quercetin, fucoidan, epigallocatechin gallate, hesperidin, and curcumin. The selected adipogenic biomarker proteins include PPARɣ, SREBP1c, FASN, PLIN1, FABP4, and β-catenin. Individually, phytonutrients had variable effects on the expression level of selected adipogenic biomarker proteins. Collectively, the functional complementation of nine phytonutrients suppressed de novo fatty acid biosynthesis via the negative regulation of PPARɣ, FASN, PLIN1, and FABP4 expression; activated glycolysis via the positive regulation of SREBP1c expression; and preserved cell–cell adhesion via the inhibition of β-catenin degradation. In primary human subcutaneous preadipocytes, the composition of nine phytonutrients had more potent and longer lasting anti-adipogenic effects compared to individual phytonutrients. In a diet-induced obesity murine model, the composition of nine phytonutrients improved glucose tolerance and reduced weight gain, liver steatosis, visceral adiposity, circulating triglycerides, low-density lipoprotein cholesterol, and inflammatory cytokines and chemokines. The functional complementation of anti-adipogenic phytonutrients provides an effective approach toward engineering novel therapeutics for the prevention and management of obesity and metabolic syndrome.

Effects of native and particulate polyphenols on DNA damage and cell viability after UV-C exposure.

Abstract: Plant polyphenols have poor water solubility, resulting in low bioavailability. In order to overcome this limitation, the drug molecules can be coated with multiple layers of polymeric materials. Microcrystals of quercetin and resveratrol coated with a (PAH/PSS)4 or (CH/DexS)4 shell were prepared using the layer-by-layer assembly method; cultured human HaCaT keratinocytes were treated with UV-C, and after that, cells were incubated with native and particulate polyphenols. DNA damage, cell viability, and integrity were evaluated by comet assay, using PrestoBlueTM reagent and lactate dehydrogenase (LDH) leakage test. The data obtained indicate that both native and particulate polyphenols added immediately after UV-C exposure increased cell viability in a dose-dependent manner; however, the efficiency of particulate quercetin was more pronounced than that of the native compound; also quercetin coated with a (CH/DexS)4 shell more effectively than the native compound reduced the number of DNA lesions in the nuclei of keratinocytes exposed to UV-C radiation; native and particulate resveratrol were ineffective against DNA damage. Quercetin reduces cell death caused by UV-C radiation and increases DNA repair capacity. Coating quercetin with (CH/DexS)4 shell markedly enhanced its impact on DNA repair.