scispace - formally typeset
Search or ask a question

Showing papers on "Serum albumin published in 1970"


Journal ArticleDOI
TL;DR: The Golgi fraction from rat liver appears to have a unique and characteristic protein profile after electrophoresis in polyacrylamide gels as compared with endoplasmic reticulum, plasma membranes and mitochondria.

152 citations



Journal ArticleDOI
TL;DR: Nonsteroidal anti-inflammatory agents inhibited the heat denaturation of serum albumin at drug levels betow 1 mM, as measured by turbidity and viscosity changes.

132 citations


Journal ArticleDOI
TL;DR: Radioactive μspheres have been prepared from human serum albumin (HSA) in sizes between 12 and 44 &μ; and they have advantages over other particles that are now being used for studies of the circulation.

119 citations


Journal ArticleDOI
TL;DR: The effects of oral contraceptives on the concentrations of plasma proteins, although less marked, generally paralleled those produced by late pregnancy, an observation consistent with the significant influences which sex hormones exert on various metabolic activities of this organ.

105 citations


Journal ArticleDOI
TL;DR: The binding characteristics and absorption spectra of the human and bovine albumin-bilirubin complexes in the presence and absence of oleate or salicylate were very similar, but the effects generated by the addition of bilirubsin to the human albumin were very different from those obtained with the bovines.

104 citations


Journal ArticleDOI
TL;DR: The results show that plasma LDL peptide does enter the inner arterial wall, presumably across the intima, and that the Intima may be the site of a slowly exchanging LDL pool.

100 citations


Journal ArticleDOI
TL;DR: It was found that the mitochondrial lipids rather than the proteins were involved in the binding, suggesting that the binding of bilirubin with lipid alters the characteristics of mitochondrial membranes so that membrane-linked functions are impaired.

99 citations


Journal ArticleDOI
TL;DR: The results of time-course experiments of the incorporation of [14C]leucine in vivo into the serum albumin fraction, which was prepared from the “nascent protein” fractions of both kinds of polysomes by the immunological methods, showed that the labelledalbumin fraction was located only on boundpolysomes and supported the indication of exclusive synthesis of this protein by bound polysome.

96 citations


Journal ArticleDOI
TL;DR: It is suggested that transferrin and α 2 -macroglobulin may have an important role in internal zinc exchange and when serum zinc falls to very low levels, it may account for nearly one-half of the total serum zinc.
Abstract: The distribution of 65 Zn among the serum proteins of 18 cirrhotic patients and 10 normal individuals was studied. Total zinc was measured in each serum. Zinc was bound to albumin, transferrin and α 2 -macroglobulin in all subjects. It was bound to IgG in all cirrhotics and five of ten normals. Utilizing the percentage distribution of 65 Zn and total serum zinc, the amount bound in each fraction was calculated. Albumin-bound zinc correlated well with total serum zinc (r = 0.97). Total serum zinc was lower in cirrhotics than in normals (p α 2 -macroglobulin. The total amount bound to these proteins (Tf- α 2 M) was of remarkably constant and equivalent size in normals and cirrhotics. Transferrin concentration was significantly lower (p α 2 -macroglobulin was higher in cirrhotics although the difference was not significant (p α 2 -macroglobulin. However, the total amount bound to Tf- α 2 M remains remarkably constant even when total serum zinc falls as low as 12 μg./ml. The constancy of the amount of zinc bound to Tf- α 2 M implies metabolic control of this fraction. When serum zinc falls to very low levels, it may account for nearly one-half of the total serum zinc. It is suggested that transferrin and α 2 -macroglobulin may have an important role in internal zinc exchange.

95 citations



Journal ArticleDOI
TL;DR: The presence of staining in the brain reflects the presence of increased amounts of albumin either at the time of observation or at some time in the past, conceptually related to the blood-brain barrier.
Abstract: Vital staining was studied by quantitating the amounts of Evans blue and serum albumin in muscle and in abnormal brain. On the basis of differences in the ratio of dye to albumin in tissue and in serum, it was concluded that the coloration of tissue involves both the presence of serum albumin in tissue and the binding of dye not associated with serum albumin by tissue. The mode of binding of free dye by edematous cerebral tissue was studied by comparing the chemical structures of a series of acid dyes which did and did not function as vital stains. It was concluded that substantivity for edematous cerebral tissue requires the presence on the dye molecule of paired widely separated electron-donating hydrogen bond forming groups placed away from the solubilizing acid groups. It is suggested that the primary mode of binding for Evans blue in tissue is through the formation of hydrogen bonds between the azo groups of the dye and the hydroxy groups of tissue polysaccharides. These findings are conceptually related to the blood-brain barrier in that the presence of staining in the brain reflects the presence of increased amounts of albumin either at the time of observation or at some time in the past.


Journal ArticleDOI
TL;DR: Phenobarbital treatment of the rats in vivo stimulates the rate of [14C]leucine incorporation into membranous proteins and increases the number of microsomal membranes, but has little or no effect on the microSomal albumin content or its synthesis.

Journal ArticleDOI
TL;DR: Aspiryl-chloride was conjugated with human serum albumin, human gamma globulin, rabbit gamma Globulin, and poly- l -lysine, and no evidence of specific immunologic reactions was found.

Journal ArticleDOI
TL;DR: Rheumatoid patients with a raised alkaline phosphatase with a control group of rheumatoids patients matched for age and sex in whom the alkalineosphatase was normal are compared to detect evidence of liver dysfunction or bone disease.


Journal ArticleDOI
TL;DR: Decomposition of bilirubin and biliverdin under intense fluorescent light was studied in vitro at p H levels ranging from 7.4 to 13.0 and it was shown that the process of photodecay proceeded from bilirubs to biliversdin to weakly diazo-reactive derivatives to highly polar, diozo-negative products.

Journal ArticleDOI
TL;DR: Differences in the character of the released label, in the rate of release of the label, and in the influence of the reincubation serum all indicate separate pathways for the renewal of phosphatide introduced into red cells by these two routes.
Abstract: To study the catabolism of erythrocyte phospholipids, human erythrocytes were labeled with radioactive fatty acid (FA). Labeling was performed by the two separate routes which together are thought to be responsible for the majority of phosphatide renewal in the red cell: (a) passive equilibration of erythrocytes with preformed acid-labeled red cell phosphatidylcholine (PC) and (b) active, "acylase"-dependent, incorporation of free fatty acid in the presence of ATP coenzyme A and magnesium. (As measured here "acylase" = the over-all effect of fatty acid thioesterification and the action of acyl-CoA: acylglycerophosphoryl acyltransferase.) The labeled cells were then reincubated in serum and the loss of radioactivity from cells into serum was examined. The phosphatide fatty acid introduced by these two routes was incorporated into two distinct cellular phosphatide pools with little mixing. The fatty acid in passively introduced phosphatidyl choline was released primarily as the intact phosphatide, whereas actively incorporated PC fatty acid was released primarily as free FA. As expected for an exchange process, the passively introduced PC was released at the same rate as it was introduced. However, the actively incorporated FA was released from the cell at a slower rate than its incorporation into PC. This latter phenomenon was explained by the observation of transfer of PC fatty acid to phosphatidyl ethanolamine and "neutral lipid" before its release to serum. This transfer process was inhibited by previous heating of the reincubation serum. The differences in the character of the released label, in the rate of release of the label, and in the influence of the reincubation serum all indicate separate pathways for the renewal of phosphatide introduced into red cells by these two routes.



Journal ArticleDOI
TL;DR: The ease of application and merits of the method were demonstrated by a comprehensive study of the influence of pH on the binding of 8‐nitrotheophylline by bovine serum albumin and the study of competitive inhibition of protein binding.

Journal ArticleDOI
TL;DR: This article showed that 30min exposure to more than 5 ppM 0/sub 3, 70 ppM NO/sub 2/ or 330 ppM SO/sub 4/ increased sensitization with albumin via the respiratory airway in guinea pigs.
Abstract: Repeated 30-min exposures to more than 5 ppM 0/sub 3/, 70 ppM NO/sub 2/ or 330 ppM SO/sub 2/ enhanced sensitization with albumin via the respiratory airway in guinea pigs. The effect was local, as intraperitoneal introduction of albumin antigens had no effect. The effect may have been due to cessation of transport activities (longer residence) or change in epithelial permeability facilitating entry of antigens.

Journal ArticleDOI
TL;DR: It is suggested that the formation of an apolar complex mediated through an initial electrostatic attraction gives rise to the changes in permeability properties of the model membrane which were studied previously.

Journal ArticleDOI
TL;DR: Serum-levels of four plasma-proteins, assayed by radial immunodiffusion, have been compared in late pregnancy and in age-matched non-pregnant female controls, and in apparently healthy women before and after taking oral contraceptives, and the effect of female sex hormones on IgG may contribute to the predominance of certain immunological disorders in females.

Journal ArticleDOI
TL;DR: Phenylbutazone, flufenamic acid and salicylate at therapeutic concentrations, which stabilize some proteins in whole serum, stabilized also canine erythrocytes against heat in a solution containing 3% bovine albumin.



Journal ArticleDOI
TL;DR: The view is strongly supported that, while small amounts of IgA do pass from serum to saliva, this accounts for no more than a small percentage of the total IgA in saliva, and the IgA found in the saliva probably enters by nonspecific transudation common to all serum proteins.

Journal ArticleDOI
TL;DR: It was found that they react together to form intermediate products which then break down to form osmium black, and glutaraldehyde was revealed as the most effective crosslinking agent by gel filtration and electrophoresis.
Abstract: The reactions between osmium tetroxide and glutaraldehyde and formaldehyde were investigated. It was found that they react together to form intermediate products which then break down to form osmium black. Glutaraldehyde reacts much more rapidly with osmium tetroxide than formaldehyde. The rates of the reactions are increased by increasing the glutaraldehyde concentration or adding bovine serum albumin to the reaction mixture. The reaction rates increase with temperature. The mixtures of fixatives were also tried on tissues and the results paralleled the model experiments. The crosslinking of bovine serum albumin by osmium tetroxide, formaldehyde and glutaraldehyde singly and in mixtures was quantitatively assessed by viscosimetry, gel filtration and disc electrophoresis coupled with densitometry. The crosslinking of bovine serum albumin by pairs of fixatives was less than that produced by the most effective of the pair. After 5 min reaction osmium tetroxide was the most effective crosslinking agent according to viscosimetric experiments, but after one hour's reaction with bovine serum albumin, glutaraldehyde was revealed as the most effective crosslinking agent by gel filtration and electrophoresis.