Topic
Serum albumin
About: Serum albumin is a research topic. Over the lifetime, 16337 publications have been published within this topic receiving 516395 citations. The topic is also known as: blood albumin & ANALBA.
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TL;DR: This paper focuses on human serum albumin (HSA), the most abundant plasma protein, proposed to serve antioxidant functions in the vascular compartment, supporting a role of HSA-Cys34 as an important redox regulator in extracellular compartments.
Abstract: Sulfenic acid (RSOH) is a central intermediate in both the reversible and irreversible redox modulation by reactive species of an increasing number of proteins involved in signal transduction and enzymatic pathways. In this paper we focus on human serum albumin (HSA), the most abundant plasma protein, proposed to serve antioxidant functions in the vascular compartment. Sulfenic acid in HSA has been previously detected using different methods after oxidation of its single free thiol Cys34 through one- or two-electron mechanisms. Since recent evidence suggests that sulfenic acid in HSA is stabilized within the protein environment, this derivative represents an appropriate model to examine protein sulfenic acid biochemistry, structure and reactivity. Sulfenic acid in HSA could be involved in mixed disufide formation, supporting a role of HSA-Cys34 as an important redox regulator in extracellular compartments.
126 citations
01 Jul 2011
TL;DR: The SVM model was integrated to a free, web-based prediction platform and the potential offered by the combined use of in silico calculation methods and experimental binding data is illustrated.
Abstract: Motivation: Human serum albumin (HSA), the most abundant plasma protein is well known for its extraordinary binding capacity for both endogenous and exogenous substances, including a wide range of drugs. Interaction with the two principal binding sites of HSA in subdomain IIA (site 1) and in subdomain IIIA (site 2) controls the free, active concentration of a drug, provides a reservoir for a long duration of action and ultimately affects the ADME (absorption, distribution, metabolism, and excretion) profile. Due to the continuous demand to investigate HSA binding properties of novel drugs, drug candidates and drug-like compounds, a support vector machine (SVM) model was developed that efficiently predicts albumin binding. Our SVM model was integrated to a free, web-based prediction platform ( http://albumin.althotas.com). Automated molecular docking calculations for prediction of complex geometry are also integrated into the web service. The platform enables the users (i) to predict if albumin binds the query ligand, (ii) to determine the probable ligand binding site (site 1 or site 2), (iii) to select the albumin X-ray structure which is complexed with the most similar ligand and (iv) to calculate complex geometry using molecular docking calculations. Our SVM model and the potential offered by the combined use of in silico calculation methods and experimental binding data is illustrated.
Contact: eszter.hazai@virtuadrug.com
Supplementary information:Supplementary data are available at Bioinformatics online.
126 citations
TL;DR: Serum albumin merely reflected the presence of a systemic disease, which was the most important risk factor for patient survival, and the results confirm the strength of serum albumin as predictor of survival.
Abstract: ObjectiveTo analyze the effect of serum albumin using immunoturbidimetry, demographic, biochemical, and kinetic factors on survival of continuous ambulatory peritoneal dialysis (CAPD) patients.Desi...
126 citations
TL;DR: While studying the interactions of the vaccinia virus complement control protein (VCP) with complement, non-specific binding of VCP to BSA is found and the need to perform critical controls to ensure that ELISA reactants do not inappropriately bind to the blocking agent is highlighted.
126 citations
TL;DR: The data suggest that the hemin-LDL complex may exist in vivo and that its oxidative potential should be considered pro-atherogenic.
126 citations