Topic
Semen
About: Semen is a research topic. Over the lifetime, 14571 publications have been published within this topic receiving 407739 citations. The topic is also known as: come & ejaculate.
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TL;DR: Osmolality higher than 200mOsmkg(-1) significantly decreased the percentage of motile sperm, while osmolability of 300mO smkg(- 1) or above totally suppressed sperm motility.
99 citations
TL;DR: It is emphasized that there are a number of specific semen analysis variables, each expressing a different aspect of male fertility potential which, when combined in correct proportion, do provide the optimal evaluation of the male fertility status.
Abstract: The aim of this study was to evaluate diagnostic profiles for the assessment of semen analysis data with respect to male fertility potential. Semen samples taken from 208 patients of known fertility and suspected infertility were studied by conventional semen analysis methods. The data throw doubt upon the validity of an approach based on the number of deviations from the normal standard values defined by the World Health Organization. The alternative approach of a specific semen characteristic (particularly morphology) as the major predictor of fertility produced no beneficial results. However, the semen analysis index based on semen volume, sperm count, percentage motility and normal forms resulted in a high accuracy of classification but for only 44% of the cases, with 3% false negatives and 10% false positives using cut-off indices of > or = 0.6 and < or = -1.0 for defining 'fertile' and 'infertile' zones, respectively. In conclusion, it is emphasized that there are a number of specific semen analysis variables, each expressing a different aspect of male fertility potential which, when combined in correct proportion, do provide the optimal evaluation of the male fertility status. However, in order to increase the prognostic potential of the semen sample, new and meaningful parameters must be discovered.
99 citations
TL;DR: In this paper, the authors evaluated the fertility and pregnancy rates after intracytoplasmic sperm injection (ICSI) in infertile couples with severe male infertility and found that fertility was achieved even in older women but were more readily established in younger women producing larger numbers of metaphase II oocytes.
99 citations
TL;DR: A study was conducted to determine an optimum technique for semen cryopreservation and the biological competence of frozen-thawed ferret spermatozoa and sperm motility and normal acrosomal ratings were highest using the PDV diluent, the pelleting method and thawing at 37 degrees C.
Abstract: A study was conducted to determine an optimum technique for semen cryopreservation and the biological competence of frozen-thawed ferret spermatozoa. Fifty-two fresh electroejaculates from 4 males were evaluated for sperm percentage motility, forward progressive motility, motility index (SMI) and acrosomal integrity. To determine the optimum temperature for maintaining sperm motility in vitro and the influence of glycerol on sperm motility, seminal aliquants were diluted in TEST diluent (containing either 0 or 4% glycerol) and maintained at 25 degrees or 37 degrees C. For cryopreservation, semen was diluted in each of 3 cryodiluents (TEST, PDV, BF5F), cooled for 30 min at 5 degrees C and pelleted on solid CO2 or frozen in 0.25 ml straws (20 degrees C/min to -100 degrees C). Following thawing, SMI and acrosomal integrity were determined. Ten females with maximum vulval swelling were given 90 i.u. human chorionic gonadotrophin and laparoscopically inseminated in utero with spermatozoa previously frozen using the optimum diluent and freeze-thaw method. The maintenance temperature of 25 degrees C was superior (P less than 0.05) to 37 degrees C for sustaining sperm motility, and glycerol did not influence (P greater than 0.05) motility for up to 11 h of culture. After thawing, motile spermatozoa were recovered in all treatment groups, but sperm motility and normal acrosomal ratings were highest using the PDV diluent, the pelleting method and thawing at 37 degrees C (P less than 0.05). Seven of the 10 ferrets (70%) inseminated with spermatozoa frozen by this approach became pregnant and produced 31 kits (mean litter size 4.4; range 1-9 kits).(ABSTRACT TRUNCATED AT 250 WORDS)
99 citations
TL;DR: This study investigated the association between occupational pesticide exposure and semen quality among Chinese workers and found that male workers exposed to organophosphate pesticides had poorer semen quality following a work shift.
Abstract: This study investigated the association between occupational pesticide exposure and semen quality among Chinese workers Male workers, 32 who were exposed to organophosphate pesticides and 43 who were not exposed were recruited from two nearby factories and interviewed Following a work shift, semen and urine samples were collected for pesticide metabolite analysis Semen samples were analyzed for sperm concentration, percentage of motility, and percentage of normal structure Within the exposed group, the mean end-of-shift urinary p-nitrophenol levels were 022 and 015 mg/L for the high- and low-exposure subgroups, respectively Linear regression analysis of individual semen parameters revealed a significant reduction of sperm concentration (359 x 10(6) vs 628 x 10(6), p < 001) and percentage of motility (47% vs 57%, p = 003) but not percentage of sperm with normal structure (57% vs 61%, p = 013) Multivariate modeling showed a significant overall shift in the mean semen parameter Occupational exposure to ethylparathion and methamidophos seems to have a moderately adverse effect on semen quality
99 citations