Topic
Sister chromatid exchange
About: Sister chromatid exchange is a research topic. Over the lifetime, 3187 publications have been published within this topic receiving 90029 citations. The topic is also known as: replication-born DSB repair by SCE & GO:1990414.
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TL;DR: The timing of meiosis and the segregation of tritium-labeled chromosomes has been studied in Ornithogalum virens.
Abstract: The timing of meiosis and the segregation of tritium-labeled chromosomes has been studied in Ornithogalum virens. Tritiated thymidine was administered to the inflorescences by a wick feeding process. The length of time required for labeled cells to pass from premeiotic DNA synthesis to the tetrad stage of meiosis is approximately 4 days. The labeled chromosomes display a semiconservative segregation pattern when labeled one division prior to meiosis; however, chromatid exchange events occur prior to, or during meiosis, or both. These exchange events are most likely a combination of crossing over between homologous chromosomes and sister chromatid exchange. The reductional separation of the centromere region at the first meiotic division is confirmed. The observations do not support Sax's two plane theory of crossing over, but are consistent with the theory that chiasmata are the cytological consequences of genetic exchange.
27 citations
TL;DR: Results indicate that fenvalerate in the presence of S9-mix is able significantly to increase the frequency of CA, while in the SCE test this increase occurred both in the Presence and in the absence of a rat liver activation system.
Abstract: A synthetic pyrethroid insecticide, fenvalerate, was tested for its ability to induce chromosome aberrations (CA) and sister chromatid exchanges (SCE) in CHO cells. Fenvalerate was assayed both in the presence and in the absence of a rat liver activation system (S9-mix).
Our results indicate that fenvalerate in the presence of S9-mix is able significantly to increase the frequency of CA, while in the SCE test this increase occurred both in the presence and in the absence of S9-mix.
In addition, fenvalerate affected the cell cycle, causing a decrease in the mitotic index (MI) and in the proliferative rate index (PRI). © 1992 Wiley-Liss, Inc.
27 citations
TL;DR: Of the tests used, SCE was found to be the most sensitive endpoint for indicating a biological response and since methods for restricting the MN analysis to only cells at risk were not used, this statement requires verification.
27 citations
TL;DR: The results strongly suggest that in BrdUrd-substituted cells, the duration of the S phase and therefore the rate of DNA replicationfork displacement provoked by lowering the culture temperature and/or the intracellular oxygen concentration plays a major role in the formation of SCEs.
27 citations
Journal Article•
TL;DR: The SCE-inducing activities of Epstein-Barr virus-transformed human lymphoblastoid cell lines were well correlated with their mutagenic activities assayed with the Salmonella system by Ames' and Sugimura's groups, although there were a few but significant deviations.
Abstract: Epstein-Barr virus-transformed human lymphoblastoid cell lines are suitable for detection of sister chromatid exchange (SCE) induced by mutagens-carcinogens because they have shown a stable chromosome number and stable frequency of spontaneous SCE for more than two years in culture. Their spontaneous and induced SCE frequencies were practically the same as those of phytohemagglutinin-stimulated lymphocytes from the same blood donors. The SCE responses of one established cell line, NL3, to 13 typical mutagens and five nonmutagens were examined. This cell line responded to all the mutagens tested but not to the nonmutagens. The SCE-inducing activities of these chemicals were well correlated with their mutagenic activities assayed with the Salmonella system by Ames9 and Sugimura9s groups, although there were a few but significant deviations.
27 citations