scispace - formally typeset
Search or ask a question
Topic

rpoB

About: rpoB is a research topic. Over the lifetime, 1947 publications have been published within this topic receiving 61401 citations. The topic is also known as: RNA_pol_bsu_bac & IPR010243.


Papers
More filters
Journal ArticleDOI
TL;DR: Substitution of a limited number of highly conserved aminoacids encoded by the rpoB gene appears to be the molecular mechanism responsible for "single step" high-level resistance to rifampicin in M tuberculosis, a marker of multidrug-resistant tuberculosis.

1,284 citations

Journal ArticleDOI
TL;DR: A comprehensive analysis of Rifr mutations to identify their structural and functional effects on RNA polymerase and discusses the implications of the results with regards to the structure of the rifampicin binding site.

646 citations

Journal ArticleDOI
TL;DR: Molecular strategies show considerable promise for rapid detection of mutations associated with antimicrobial resistance and are now amenable to utilization in an appropriately equipped clinical microbiology laboratory.
Abstract: The primary theme emerging from molecular genetic work conducted with Mycobacterium tuberculosis and several other mycobacterial species is that resistance is commonly associated with simple nucleotide alterations in target chromosomal genes rather than with acquisition of new genetic elements encoding antibiotic-altering enzymes. Mutations in an 81-bp region of the gene (rpoB) encoding the beta subunit of RNA polymerase account for rifampin resistance in 96% of M. tuberculosis and many Mycobacterium leprae isolates. Streptomycin resistance in about one-half of M. tuberculosis isolates is associated with missense mutations in the rpsL gene coding for ribosomal protein S12 or nucleotide substitutions in the 16S rRNA gene (rrs). Mutations in the katG gene resulting in catalase-peroxidase amino acid alterations nad nucleotide substitutions in the presumed regulatory region of the inhA locus are repeatedly associated with isoniazid-resistant M. tuberculosis isolates. A majority of fluoroquinolone-resistant M. tuberculosis isolates have amino acid substitutions in a region of the DNA gyrase A subunit homologous to a conserved fluoroquinolone resistance-determining region. Multidrug-resistant isolates of M. tuberculosis arise as a consequence of sequential accumulation of mutations conferring resistance to single therapeutic agents. Molecular strategies show considerable promise for rapid detection of mutations associated with antimicrobial resistance. These approaches are now amenable to utilization in an appropriately equipped clinical microbiology laboratory.

620 citations

Journal ArticleDOI
TL;DR: The single-copy gene rpoB provided comparable phylogenetic resolution to that of the 16S rRNA gene at all taxonomic levels, except between closely related organisms (species and subspecies levels), for which it provided better resolution.
Abstract: Several characteristics of the 16S rRNA gene, such as its essential function, ubiquity, and evolutionary properties, have allowed it to become the most commonly used molecular marker in microbial ecology However, one fact that has been overlooked is that multiple copies of this gene are often present in a given bacterium These intragenomic copies can differ in sequence, leading to identification of multiple ribotypes for a single organism To evaluate the impact of such intragenomic heterogeneity on the performance of the 16S rRNA gene as a molecular marker, we compared its phylogenetic and evolutionary characteristics to those of the single-copy gene rpoB Full-length gene sequences and gene fragments commonly used for denaturing gradient gel electrophoresis were compared at various taxonomic levels Heterogeneity found between intragenomic 16S rRNA gene copies was concentrated in specific regions of rRNA secondary structure Such “heterogeneity hot spots” occurred within all gene fragments commonly used in molecular microbial ecology This intragenomic heterogeneity influenced 16S rRNA gene tree topology, phylogenetic resolution, and operational taxonomic unit estimates at the species level or below rpoB provided comparable phylogenetic resolution to that of the 16S rRNA gene at all taxonomic levels, except between closely related organisms (species and subspecies levels), for which it provided better resolution This is particularly relevant in the context of a growing number of studies focusing on subspecies diversity, in which single-copy protein-encoding genes such as rpoB could complement the information provided by the 16S rRNA gene

604 citations

Journal ArticleDOI
TL;DR: RpoB is a powerful identification tool, which may be useful for universal bacterial identification, and the trees obtained with rpoB were more compatible with the currently accepted classification of Enterobacteriaceae than those obtained with 16S rRNA.
Abstract: Summary Comparison of the sequences of conserved genes, most commonly those encoding 16S rRNA, is used for bacterial genotypic identification. Among some taxa, such as the Enterobacteriaceae, variation within this gene does not allow confident species identification. We investigated the usefulness of RNA polymerase beta-subunit encoding gene (rpoB ) sequences as an alternative tool for universal bacterial genotypic identification. We generated a database of partial rpoB for 14 Enterobacteriaceae species and then assessed the intra- and interspecies divergence between the rpoB and the 16S rRNA genes by pairwise comparisons. We found that levels of divergence between the rpoB sequences of different strains were markedly higher than those between their 16S rRNA genes. This higher discriminatory power was further confirmed by assigning 20 blindly selected clinical isolates to the correct enteric species on the basis of rpoB sequence comparison. Comparison of rpoB sequences from Enterobacteriaceae was also used as the basis for their phylogenetic analysis and demonstrated the genus Klebsiella to be polyphyletic. The trees obtained with rpoB were more compatible with the currently accepted classification of Enterobacteriaceae than those obtained with 16S rRNA. These data indicate that rpoB is a powerful identification tool, which may be useful for universal bacterial identification.

571 citations


Network Information
Related Topics (5)
Bacteria
23.6K papers, 715.9K citations
86% related
Polymerase chain reaction
18.4K papers, 768.7K citations
86% related
Mycobacterium tuberculosis
25.1K papers, 846K citations
86% related
Virulence
35.9K papers, 1.3M citations
85% related
Antibiotic resistance
29.1K papers, 884.5K citations
84% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023105
2022235
202185
202093
201981
201895