Topic
Semen analysis
About: Semen analysis is a research topic. Over the lifetime, 4909 publications have been published within this topic receiving 143225 citations.
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TL;DR: Sperm quality is inversely correlated with lack of viability, DNA fragmentation, and ubiquitin fluorescence intensity means, however, none of the apoptotic markers correlate with ubiquit in labeling.
61 citations
TL;DR: Surgical correction of varicocele was associated with significant improvement in sperm morphology evaluated using Kruger classification, and concentration and count improved after varicocelectomy.
61 citations
TL;DR: Between 1980 and 1985, 6067 out-patient vasectomies were performed under local anaesthesia at the Elliot Smith Clinic in Oxford and 151 men were given a "special clearance", which sanctioned the discontinuation of other forms of contraception despite the persistence of scanty sperm in 2 consecutively examined semen samples at least 7 months after vasectomy.
Abstract: Between 1980 and 1985, 6067 out-patient vasectomies were performed under local anaesthesia at the Elliot Smith Clinic in Oxford. During this period 151 men (2.5%) were given a "special clearance". This sanctioned the discontinuation of other forms of contraception despite the persistence of scanty (less than 10,000/ml) sperm in 2 consecutively examined semen samples at least 7 months after vasectomy. These men have been reviewed and further specimens of semen requested after a minimum follow-up of 3 years (range 3-8); 50 patients supplied a specimen and all except 1 were azoospermic. No pregnancies attributable to failure of the vasectomy have been identified.
61 citations
TL;DR: Testicular volume, but not age or reproductive hormone levels, was indicative of successful semen collection, and adolescent boys with testicular volumes of more than 5 ml should be offered semen banking prior to gonadotoxic treatment or other procedures that could potentially damage future fertility.
Abstract: background: Cryopreservation of semen should be offered to adults before gonadotoxic treatment. However, the experience with semen collection in adolescents is still limited. The objective of this study was to evaluate potential correlates of successful semen sampling in adolescents. methods: A total of 86 boys (aged 12.2–17.9 years), referred for cryopreservation of semen prior to gonadotoxic treatment were included. Age, testicular volume, diagnosis and reproductive hormones were evaluated as correlates of successful semen collection. results: Median sperm concentration was 9.6 (range 0– 284) million/ml. Of the 86 included boys, 76 (88.4%) had spermatozoa in their ejaculate. Of the 76 patients for whom a semen sample was obtained, 71 (93.4%) had motile spermatozoa eligible for cryopreservation. Of the 86 boys, 74 produced a semen sample by masturbation, whereas semen samples were obtained from 12 patients by penile vibration or electroejaculation. The youngest patient with an ejaculate containing motile spermatozoa was 12.2 years old, and the smallest testicular volumes in boys associated with motile spermatozoa in the ejaculate were 6– 7 ml. Testicular volume correlated with sperm concentration (R ¼ 0.283, P ¼ 0.046), and the percentage of motile spermatozoa (R ¼ 0.410, P ¼ 0.003). Chronological age, but not reproductive hormones, also correlated with sperm concentration (R ¼ 0.25, P ¼ 0.049). conclusions: Semen was successfully collected and cryopreserved in 71 out of 86 boys and adolescents. Testicular volume, but not age or reproductive hormone levels, was indicative of successful semen collection. Regardless of their age, adolescent boys with testicular volumes of more than 5 ml should be offered semen banking prior to gonadotoxic treatment or other procedures that could potentially damage future fertility.
61 citations
TL;DR: To separate canine seminal plasma proteins (with SDS-PAGE) and to determine the correlation between specific proteins and semen characteristics, three ejaculates from 20 mixed-breed dogs, of unknown fertility, were collected by digital manipulation.
61 citations