Topic
RNA-dependent RNA polymerase
About: RNA-dependent RNA polymerase is a research topic. Over the lifetime, 13904 publications have been published within this topic receiving 767954 citations. The topic is also known as: RdRp & RNA replicase.
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TL;DR: It is shown that Ded1p purified from Escherichia coli has an ATPase activity, which is stimulated by various RNA substrates, and that cells harboring a Ded1 protein with this mutated DEAD motif are nonviable, suggesting that the ATPase and RNA helicase activities of this protein are essential to the cell.
199 citations
TL;DR: It is suggested that sigma 70 remains bound to core RNA polymerase when the -10 promoter contacts are broken, and then moves to the pause-inducing sequence, which carries out base-specific recognition of the nontemplate strand as single stranded DNA.
199 citations
TL;DR: Primary structure analyses of the two leader transcripts revealed a well-conserved pattern of RNA base paring and suggest a model for regulation of transcription termination that is based on ribosome movement along the RNA and a shift between alternative RNA base-pairing configuration.
Abstract: Transcription termination at the attenuators of the trp operons of Escherichia coli and Salmonella typhimurium was studied in vitro using DNA restriction fragments as templates. Readthrough transcription beyond the terminators occurred with 5 and 30% efficiency, respectively, in E. coli and S. typhimurium. This difference is correlated with the stability of proposed secondary structures of the respective trp leader transcripts. Secondary structure analyses of the two leader transcripts revealed a well-conserved pattern of RNA base paring. This and the possibility that trp leader RNA is translated suggest a model for regulation of transcription termination that is based on ribosome movement along the RNA and a shift between alternative RNA base-pairing configuration.
199 citations
TL;DR: The autoantigen La binds the U‐rich 3′ ends of all nascent RNA polymerase III transcripts and appears to be required for their synthesis.
Abstract: The autoantigen La binds the U-rich 3' ends of all nascent RNA polymerase III transcripts Here, we demonstrate that this abundant nuclear phosphoprotein not only binds these RNAs but appears to be required for their synthesis HeLa cell extracts immunochemically depleted of La by either patient or mouse monoclonal antibodies lose greater than 99% of their transcription activity on class III genes The few transcripts synthesized in the absence of La have fewer uridylate residues at their 3' ends than those made in its presence Reconstitution of La-depleted extracts with biochemically purified HeLa La protein stimulates transcription levels and completely restores transcript length A model coupling transcription levels to the action of La at the RNA polymerase III termination signal is presented
199 citations
Patent•
02 Aug 2000
TL;DR: In this article, the 3' region of the RNA is able to base-pair with at least 8 bases at the 5' terminus of the same RNA molecule, provided that the RNA molecule has at least two bases at each of the bases.
Abstract: A transcribed non-naturally occuring RNA molecule comprising a desired RNA molecule, wherein the 3' region of the RNA is able to base-pair with at least 8 bases at the 5' terminus of the same RNA molecule.
199 citations