Topic
RNA
About: RNA is a research topic. Over the lifetime, 111695 publications have been published within this topic receiving 5475262 citations. The topic is also known as: ribonucleic acid.
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TL;DR: Homology-dependent gene silencing was shown to be used to inhibit Stellate gene expression in the D. melanogaster germline, ensuring male fertility and dsRNA-mediated silencing may provide a basis for negative autogenous control of gene expression.
738 citations
TL;DR: In this paper, a comparative study of optimized antisense oligonucleotides designed to work by an RNA interference mechanism to oligon nucleotide-dependent mechanisms in human cells was performed and the potency, maximal effectiveness, duration of action, and sequence specificity of optimized RNase H-dependent oligonuclotide and small interfering RNA (siRNA) oligoneucleotide duplexes were evaluated and found to be comparable.
738 citations
TL;DR: The construction and application of a shRNA expression library targeting 9,610 human and 5,563 mouse genes is reported, which suggests that the large-scale RNAi library can be used in specific, genetic applications in mammals, and will become a valuable resource for gene analysis and discovery.
Abstract: Gene silencing by RNA interference (RNAi) in mammalian cells using small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) has become a valuable genetic tool. Here, we report the construction and application of a shRNA expression library targeting 9,610 human and 5,563 mouse genes. This library is presently composed of about 28,000 sequence-verified shRNA expression cassettes contained within multi-functional vectors, which permit shRNA cassettes to be packaged in retroviruses, tracked in mixed cell populations by means of DNA 'bar codes', and shuttled to customized vectors by bacterial mating. In order to validate the library, we used a genetic screen designed to report defects in human proteasome function. Our results suggest that our large-scale RNAi library can be used in specific, genetic applications in mammals, and will become a valuable resource for gene analysis and discovery.
736 citations
TL;DR: RNA helicases from the DEAD-box family are found in almost all organisms and have important roles in RNA metabolism, associated with many processes ranging from RNA synthesis to RNA degradation.
Abstract: RNA helicases from the DEAD-box family are found in almost all organisms and have important roles in RNA metabolism. They are associated with many processes ranging from RNA synthesis to RNA degradation. DEAD-box proteins use the energy from ATP hydrolysis to rearrange inter- or intra-molecular RNA structures or dissociate RNA–protein complexes. Such dynamic rearrangements are fundamental for many, if not all, steps in the life of an RNA molecule. Recent biochemical, genetic and structural data shed light on how these proteins power the metabolism of RNA within a cell.
736 citations
TL;DR: This work has identified a class of riboswitches that selectively recognizes guanine and becomes saturated at concentrations as low as 5 nM, and it is now apparent that ribosWitches contribute to the regulation of numerous fundamental metabolic pathways in certain bacteria.
736 citations