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Showing papers on "Sativum published in 1973"





Journal ArticleDOI
01 Nov 1973-Botany
TL;DR: Protoplasts isolated from mesophyll of Pisum sativum L. cv.
Abstract: Protoplasts isolated from mesophyll of Pisum sativum L. cv. Century, and cultured in 0.2-ml droplets of B5 medium with 1.0 mg/liter (2,4-dichlorophenoxy)acetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and 2.0 mg/liter kinetin regenerated cell walls within 2–3 days. The resulting cells began to divide and form calli after 19 days of culture. Protoplast survival depended on keeping the leaf material in the dark for at least 30 h before use.

60 citations


Journal ArticleDOI
TL;DR: A methanolic extract of tubers of Cyperus esculentus Ten.
Abstract: A methanolic extract of tubers of Cyperus esculentus Ten. var. aureus Richt. contains compounds which inhibit growth of oat coleoptile sections and germination of Beta vulgaris L., Lotus corniculatus L., Lolium perenne L., Pisum sativum L., Trifolium repens L., Lactuca sativa L. and Lycopersicum esculentum Ludwig. p-Hydroxybenzoic acid, vanillic acid, syringic acid, ferulic acid and p-coumaric acid were identified. Four other active compounds which could not be identified were also isolated.

37 citations


Journal ArticleDOI
01 Mar 1973-Planta
TL;DR: The gibberellin A1 (GA1)-like and GA5-like fractions from immature seeds of Pisum sativum cv.
Abstract: The gibberellin A1 (GA1)-like and GA5-like fractions from immature seeds of Pisum sativum cv. Progress No. 9 were identified by combined gas chromatography-mass spectrometry as GA29 and GA20 respectively.

31 citations


Journal ArticleDOI
TL;DR: The identification of GA3 was made by gas-liquid radiochromatography using three stationary phases as mentioned in this paper, and two acidic compounds, GA3 and a chromatographically similar unknown.

29 citations


Journal ArticleDOI
TL;DR: Germinating pea seedlings ( Pisum sativum var. Alaska) contain high concentrations of d -alanine, which occurs in the decotyledonized parts as the conjugates as mentioned in this paper.

27 citations


Journal ArticleDOI
TL;DR: Stoichiometric yields of indolepyruvate and glutamate were obtained with d,l-tryptophan and alpha-ketoglutarate as co-substrates, and the specific activity was three times higher with d-t Kryptophan than with l-tiptophan.
Abstract: A transaminase (aminotransferase, EC 2.6.1) fraction was partially purified from shoot tips of pea (Pisum sativum L. cv. Alaska) seedlings. With alpha-ketoglutarate as co-substrate, the enzyme transaminated the following aromatic amino acids: d,l-tryptophan, d,l-tyrosine, and d,l-phenylalanine, as well as the following aliphatic amino acids: d,l-alanine, d,l-methionine, and d,l-leucine. Of other alpha-keto acids tested, pyruvate and oxalacetate were more active than alpha-ketoglutarate with d,l-tryptophan. Stoichiometric yields of indolepyruvate and glutamate were obtained with d,l-tryptophan and alpha-ketoglutarate as co-substrates. The specific activity was three times higher with d-tryptophan than with l-tryptophan.

25 citations


Journal ArticleDOI
TL;DR: Amino acid analysis of NPN fractions of meals obtained by method 1 showed the oilseed meals and peas to contain, in free state, all the protein amino acids except cystin...
Abstract: Nonprotein nitrogen (NPN) was extracted from seven species of oilseeds and three cultivars of peas (Pisum sativum L.) by three methods. Method 1 was extraction of meal nitrogen with dilute sodium h...

21 citations




Journal ArticleDOI
01 Mar 1973-Planta
TL;DR: The main gibberellin in immature seed of Pisum sativum L., cv.
Abstract: The main gibberellin in immature seed of Pisum sativum L., cv. Alaska, is identified as GA20 by GC-MS. GA9 may also be present.


Journal ArticleDOI
TL;DR: Prisposition to hollow heart in pea seeds is caused by high ambient temperatures during their maturation on the plant, and by drying them when immature, and it is suggested that predisposed cells die during rehydration.
Abstract: SUMMARY Predisposition to hollow heart in pea seeds (Pisum sativum L.) is caused by high ambient temperatures during their maturation on the plant, and by drying them when immature. Symptoms develop during germination, and the proportion of seeds affected in any sample depends on the rate of water imbibition. It is suggested that predisposed cells die during rehydration.

Journal ArticleDOI
TL;DR: A fully normal meiosis was found in Allium sativum L. by means of PMC analysis and evidence for the pollen sterility was given by crossings on sterile types.
Abstract: A fully normal meiosis was found inAllium sativum L. by means of PMC analysis. Quadrivalents were formed only in several cultivars. The cultivation of inflorescence stem (after the exstirpation of bulblets) in a tetracycline solution of 900 i.u. induced a formation of fertile flowers. The first pollen mitosis was studied and the pollen parameters compared with those ofAllium cepa L. Evidence for the pollen sterility was given by crossings on sterile types. A microbiological factor was not isolated.


Journal ArticleDOI
01 Jun 1973-Planta
TL;DR: The results support the supposition that the GDH-I andGDH-II isoenzyme patterns correspond to different molecular forms of one enzyme, the GD H-II representing a form with predominantly anabolic function and the GDh-I a form which has merely metabolic or catabolic function.
Abstract: The isoenzymes of NAD-specific glutamic dehydrogenase (GDH) of Pisum sativum, separated by polyacrylamide gel electrophoresis, constitute two patterns, each of which covers seven individual isoenzymes. One pattern (GDH-I) is found in the cotyledons and young shoots. The second one (GDH-II) occurs together with at least some GDH-I isoenzymes in pea roots. In the shoots of older pea plants GDH-II isoenzymes become visible in addition to the GDH-I pattern.

Journal ArticleDOI
TL;DR: An investigation of four mutant genotypes of P. sativum has shown an inverse relationship between peroxidase activity and lignification of the pod membrane.
Abstract: An investigation of four mutant genotypes of P. sativum has shown an inverse relationship between peroxidase activity and lignification of the pod membrane. From developmental studies, it appears that the main role of peroxidase is in cellular growth and differentiation.

Journal ArticleDOI
TL;DR: 5-oxoproline, found in large amounts in the young samples, was shown to be an artifact produced from glutamine during killing and analysis and a two- to threefold range in content of the individual acids was found.
Abstract: The content of the major organic acids of 6 cultivars of green pea at a range of maturities was determined. On a fresh weight basis the changes in the acids were small except for citrate which rose and then fell again. Ten other varieties of pea were examined at one maturity and a two- to threefold range in content of the individual acids was found. 5-oxoproline, found in large amounts in the young samples, was shown to be an artifact produced from glutamine during killing and analysis.

Journal ArticleDOI
TL;DR: The results suggest that, with the possible exception of corn leaf samples from one location, Zn levels in plant tissue were adequate, however, the results indicate that liming strongly acid sandy soils reduces Zn availability and may induce a deficiency in Zn sensitive crops.
Abstract: Ranges for total, 0.1N HCl and EDTA‐(NH4)2CO3 extractable Zn in 69 samples of surface soil (0–15 cm), representing nine soil series, were 14–108, 0.9–10.5 and 0.5–8.0 ppm respectively. Total Zn in barley (Hordeum vulgare), carrot (Daucus carata sativa), corn (Zea mays L), grape (Vitis spp.), onion (Allium cepa), pea (Pisum sativum, strawberry (Fragaria spp.), and wheat (Triticum spp.) leaves and in barley and wheat grain ranged from 13.5 to 80.6 ppm. The results suggest that, with the possible exception of corn leaf samples from one location, Zn levels in plant tissue were adequate. However, the results also indicate that liming strongly acid sandy soils reduces Zn availability and may induce a deficiency in Zn sensitive crops.

Journal ArticleDOI
TL;DR: Plants from seeds with the disorder were smaller and yielded less than those from normal seeds, and evidence suggested the presence of a germination and growth inhibitor.
Abstract: SUMMARY Hollow heart of pea seed (Pisum sativum L.) delayed germination and reduced seedling growth. Plants from seeds with the disorder were smaller and yielded less than those from normal seeds. Affected cells were dead, but immobilization of starch reserves within them could not wholly account for the reduced growth and evidence suggested the presence of a germination and growth inhibitor.


Journal ArticleDOI
TL;DR: Rhynchosporium orthosporum was isolated from Lolium multifiorum from breeders’ plots at Aberystwyth twice as often as R. secalis, suggesting that there are reasonable prospects for resistance breeding.
Abstract: SUMMARY Rhynchosporium orthosporum was isolated from Lolium multifiorum from breeders’ plots at Aberystwyth twice as often as R. secalis. R. orthosporum from Dactylis glomerata, L. multiflorum or L. perenne would infect only the host genus from which it was isolated. R. secalis from L. multiflorum would produce symptoms not only on L. perenne, but on Hordeum sativum as well. Significantly, this was of race U.K.1, the most common race on H. sativum. Of 20 genotypes of L. multifiorum cv. RvP, 17 ranked similarly for level of resistance to both pathogen species. However, the remaining three genotypes were resistant to one, but markedly susceptible to the other. Progeny of selected resistant genotypes were far more resistant than the original cultivar. This suggests that there are reasonable prospects for resistance breeding.

Journal ArticleDOI
TL;DR: In roots of pea seedlings grown in medium containing aldrin, the disappearance of a Aldrin was greater than the production of dieldrin, including that which could be accounted for by transport to the tops.
Abstract: A study has been made of the rate of aldrin metabolism by a number of plant species. In roots of pea seedlings grown in medium containing aldrin, the disappearance of aldrin was greater than the production of dieldrin, including that which could be accounted for by transport to the tops.



Journal Article
TL;DR: The mode of inheritance of resistance to the Pusa isolate of H. sativum was studied in a diallel set of crosses involving four varieties viz., EB.
Abstract: The mode of inheritance of resistance to the Pusa isolate of H. sativum was studied in a diallel set of crosses involving four varieties viz., EB. 132, E 1912, EB 2110 and EB 1467. The resistance to H. sativum was observed to be recesssve in expression. Resistance of each of the parent is controlled by a single recessive gene pair. The resistant genes found in the three resistant parents were different from each other.

Journal ArticleDOI
30 Jun 1973
TL;DR: Sordaria fimicola was the only fungus which restricted the growth of Helminthosporium sativum, H. triseptatum, Fusarium nivale and F. avenaceum.
Abstract: The biotic relations were evaluated between saprophytic fungi genera Fusarium and Helmithosporium . Most of the saprophytic fungi restricted the development of Helmihthosporium sativum and H. triseptatum more than that of Fusarium nivale and F. avenaceum. Sordaria fimicola was the only fungus which restricted the growth of Helminthosporium sativum, H. triseptatum, Fusarium nivale and F. avenaceum .