Topic
RNA-dependent RNA polymerase
About: RNA-dependent RNA polymerase is a research topic. Over the lifetime, 13904 publications have been published within this topic receiving 767954 citations. The topic is also known as: RdRp & RNA replicase.
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TL;DR: The structural polypeptides of fowl plague virus (influenza A) and those synthesized in fowl Plague virus-infected chick embryo fibroblasts have been analyzed by high resolution polyacrylamide gel electrophoresis and shown to be distinct by tryptic peptide mapping.
209 citations
TL;DR: The heterotrimeric RNA-dependent RNA polymerase of influenza viruses catalyzes RNA replication and transcription activities in infected cell nuclei, and the activity is highly host- and cell type-specific, being dependent on the identity of a few key amino acid positions in the different subunits.
209 citations
TL;DR: A detailed mutagenesis analysis of NS5A domains II and III using the genotype 1b HCV replicon system indicates that the entire domain III region and large portions of domain II of theNS5A protein are not required for the function of NS 5A in HCV RNA replication.
Abstract: The NS5A protein of hepatitis C virus (HCV) plays an important but undefined role in viral RNA replication. NS5A has been proposed to be a three-domain protein, and the crystal structure of the well-conserved amino-terminal domain I has been determined. The remaining two domains of NS5A, designated domains II and III, and their corresponding interdomain regions are poorly understood. We have conducted a detailed mutagenesis analysis of NS5A domains II and III using the genotype 1b HCV replicon system. The majority of the mutants containing 15 small (8- to 15-amino-acid) deletions analyzed were capable of efficient RNA replication. Only five deletion mutations yielded lethal phenotypes, and these were colinear, spanning a 56-amino-acid region within domain II. This region was further analyzed by combining triple and single alanine scanning mutagenesis to identify individual residues required for RNA replication. Based upon this analysis, 23 amino acids were identified that were found to be essential. In addition, two residues were identified that yielded a small colony phenotype while possessing only a moderate defect in RNA replication. These results indicate that the entire domain III region and large portions of domain II of the NS5A protein are not required for the function of NS5A in HCV RNA replication.
209 citations
TL;DR: Fractionation of a transcription-competent HeLa cell extract on a column containing one copy of the heptamer repeat present in the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II resulted in the loss of transcriptional activity.
209 citations
TL;DR: This work presents a new approach to quantifying the importance of the number of components in the response of the immune system to virus infection.
208 citations