Topic
Semen analysis
About: Semen analysis is a research topic. Over the lifetime, 4909 publications have been published within this topic receiving 143225 citations.
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TL;DR: The results indicate that DNA damage is significantly correlated to SSP as well as age and is most severe in patients with oligoasthenoteratozoospermia, which may be responsible for the strong relationship between SSP and DNA fragmentation index.
92 citations
TL;DR: Three-layer Puresperm gradients with large semen loading volumes with large sperm concentrations, percentage motility, percentage vitality and percentage normal morphology appear to be an attractive alternative for sperm separation in medically assisted conception.
Abstract: To evaluate and optimize the sperm separation efficiency of a novel silane-coated silica bead (Puresperm), serial studies were carried out to compare the various sperm parameters between: (i) three-layer (90%-70%-40%) Puresperm and three-layer (90%-70%-40%) conventional polyvinylpyrrolidone (PVP)-coated silica bead (Percoll) gradients; (ii) three-layer (90%-70%-40%) and two-layer (90%-45%) Puresperm gradients and separately the same for Percoll; and (iii) large (3.0 ml) and small (0.75 ml) semen loading volumes on three-layer Puresperm gradients. Normozoospermic semen samples were treated and analysed in 12 replicates for each experiment. Manual evaluation of concentration, percentage motility, percentage vitality, percentage normal morphology; computer-assisted semen analysis evaluation of concentration, percentage motility, grade of motility, motion characteristics (curvilinear velocity, linearity, amplitude of lateral head velocity, beat cross frequency, percentage hyperactivation); and yields from the initial semen samples were compared. Percoll was found to be superior to Puresperm in concentration, percentage motility, percentage vitality and yields after three-layer density gradient centrifugation. There were no significant differences in sperm parameters between two- and three-layer Percoll gradients, but three-layer Puresperm gradients behaved significantly better than two-layer gradients. Large semen volume loads on three-layer Puresperm gradients resulted in greater sperm concentrations, percentage motility, percentage vitality and percentage normal morphology, but small semen volume loads produced greater yields of good-quality spermatozoa. In the light of Percoll being withdrawn from the shelf for the use of assisted reproduction because of the presence of PVP, three-layer Puresperm gradients with large semen loading volumes appear to be an attractive alternative for sperm separation in medically assisted conception.
92 citations
TL;DR: Results do not support the hypothesis that polyandry evolved in response to costs associated with mating with old males in the guppy, by investigating whether age affects post‐copulatory male traits and sperm competition success.
Abstract: Deleterious mutations can accumulate in the germline with age, decreasing the genetic quality of sperm and imposing a cost on female fitness. If these mutations also affect sperm competition ability or sperm production, then females will benefit from polyandry as it incites sperm competition and, consequently, minimizes the mutational load in the offspring. We tested this hypothesis in the guppy (Poecilia reticulata), a species characterized by polyandry and intense sperm competition, by investigating whether age affects post-copulatory male traits and sperm competition success. Females did not discriminate between old and young males in a mate choice experiment. While old males produced longer and slower sperm with larger reserves of strippable sperm, compared to young males, artificial insemination did not reveal any effect of age on sperm competition success. Altogether, these results do not support the hypothesis that polyandry evolved in response to costs associated with mating with old males in the guppy.
91 citations
TL;DR: Increased BMI was associated with decreased semen quality, affecting volume, concentration, and motility, and Multivariate models including age and abstinence duration confirmed these results.
91 citations
TL;DR: In this article, the authors evaluated twenty male marathon athletes by hormonal profiles, psychologic testing, anthropomorphic indices, and semen evaluations, and concluded that vigorous endurance training may be associated with significantly decreased T values but not sperm production.
91 citations