Topic
Sister chromatid exchange
About: Sister chromatid exchange is a research topic. Over the lifetime, 3187 publications have been published within this topic receiving 90029 citations. The topic is also known as: replication-born DSB repair by SCE & GO:1990414.
Papers published on a yearly basis
Papers
More filters
••
TL;DR: A preliminary study indicates the possibility of using SCE as a preclinical marker in cervical cancer cases and the values of cancer cases deviate significantly from that of controls.
Abstract: The frequency of sister chromatid exchange (SCE) was investigated in 13 women with cervical cancer together with 11 control women. The SCE frequencies were found to be 10.05±2.35 and 6.95±1.53 in cancer cases and controls, respectively. The SCE values of cancer cases deviate significantly from that of controls. The SCE in chromosome groups E, F, and G was found to be more in comparison to controls (P<0.001). This preliminary study indicates the possibility of using SCE as a preclinical marker.
34 citations
••
TL;DR: It was found that estradiol-17beta itself and possibly its metabolites are potent mutagens beyond a particular dose in human lymphocytes.
Abstract: The cytogenetic effect of a hormonal steroid, estradiol-17β, was assessed in peripheral blood human lymphocyte culture. Sister chromatid exchanges (SCE) and chromosome aberrations (CA) were scored as genetic end points. Significant induction of CA was observed at 25 μg/ml and 50 μg/ml concentrations of estradiol-17β in the absence of microsomal activation. The drug was effective in all treatments in the presence of rat liver S9 microsomal fraction (S9 mix) and exhibited increased frequency of chromosomal aberrations. The drug was effective in increasing the SCE frequency which was found to be maximum at the dose of 50 μg/ml concentration (i.e., 4.34±1.22) both with and without metabolic activation. It was found that estradiol-17β itself and possibly its metabolites are potent mutagens beyond a particular dose in human lymphocytes.
34 citations
••
TL;DR: Sister chromatid exchange points were counted in lymphocytes in peripheral blood drawn from hospital personnel exposed to anaesthetics as well as from persons not exposed.
Abstract: Sister chromatid exchanges (SCE) and sister chromatid exchange points (SCE-points) were counted in lymphocytes in peripheral blood drawn from hospital personnel exposed to anaesthetics as well as from persons not exposed.
A total of 38 healthy persons were investigated, representing female nurse anaesthetists, male physicians practising anaesthesia, female nurses from the intensive care unit, and female secretaries.
The mean SCE number per cell for each person was used as the variable, and the Mann-Whitney U-test was applied to test for differences between groups. The group of secretaries seemed to differ from the other three groups, which appeared identical (P<0.001). Correlation of cigarette smoking and number of SCE could not be demonstrated (r=.255, n = 38).
It was concluded that by this method there was no indication of a mutagen effect of long-term exposure to waste anaesthetic gases such as halothane and nitrous oxide.
34 citations
••
TL;DR: It is suggested that a genotoxic effect due to inorganic lead may occur in long-term lead-exposed men and that this effect could be related to a serious disease for which he was undergoing treatment.
34 citations
••
TL;DR: SCE induction by 3AB in the second cycle is not dependent on the presence of BrdU in template DNA, suggesting that an imbalance in the deoxycytidine precursor pool did not account for the effect.
Abstract: The poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide (3AB), significantly increases sister chromatid exchange (SCE) frequency without causing apparent damage to cellular DNA. A previous study has suggested that the increase of SCEs by 3AB results from DNA replication on a template strand containing bromodeoxyuridine (BrdU), which is used to visualize SCEs. Therefore, to study the importance of BrdU incorporation on the induction of SCEs by 3AB, we analyzed exchanges induced during the first round of replication (twin SCEs) and those induced during the second (single SCEs). 3AB increased the formation of SCEs in both replication cycles, but significantly more exchanges were induced in the second cycle, when BrdU was present in the template DNA. These data are consistent with the suggestion that the presence of BrdU in the template strand of DNA plays an important role in SCE induction by 3AB. However, we also studied 3AB-induced SCEs by autoradiography of cells cultured with 3H-thymidine (3H-dT) instead of BrdU. A significant increase in SCE frequency was also observed in cells from these cultures. Furthermore, the analysis of twin and single SCEs showed that with 3H-dT too, there was a greater increase in SCEs in the second cycle than in the first. Thus, SCE induction by 3AB in the second cycle is not dependent on the presence of BrdU in template DNA. Incubation of cells with deoxycytidine was found to have no effect on the frequency of SCEs induced by 3AB, suggesting that an imbalance in the deoxycytidine precursor pool did not account for the effect.
34 citations