Topic
Semen
About: Semen is a research topic. Over the lifetime, 14571 publications have been published within this topic receiving 407739 citations. The topic is also known as: come & ejaculate.
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TL;DR: Testing the applicability of a novel fluorescent probe, Mitotracker Deep Red 633, for measuring the mitochondrial activity of spermatozoa both after freezing/thawing and after swim-up selection found it to be a reliable marker for frozen-thawed bovine semen viability both PT and after SU.
94 citations
TL;DR: In this article, the sperm chromatin structure assay (SCSA), lipid peroxidation, superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in seminal plasma and spermatozoa were measured by spectrophotometric assays.
94 citations
TL;DR: It is concluded that the apparent selection of morphologically normal spermatozoa is not a direct function of the female tract, but that the spermutozoa can effect their own selection because of their differential motility.
Abstract: Light microscopic assessment of human spermatozoa in post-coital samples of cervical mucus revealed a significant improvement in the general sperm morphology between the semen and the cervix. Further analysis showed that the excluded spermatozoa were more likely to be those with midpiece or tail defects that impaired motility. Significant changes were also found when the morphology of spermatozoa recovered from the uterus and Fallopian tubes following AIH was compared with the semen used for insemination: in the semen, uterus and oviducts there were respectively 53, 77 and 71% spermatozoa with completely normal morphology, and 12, 3 and 0.6% spermatozoa with defects of the midpiece and/or tail (as assessed by surface replica electron microscopy). Selection of spermatozoa in vitro by allowing them to swim upwards through a nickel mesh also reduced the number of spermatozoa with abnormal morphology, particularly of the midpiece and tail. It is concluded that the apparent selection of morphologically normal spermatozoa is not a direct function of the female tract, but that the spermatozoa can effect their own selection because of their differential motility.
94 citations
TL;DR: A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-μL straws or 1.5-mL cryovials, with a decline in fertility explained by the loss of fertility of only one sperm, and only in large-volume conditions, probably due to the delay of use after thawing.
94 citations
Journal Article•
TL;DR: This review aims to explore the phenomenon of consistent variation in frozen semen quality between species and between individuals in an effort to find new insights into the reasons for cryoinjury.
Abstract: Although semen cryopreservation has been applied successfully in a few species, considerable variation in post-thaw semen viability exists. Independent of sperm quality before freezing, the semen of certain individuals will consistently freeze badly, resulting in poor motility, disrupted acrosome and plasma membrane, and thus reduced fertilising ability, indicating the existence of variation in membrane properties within species. A more comprehensive understanding of sperm cryobiology would be obtained by the investigation of within -species variation in the susceptibility of spermatozoa to cryoinjury. This review aims to explore the phenomenon of consistent variation in frozen semen quality between species and between individuals in an effort to find new insights into the reasons for cryoinjury. Recent studies suggest that there is a genetic basis for variation in postthaw semen quality, and argue that modern molecular technologies are able to identify markers linked to genes influencing this variation. The identification of genetic differences between individuals, which may be linked to cryosurvival, provides an opportunity to develop a functional and molecular understanding of the factors that influence semen cryopreservation, allowing selective breeding of desired traits and the development of genetic tests that predict the outcome of semen freezing.
94 citations