Topic
Serum albumin
About: Serum albumin is a research topic. Over the lifetime, 16337 publications have been published within this topic receiving 516395 citations. The topic is also known as: blood albumin & ANALBA.
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TL;DR: In this article, the binding properties of human serum albumin via purely conformational changes are investigated. But no gross alteration can be observed in the electrophoretic and chromatographic patterns of albumin, whereas localized modifications are indicated by the changes in absorption and fluorescence spectra and in polarization degree.
110 citations
TL;DR: Interaction of lipids and proteins with mucus glycoprotein contributes significantly to the viscosity of gastric mucus, and this contribution is proportional to albumin concentrationup to 10%, and to IgA concentration up to 5%.
110 citations
TL;DR: The simple PCR method allows robust PCR independent of template purity or amplification length and enabled long PCR over 20 kb when Taq DNA polymerase with 3′‐5′ exnuclease activity was used for the amplification.
Abstract: The effects of bovine serum albumin, dithiothreitol, and glycerol on PCR were studied. PCR under standard conditions failed the amplification of an enterohemorrhagic E. coli DNA fragment when the boiled bacterial cell lysate was used as the template. The addition of either one of bovine serum albumin, dithiothreitol, or glycerol in the reaction mixture allowed the specific fragment amplification; and the optimum concentrations were as follows: bovine serum albumin, 1 mg/ml; dithiothreitol, 10 mM; and glycerol, 5%. In addition, when all of the three agents were included at the above concentrations, the PCR yield was further increased. The effect of the three-agent mixture was not the template specific. Furthermore, the mixture enabled long PCR over 20 kb when Taq DNA polymerase with 3'-5' exonuclease activity was used for the amplification. Our simple PCR method allows robust PCR independent of template purity or amplification length.
110 citations
Patent•
25 Oct 1988TL;DR: In this article, the use of mature human serum albumin residues 1 to n, where n is between 369 and 419 inclusive, are useful as substitutes for albumin in the treatment of burns and shock in humans, the clearances of undesirable compounds, such as bilirubin from human blood, in laboratory growth media and in HSA assays.
Abstract: Polypeptides corresponding to mature human serum albumin residues 1 to n, where n is between 369 and 419 inclusive, are useful as substitutes for albumin in the treatment of burns and shock in humans, the clearances of undesirable compounds, (such as bilirubin) from human blood, in laboratory growth media and in HSA assays. HSA (1-389) is particularly preferred, although not novel per se. The polypeptides may be produced by recombinant DNA techniques, especially in yeast.
110 citations
TL;DR: In this article, the influence of albumin-bound thyroxin (T4) on apparent free T4 values obtained by two "unbound analog" free t4 methods (AmerlexR Free T4 and Clinical Assays one-step Free t4) was assessed.
Abstract: We have assessed the influence of albumin-bound thyroxin (T4) on apparent free T4 values obtained by two "unbound analog" free T4 methods (AmerlexR Free T4 and Clinical Assays one-step Free T4). We evaluated sera showing three different albumin anomalies: total hereditary analbuminemia, partially corrected analbuminemia, and familial dysalbuminemic hyperthyroxinemia, where abnormal albumin-binding of analog tracer is associated with high apparent free T4 values by these methods. In hereditary analbuminemia, free T4 was almost undetectable by both assays; in contrast, free T4 by equilibrium dialysis was normal. After addition of T4-free human serum albumin, the apparent free T4 concentration in total hereditary analbuminemia became normal by the analog methods. Immunoprecipitation of [125I]T4 and the unidentified labeled kit analogs by antiserum to human albumin was negligible in untreated total hereditary analbuminemia and approximately twice normal in familial dysalbuminemic hyperthyroxinemia. Therefore, alterations in tracer binding to albumin correlate with the apparent free T4 concentrations obtained by the analog methods. The interactions of the unidentified analog tracers and T4 with albumin are such that these techniques principally reflect the albumin-bound T4 moiety.
110 citations