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Showing papers on "Sativum published in 1974"


Journal ArticleDOI
TL;DR: Callus formation and shoot production occurred within 4 to 6 weeks in defined media containing 0.2 to 5.0 μM benzyladenine and 1 μM naphthaleneacetic acid while most callus produced one or more shoots at high frequency, root formation did not occur regularly.
Abstract: Shoot formation was observed in callus from apical cells of pea (Pisum sativum L. cv. Century). Shoot apices from 4-day-old plants were macerated and the resulting cell masses grown on agar media. The callus formation and shoot production occurred within 4 to 6 weeks in defined media containing 0.2 to 5.0 μM benzyladenine and 1 μM naphthaleneacetic acid. While most callus produced one or more shoots at high frequency, root formation did not occur regularly. Plants obtained by these procedures were grown to maturity producing flowers and pods.

114 citations


Journal ArticleDOI
01 Jun 1974-Planta
TL;DR: In addition to the previously identified GA20 and GA29 in immature seeds of Pisum sativum L. cv, Progress No. 9, GA9, GA17, GA38, GA44, abscisic acid and dihydrophaseic acid have been identified.
Abstract: In addition to the previously identified GA20 and GA29 in immature seeds of Pisum sativum L. cv. Progress No. 9, GA9, GA17, GA38, GA44, abscisic acid and dihydrophaseic acid have been identified. The levels of GA9, GA17, GA20 and GA29 have been determined throughout seed maturation by GC-MS. GA20 and GA29 are the major gibberellins in terms of quantity, the other gibberellins remain at very low levels throughout development of the seed.

99 citations


Journal ArticleDOI
TL;DR: Root formation, on the shoots produced by culturing meristems, was induced by reculturing the shoots, 2 cm long, on half strength B5 medium supplemented with NAA at a concentration of 10 −6 M.

59 citations


Journal ArticleDOI
TL;DR: The properties of the amine oxidases of barley leaves and pea seedling cotyledons have been compared using a colorimetric assay in which the hydrogen p was calculated as discussed by the authors.

39 citations


Journal ArticleDOI
TL;DR: The cessation of cell elongation in intact P. sativum epicotyls is accompanied by an increase in both soluble and cell wall peroxidases, which results in a decrease in the number ofocytes in intact cells.

36 citations


Journal ArticleDOI
TL;DR: The karyologic analysis of three strains of the callus cultures of Allium sativum L. var.
Abstract: SUMMARYThe karyologic analysis of three strains of the callus cultures of Allium sativum L. var. sativum derived from leaf tissues of three cultivars is presented. The explants were removed from virus-free plants and cultivated on the synthetic medium. A frequency of individual chromosomal types as related to the length of cultivation in vitro was studied in individual strains of the cultures.

32 citations


Journal ArticleDOI
F. Dorn1, D. Arigoni1
TL;DR: Two new secolongifolane derivative, shown to possess structure5, have been isolated from Helminthosporium sativum and H. victoriae.
Abstract: (−)-Longifolene,4, and a new secolongifolane derivative, shown to possess structure5, have been isolated fromHelminthosporium sativum andH. victoriae.

27 citations


Journal ArticleDOI
TL;DR: Tritium labelled gibberellin A 20 ([ 3 H]-GA 20 ) applied to etiolated shoots and germinating seeds of dwarf pea ( Pisum sativum L. cv. Meteor) was converted to gibberenin A 29. Identifications were made by GLRC and GCMS.

23 citations


Journal ArticleDOI
TL;DR: Uninoculated roots of pea and French bean have been shown to exude a number of antifungal compounds when grown in a non-sterile aqueous aerated medium and their possible importance in relation to disease resistance is discussed.
Abstract: Uninoculated roots of pea (Pisum sativum) and French bean (Phaseolus vulgaris) have been shown to exude a number of antifungal compounds when grown in a non-sterile aqueous aerated medium. These have been identified and their possible importance in relation to disease resistance is discussed.

21 citations


Journal Article
TL;DR: Thermal inactivation of lipoxygenase from peas (Pisum Sativum L.) : 4.
Abstract: Thermal inactivation of lipoxygenase from peas (Pisum Sativum L.). : 4. Inactivation of whole peas

14 citations



Journal Article
TL;DR: In this article, the inactivation of lipoxygenase from peas (Pisum sativum) was investigated using single heat treatment experiments and the results showed that the energy obtained from such experiments was negligible.
Abstract: Thermal inactivation of lipoxygenase from peas (Pisum Sativum L.). : 3. Activation energy obtained from single heat treatment experiments


Journal ArticleDOI
TL;DR: In this article, the effect of removing of supply or sink organs on the distribution of assimilates in two varieties of Garden Pea, Pisum Sativum L. and Lactobacius L.
Abstract: (1974). The Effect of Removing of Supply or Sink Organs on the Distribution of Assimilates in two Varieties of Garden Pea, Pisum Sativum L. Acta Agriculturae Scandinavica: Vol. 24, No. 1, pp. 7-12.

Journal ArticleDOI
TL;DR: It is probable that disease and senescence symptoms are confused in the field, since the most common disease symptoms, acropetal progression of chlorosis and necrosis of the leaves followed by premature defoliation are indistinguishable from naturalsenescence.
Abstract: SUMMARY A wilt disease of garden pea (Pisum sativum) caused by Verticillium dahliae is described and the range of pathogenicity of the isolate investigated. It is pathogenic to potato, sweet pea, antirrhinum and broad bean and isolates of V. dahliae from potato, lucerne and sweet pea and V. albo-atrum from lucerne are pathogenic to pea. Since the most common disease symptoms, acropetal progression of chlorosis and necrosis of the leaves followed by premature defoliation are indistinguishable from natural senescence, it is probable that disease and senescence symptoms are confused in the field. The premature defoliation results in marked reduction in green leaf area, leaf dry weight and pod yield.


Journal ArticleDOI
01 Mar 1974-Planta
TL;DR: Radioactivity from [Me-3H]thymidine, supplied to the roots of the seedlings, was specifically incorporated into pea DNA, and could not be accounted for by net DNA synthesis and cell division, and is taken as strong evidence for DNA turnover.
Abstract: Pea seedlings (Pisum sativum L.) were grown aseptically in a sterile flexible film animal isolator. Radioactivity from [Me-3H]thymidine, supplied to the roots of the seedlings, was specifically incorporated into pea DNA. There was no incorporation into RNA or into bacterial DNA; neither was there any non-specific binding of label to carbohydrate. Much of the radioactivity incorporated into the DNA was lost during a cold chase. The loss could not be accounted for by net DNA synthesis and cell division, and is therefore taken as strong evidence for DNA turnover. The significance of these results is discussed.


Journal ArticleDOI
TL;DR: Clover yellow mosaic virus multiplication was studied in roots of Pisum sativum (garden pea).
Abstract: Clover yellow mosaic virus (CYMV) multiplication was studied in roots of Pisum sativum (garden pea). Mechanical inoculation of pea roots with CYMV resulted in systemic infection of shoots. CYMV translocation from roots was directly related to temperature. CYMV was purified from pea roots with minimal alterations of existing schedules; yields were comparable to those from leaves (45 mg/kg, roots; 50 mg/kg, leaves). Electron-microscopic observations of thin-sectioned CYMV-infected root-tip tissue revealed large numbers of viral inclusions in meristematic root initials. Nitrogen and carbohydrate levels were significantly altered in roots and shoots of infected seedlings.

Journal ArticleDOI
01 Jan 1974-Planta
TL;DR: Application of the cytokinin, N6-benzyladenine, to light-grown shoots of dwarf “Meteor” pea seedlings (Pisum sativum L.) markedly increased the rate of turnover of GA20, a native pea gibberellin.
Abstract: Application of the cytokinin, N(6)-benzyladenine, to light-grown shoots of dwarf "Meteor" pea seedlings (Pisum sativum L.) markedly increased the rate of turnover of GA20, a native pea gibberellin. It is suggested that endogenous cytokinins may affect gibberellin metabolism in plants by controlling rates of gibberellin turnover.

Journal ArticleDOI
TL;DR: It was found that cellulase (C 1 ) acticity in P. infectoria and N. spherica was adaptive in nature whereas Cx activity in the two pathogens was inductive.





01 Jan 1974
TL;DR: In this paper, slices of young pea leaves (Pisum sativum) were treated with muM solutions of alpha-chlorallyl diethyldithiocarbamate, dichloroallyl diisopropylthiocaramate, or S-ethyldipropylTHioccharamate, which resulted in inhibition of incorporation of [1-(14)C]acetate into C(31) alkane and C( 31) secondary alcohol.
Abstract: Treatment of slices of young pea leaves (Pisum sativum) with muM solutions of alpha-chlorallyl diethyldithiocarbamate, dichloroallyl diisopropylthiocarbamate, or S-ethyldipropylthiocarbamate resulted in inhibition of incorporation of [1-(14)C]acetate into C(31) alkane and C(31) secondary alcohol, very little effect on the synthesis of C(26) and C(28) fatty alcohols, and an accumulation of (14)C in shorter chain cuticular lipids, particularly C(22) acid. Higher concentrations of the thiocarbamates caused inhibition of synthesis of C(26) and C(28) fatty alcohols and an accumulation of label in C(22) acid. Further increase in thiocarbamate concentration resulted in inhibition of C(22) acid synthesis also. The three thiocarbamates at muM concentration also inhibited incorporation of [1-(14)C]stearic acid specifically into C(31) alkane and C(31) secondary alcohol. These results suggest that thiocarbamates reduce cuticular lipid formation by a concentration-dependent inhibition of the various chain-elongating enzyme systems.


Journal ArticleDOI
TL;DR: Pigment contents were determined in the seeds of the pea cultivars Little Marvel and Lincoln in both the first and second generations (Rx and R2) that had been exposed to gamma-ray doses of 1, 2, 4...
Abstract: Pigment contents were determined in the seeds of the pea cultivars Little Marvel and Lincoln in both the first and second generations (Rx and R2) that had been exposed to gamma-ray doses of 1, 2, 4...