Rice bran oil

About: Rice bran oil is a research topic. Over the lifetime, 2102 publications have been published within this topic receiving 32504 citations.

Protection by pure and genistein fortified extra virgin olive oil, canola oil, and rice bran oil against acetic acid-induced ulcerative colitis in rats

Abstract: Genistein is a major isoflavone with antioxidant and anti-inflammatory activities and hydrophobic features. To increase its bioavailability, researchers supplemented genistein with dietary oils. The present study aims to compare the effectiveness of genistein supplementation with water solution or oils and find the best possible dietary oil for fortification. A total of 135 male Sprague-Dawley rats were randomly assigned to nine groups, including one control group and eight acetic acid-induced colitis groups. The rats in the intervention groups were treated with 5 ml per kg body weight of oral pure and genistein fortified forms of extra virgin olive oil (EVOO), canola oil (CaO), and rice bran oil (RBO); the Genistein group (G) received 100 mg per kg body weight of genistein in aqueous solution orally. The colitis and control groups did not receive any treatment. The levels of colonic MDA (malondialdehyde), MPO (myeloperoxidase) activity, and IL-1β (interleukin-1β) were evaluated and a stereological analysis of colonic tissues was performed. All of the dietary oils (EVOO, CaO, and RBO) were effective at ameliorating the rats' oxidative and inflammatory status (p < 0.05); however, EVOO (with or without genistein) prescription resulted in slightly better results, especially with regard to the inflammatory profile. Additionally, delivering genistein via an oil vehicle was more efficient at reducing MDA formation, MPO activity, and IL-1β concentration than genistein in aqueous solution. The quantitative analysis of colonic tissue was consistent with the biochemical analysis. Our findings suggest that the oral administration of EVOO, canola oil, and rice bran oil can attenuate the elevated IL-1β levels and oxidative damage in induced colitis. Moreover, genistein fortified oils, especially EVOO, showed more beneficial effects in decreasing these markers in comparison with the pure oils or genistein (aqueous solution).

Subcritical dimethyl ether extraction as a simple method to extract nutraceuticals from byproducts from rice bran oil manufacture.

Abstract: The byproducts of rice bran oil processes are a good source of fat-soluble nutraceuticals, including γ-oryzanol, phytosterol, and policosanols. This study aimed to investigate the effects of green technology with low pressure as the subcritical fluid extraction with dimethyl ether (SUBFDME) on the amount of γ-oryzanol, phytosterol, and policosanol extracted from the byproducts and to increase the purity of policosanols. The SUBFDME extraction apparatus was operated under pressures below 1 MPa. Compared to the chemical extraction method, SUBFDME gave the highest content of γ-oryzanol at 924.51 mg/100 g from defatted rice bran, followed by 829.88 mg/100 g from the filter cake, while the highest phytosterol content was 367.54 mg/100 g. Transesterification gave the highest extraction yield of 43.71% with the highest policosanol content (30,787 mg/100 g), and the SUBFDME method increased the policosanol level from transesterified rice bran wax to 84,913.14 mg/100 g. The results indicate that the SUBFDME method is a promising tool to extract γ-oryzanol and phytosterol and a simple and effective technique to increase the purity of policosanol. The study presented a novel technique for the potential use of SUBSFDME as an alternative low-pressure and low-temperature technique to extract γ-oryzanol and phytosterol. The combination of transesterification and the SUBFDME technique is a potential simple two-step method to extract and purify policosanol, which is beneficial for the manufacture of dietary supplements, functional foods and pharmaceutical products.

Rapid reversed-phase chromatographic method for determination of eight vitamin E isomers and γ-oryzanols in rice bran and rice bran oil.

Abstract: Rice bran and rice bran oil contain high amounts of lipid soluble phytochemicals such as tocopherols, tocotrienols and γ-oryzanols with potential health benefits. A reversed-phase high-performance liquid chromatographic method for the quantitation of these phytochemicals was developed. These phytochemicals were extracted using rapid one-step methanol extraction and separated on a Kinetex pentafluorophenyl phase column for quantitation using fluorescence and ultraviolet detectors. Separation was carried out within 20 min at a flow rate of 1.0 mL/min using a gradient elution of mobile phase consisting of methanol and water at column temperature of 30 °C. A good separation of all eight tocol isomers was achieved while the γ-oryzanols were separated near to baseline. Extraction yields of tocopherols, tocotrienols and γ-oryzanols from saponification medium with different pH conditions were studied. Total γ-oryzanols extracted from alkaline medium were significantly lower than those from the neutral and acidic mediums (p < 0.05), while total tocols extracted from these mediums were not significantly different. The method gave a good calibration linear response (R 2 > 0.9994), low detection limit (3–34 ng/mL) and high precision (RSD < 7.5 %) with satisfactory recovery (R% = 81–116 %). The advantages of this method over available reverse-phase methods are the better resolution of β and γ tocol isomers with the γ-oryzanols, usage of non-halogenated mobile phase and shorter analysis time, and it is compatible with the standard laboratorial chromatographic equipment.