Semen analysis

About: Semen analysis is a research topic. Over the lifetime, 4909 publications have been published within this topic receiving 143225 citations.

Reproductive status in long-term bone marrow transplant survivors receiving busulfan-cyclophosphamide (120 mg/kg)

Abstract: There are few published data on the recovery of fertility after 'little' Bu-Cy (busulfan 16 mg/kg, cyclophosphamide 120 mg/kg) conditioning for BMT. To address this, we identified 19 females aged less than 40 years at transplant and 47 males from a single centre who were alive a minimum of 2 years after BMT with little Bu-Cy as conditioning and who were evaluable for testing. FSH, LH, testosterone and inhibin B levels were measured in males. Twenty-six also had semen analysis, a median of 5 years post transplant; 21 had detectable sperm, with 11 having counts >20 x 10(6)/ml. There was an association between prolonged chronic graft-versus-host disease and low sperm counts. FSH and inhibin B levels correlated with sperm counts but not to the extent that they could reliably predict counts in individual patients. An additional six of seven males attempting to father children did so, a median of 3.2 years post transplant. Low testosterone levels were noted in 12% of males, most of whom had symptoms consistent with androgen deficiency. FSH, LH and oestradiol levels in the absence of hormone replacement therapy were measured in females; all remained amenorrheic with endocrine evidence of ovarian failure. These results have implications for fertility counselling and hormone replacement therapy both pre- and post BMT.

Meiotic studies in a series of 1100 infertile and sterile males

Abstract: Meiotic studies have been carried out in a series of 1100 infertile and sterile males. Of these, 599 cases have been studies in testicular biopsy, and 501, in semen samples. This is the largest meiotic series published so far. The incidence of meiotic anomalies was 4.3%. The most frequent chromosome abnormality was desynapsis (3.7%). However, the number of cases with a meiotic arest, usually due (73.9%) to synaptic anomalies in prophase I, was much higher (18.4%). An attempt is made to correlate the incidence of meiotic anomalies with the results of semen analysis. We discuss the prognosis of desynapsis, based on 41 cases studied, and reevaluate the results obtained in semen samples as compared with our previous results.

Evidence for regional differences of semen quality among fertile French men. Fédération Francaise des Centres d'Etude et de Conservation des Oeufs et du Sperme humains.

Abstract: The world literature on human semen quality indicates apparent geographical differences but these might primarily depend on variations among studies for subject recruitment strategy, semen analysis or data processing methods. A retrospective analysis on the quality of semen from 4710 healthy unselected fertile men, who were candidate semen donors to sperm banks in university hospitals in eight different French areas during the period 1973-1993, was undertaken. In these centres, all the men were referred under the same guidelines and all semen samples were analysed using similar methodologies. Significant differences were found between centres for seminal volume, sperm concentration, total number of spermatozoa in the ejaculate and percentage of motile spermatozoa (all P < 0.0001). Multiple regression analysis accounting for the age, sexual abstinence before semen collection and year of semen collection also showed regional differences: compared to Paris, the seminal volume was higher in Caen (P < 0.001) and lower in Toulouse (P < 0.01), the total number of spermatozoa was higher in Lille (P < 0.001) and lower in Toulouse (P < 0.05) and the percentage of motile spermatozoa was higher in Bordeaux and lower in Tours (both P < 0.001). This is the first study providing evidence for regional differences in the human semen quality.