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Semen analysis
About: Semen analysis is a research topic. Over the lifetime, 4909 publications have been published within this topic receiving 143225 citations.
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TL;DR: Saffron, as an antioxidant, is positively effective on sperm morphology and motility in infertile men, while it does not increase sperm count.
Abstract: Introduction: We conducted this study to determine the effects of saffron (Crocus sativus) on the results of semen analysis in men with idiopathic infertility. Materials and Methods: In this clinical trial, 52 nonsmoker infertile men whose problem could not be solved surgically were enrolled. They were treated by saffron for 3 months. Saffron, 50 mg, was solved in drinking milk and administered 3 times a week during the study course. Semen analysis was done before and after the treatment and the results were compared. Results: The mean percentage of sperm with normal morphology was 26.50 ± 6.44% before the treatment which increased to 33.90 ± 10.45%, thereafter ( P P P P 6 /mL at baseline and 44.92 ± 28.36A— 10 6 /mL after the treatment period ( P = .30). Conclusion: Saffron, as an antioxidant, is positively effective on sperm morphology and motility in infertile men, while it does not increase sperm count. We believe further studies on larger sample sizes are needed to elucidate the potential role and mechanism of action of saffron and its ingredient in the treatment of male infertility.
58 citations
TL;DR: To improve the results obtained with a reference cryopreservation extender for freezing caprine semen, glutamine was added to 18 split ejaculates at concentrations of 0, 20, 40, 80 and 120 mM and significantly improved sperm motility compared with the control extender.
Abstract: To improve the results obtained with a reference cryopreservation extender (control extender: Triladyl + 20% (v/v) egg yolk + 6.4% (v/v) glycerol) for freezing caprine semen, glutamine was added to 18 split ejaculates at concentrations of 0, 20, 40, 80 and 120 mM (experiment 1). In experiment 2, glutamine was added to 18 split ejaculates at concentrations of 20, 25, 30, 35 and 40 mM. In the third experiment, the egg yolk was replaced with the low-density lipoprotein (LDL) fraction of egg yolk. The quality of frozen then thawed spermatozoa in each extender was compared using computer-assisted semen analysis. In experiment 1, glutamine at concentrations of 20 mm and 40 mm significantly improved sperm motility compared with the control extender. However, at 120 mM, a significant decrease in motility and velocity was observed. In experiment 2, motility, curvilinear velocity and amplitude of lateral head displacement (ALH) were improved in glutamine at 25 mM compared with the control. In experiment 3, 8% LDL and 25 mM glutamine significantly improved sperm motility, straight line velocity and ALH. In the fourth experiment, the quality of the previously defined freezing extender (Triladyl + 8% (v/v) LDL + 25 mM glutamine + 6.4% (v/v) glycerol) was tested by comparing acrosome, tail membrane, plasma membrane and DNA integrity in 18 split ejaculates of frozen then thawed spermatozoa with spermatozoa that had been frozen then thawed in the control extender, and with spermatozoa from fresh, unfrozen sperm. The percentage of spermatozoa with intact acrosomes and tail membranes was significantly higher with the newly defined extender than that observed with the control extender. There was no significant difference in the percentage of spermatozoa with intact DNA between the frozen and fresh semen.
58 citations
TL;DR: PM2.5 exposure leads to oxidative stress impairment via PI3K/Akt signaling pathway on male reproduction in rats, with noticeable decreased fertility, significantly reduced sperm count, increased sperm abnormality rate and severe testicular damage in histomorphology.
58 citations
TL;DR: In ejaculated semen, the percent sperm apoptosis was associated with several measures of semen quality and inverse associations between percent apoptosis and sperm motility and progressive motility, and morphology with a normal or good pattern of fertilization by Kruger strict criteria.
Abstract: This work was undertaken to explore the associationbetween human semen characteristics and apoptosis in ejaculatedsperm. We collected semen samples from 23 consecutive male pa-tients who presented to the Andrology Laboratory at MassachusettsGeneral Hospital (MGH) for routine semen analysis. Sperm concen-tration and motility were measured using computer-assisted spermanalysis. Morphology was assessed using Tygerberg strict criteria.The DNA diffusion assay was used to assess the percentage ofapoptosis in ejaculated sperm. In this assay, cells were mixed withagarose and placed into a microgel on a microscopic slide. The cellswere stained with YOYO-1 dye, and apoptotic cells were viewedunder a fluorescent microscope. Among 23 men, the mean (SD)sperm concentration, percent motility, percent progressive motility,and normal morphology were 125.5 (92.3) million/mL, 45.6% (22.2),28.4% (15.2), and 8.0 (4.6), respectively. The mean (SD) percent ofapoptosis in ejaculated sperm was 8.3% (6.2) with a range from1.1% to 20.1%. There were inverse associations between percentapoptosis and sperm motility (P5 .0025), progressive motility (P5.0051), and morphology with a normal or good pattern of fertilizationby Kruger strict criteria (P5 .0045), and a positive relationship be-tween percent apoptosis and sperm tail defects (P5 .0053). In ejac-ulated semen, the percent sperm apoptosis was associated withseveral measures of semen quality.Key words: Semen quality, sperm motility, sperm morphology,DNA diffusion assay.J Androl 2006;27:112–120
58 citations
TL;DR: Recent findings regarding the capacitation‐related proteome of spermatozoa are outlined, and how these proteins may be utilized to better understand the fertility of domestic animals are discussed.
Abstract: Infertility and subfertility account for huge economic losses in the animal industry; indeed, 50% of animal breeding failure is associated with male infertility. Approximately 70% of cattle and 90% of pig livestock are currently produced by artificial insemination. Therefore, breeding-male selection is extremely important for the genetic benefits of progeny. Although conventional semen analysis provides an initial measure of male fertility, its clinical value is questionable. Proteomics approaches recently identified candidate protein markers in spermatozoa for evaluating male fertility. Fertility-related proteins in capacitated boar spermatozoa were shown to predict boar fertility more precisely then those detected in ejaculated spermatozoa, which motivated the development of more accurate and sensitive tools for the assessment of male fertility in relation to sperm function and fertilization. Although protein markers in spermatozoa are capable of discriminating fertile and infertile males, clinical trials are required to validate their predictive utility. This review outlines recent findings regarding the capacitation-related proteome of spermatozoa, and discusses how these proteins may be utilized to better understand the fertility of domestic animals.
58 citations