scispace - formally typeset
Search or ask a question
Topic

Semen

About: Semen is a research topic. Over the lifetime, 14571 publications have been published within this topic receiving 407739 citations. The topic is also known as: come & ejaculate.


Papers
More filters
Journal ArticleDOI
TL;DR: The processes leading to hyperactivation and to the membrane changes associated with capacitation are not tightly interlinked and this finding is considered to be due to hyperactivated motility being associated with flagellar movement, while the CTC assay assesses changes in the Ca2+ levels of the sperm head plasma membrane.
Abstract: While hyperactivated motility is known to be a concomitant of capacitation, and a prerequisite for fertilization, the specific interdependence of capacitation and hyperactivation in human spermatozoa has not been investigated. This study was designed to determine the effect of seminal plasma contamination on the expression of hyperactivated motility and the relationship between hyperactivation and capacitation, since seminal plasma contains decapacitation factor(s). Seminal plasma was obtained by centrifugation of aliquots of liquefied semen layered over 1.5 ml 40.5% Percoll and mixed with human tubal fluid (HTF) medium containing 30 mg/ml human serum albumin (HSA) (HTF) to a final concentration of 5% (v/v) seminal plasma (SP). Motile spermatozoa were isolated from the remainder of the semen by swim-up into either HTF or SP medium. Samples were taken from each treatment immediately post-harvest (0 h) and after 60 min at 37 degrees C (1 h) for hyperactivation and capacitation assessment. The treatments were then divided into two portions, centrifuged and resuspended in either HTF or SP, giving HTF control and SP control treatments and two crossover treatments, 1 h HTF then 1 h SP (H/SP) and 1 h SP then 1 h HTF (SP/H). All tubes were incubated for a further 60 min at 37 degrees C before aliquots were taken for hyperactivation and capacitation assessments. Hyperactivation was estimated using an IVOS v10.6t (Hamilton Thorne Research, Beverly, MA, USA) 60 Hz CASA instrument, and capacitation was estimated using the chlortetracycline (CTC) method. The presence of seminal plasma in the capacitation medium for 60-120 min post-swim-up inhibited the development of hyperactivated motility. This inhibition was reversible, and was not prevented by preincubation for 1 h in HTF medium. There was no difference in the CTC binding patterns between treatments at 2 h, indicating that the capacitation-associated membrane changes were not affected by the presence of a low concentration of seminal plasma. There was no correlation between percentage capacitated and percentage hyperactivated spermatozoa for any treatment. Since the proportions of hyperactivated spermatozoa and capacitated spermatozoa were not related, we conclude that the processes leading to hyperactivation and to the membrane changes associated with capacitation are not tightly interlinked and consider this finding to be due to hyperactivated motility being associated with flagellar movement, while the CTC assay assesses changes in the Ca2+ levels of the sperm head plasma membrane.

118 citations

Journal ArticleDOI
TL;DR: Cryopreservation of semen should be considered as an option to young male patients whose cancer therapy will include potentially gonadotoxic treatment, after improved survival rates increasingly prompt the question of quality of life after therapy, including fertility.
Abstract: In adult oncological patients semen cryopreservation offers the possibility of preserving fertility prior to aggressive therapy that may lead to infertility. The cryopreserved semen can later be used to induce pregnancies in the partner by techniques of assisted fertilization. In adolescent boys the question of fertility is often beyond consideration when the young patient's life is threatened acutely. However, improved survival rates increasingly prompt the question of quality of life after therapy, including fertility. Semen quality is known to be impaired in patients with malignancies and may be further impaired by the process of cryopreservation. Since normal values for semen in adolescents are not known and spermatogenesis may be impaired by the malignant disease, it was unclear whether semen samples from adolescents with malignancies warrant cryopreservation at all. In order to demonstrate the feasibility of semen cryopreservation in adolescent males, we compared the results from 12 pubertal boys aged 14-17 years with those from 17 young adults aged 18-20 years who had similar malignancies and, additionally, to 210 adults with malignancies (> 20 years). Luteinizing hormone serum values were significantly lower in adolescents than in adult patients. Follicle stimulating hormone showed a significant increase with age. Testosterone serum levels and testicular volumes showed similar distribution patterns in adolescent and adult men. Sperm concentrations, sperm motility, and normal sperm morphology in the adolescent patients did not show significant differences compared with adults. Thus cryopreservation of semen should be considered as an option to young male patients whose cancer therapy will include potentially gonadotoxic treatment.

118 citations

Journal ArticleDOI
TL;DR: In this paper, a series of experiments that evaluated spermatozoal motion characteristics, as determined by computerized semen analysis, of extended, cooled stallion semen was conducted. And the results showed that those aliquots utilizing high dilution ratios of extender (>1:2.1, volume of semen: volume of extenders) maintained higher spermato-zoal motility and mean velocity than those using a lower dilution ratio.

118 citations

Journal ArticleDOI
TL;DR: There was significant seasonal variation in both semen quantity and quality among bucks within a breed and individual evaluation of semen necessary to select the most fertile males for breeding.

118 citations


Network Information
Related Topics (5)
Sperm
43.4K papers, 1.3M citations
92% related
Luteinizing hormone
23.9K papers, 756K citations
83% related
Fertility
29.9K papers, 681.1K citations
82% related
Testosterone
23.2K papers, 808K citations
81% related
Polycystic ovary
20.4K papers, 635K citations
78% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20241
2023973
20222,093
2021538
2020530
2019498