Topic
Semen
About: Semen is a research topic. Over the lifetime, 14571 publications have been published within this topic receiving 407739 citations. The topic is also known as: come & ejaculate.
Papers published on a yearly basis
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TL;DR: The belief that the predictive use of the results obtained in a standard boar semen quality analysis can reasonably be achieved by applying logistic correlation analyses among several function parameters of boar spermatozoa analysis and in vivo conception rates obtained after artificial insemination is supported.
151 citations
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TL;DR: The honeybee seminal fluid proteome represents an important addition to available data for comparative studies of seminal fluid peptides in insects and more similarity of the bee protein set with the current human seminal fluid protein set than with the known Drosophila seminal fluid proteins.
Abstract: Ejaculates contain sperm but also seminal fluid, which is increasingly recognized to be of central importance for reproductive success. However, a detailed biochemical composition and physiological understanding of seminal fluid is still elusive. We have used MS to identify the 57 most abundant proteins within the ejaculated seminal fluid of the honeybee Apis mellifera. Their amino acid sequences revealed the presence of diverse functional categories of enzymes, regulators and structural proteins. A number have known or predicted roles in maintaining sperm viability, protecting sperm from microbial infections or interacting with the physiology of the female. A range of putative glycoproteins or glycosylation enzymes were detected among the 57, subsequent fluorescent staining of glycolysation revealed several prominant glycoproteins in seminal fluid, while no glycoproteins were detected in sperm samples. Many of the abundant proteins that accumulate in the seminal fluid did not contain predictable tags for secretion for the cell. Comparison of the honeybee seminal fluid proteins with Drosophila seminal fluid proteins (including secreted accessory gland proteins known as ACPs), and with the human seminal fluid proteome revealed the bee protein set contains a range of newly identified seminal fluid proteins and we noted more similarity of the bee protein set with the current human seminal fluid protein set than with the known Drosophila seminal fluid proteins. The honeybee seminal fluid proteome thus represents an important addition to available data for comparative studies of seminal fluid proteomes in insects.
151 citations
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TL;DR: The validation of a sperm DNA fragmentation test based on the sperm chromatin dispersion test (SCD) for stallion spermatozoa and on its application to semen that was chilled or frozen-thawed is reported on.
150 citations
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TL;DR: A significant increase of superoxide anion was detected in the male partners of infertile couples and in some selected andrological conditions, and the toxic effect of super oxygen anion on sperm cells is dependent on the time of exposure and superoxideAnion concentration.
150 citations