scispace - formally typeset
Search or ask a question
Topic

Small hairpin RNA

About: Small hairpin RNA is a research topic. Over the lifetime, 9279 publications have been published within this topic receiving 285471 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: Results indicate that miR-122 and its target gene NOD2 may play an important role in the injury of intestinal epithelial cells induced by LPS.

86 citations

Journal ArticleDOI
TL;DR: Decreased ezrin expression by shRNA reversed metastatic behaviors of human breast cancer cells by inducing c-src-mediated E-cadherin expression, suggesting that eZrin may have potential values in assessing lymphatic metastasis of human Breast cancer cells.

86 citations

Journal ArticleDOI
TL;DR: This study shows that the T7-system screening-based AdSiRNA can be used successfully to silence an oncogene and indicates that p28 GANK may serve as a novel therapeutic target for treating HCC.

86 citations

Journal ArticleDOI
TL;DR: Evidence is presented that the tumor cell‐derived platelet‐derived growth factor (PDGF) is the key regulator of vascular SMCs motility induced by breast cancer cells, and it is proposed that tumor‐derived PDGF and NRP‐1 of AOSMCs function as a relay system that promotes motility of vascularSMCs.
Abstract: Motility of vascular smooth muscle cells (SMCs) is an essential step for both normal and pathologic angiogenesis. We report here that breast tumor cells, such as MCF-7 and MDA-MB-231, can modulate this SMC migration. We present evidence that the tumor cell-derived platelet-derived growth factor (PDGF) is the key regulator of vascular SMCs motility induced by breast cancer cells. PDGF significantly upregulates neuropilin-1 (NRP-1) mRNA expression and protein production in aortic smooth muscle cells (AOSMCs) and depletion of NRP-1 production by AOSMCs with specific short hairpin RNA (shRNA) prevents the PDGF-dependent migration of vascular SMCs. Moreover, we demonstrate that PDGF physically interacts with NRP-1. We propose that tumor-derived PDGF and NRP-1 of AOSMCs function as a relay system that promotes motility of vascular SMCs.

86 citations

Patent
08 Jan 2009
TL;DR: In this article, a method for developing, generating and selecting tumor-free embryonic stem (ES)-like pluripotent cells using electroporation delivery of an inducible tumor suppressor mir-302 agent into mammalian cells is presented.
Abstract: The present invention generally relates to a method for developing, generating and selecting tumor-free embryonic stem (ES)-like pluripotent cells using electroporation delivery of an inducible tumor suppressor mir-302 agent into mammalian cells. More particularly, the present invention relates to a method and composition for generating a Tet-On/Off recombinant transgene capable of expressing a manually re-designed mir-302 microRNA (miRNA)/shRNA agent under the control of doxycyclin (Dox) in human somatic/cancer cells and thus inducing certain specific gene silencing effects on the differentiation-associated genes and oncogenes of the cells, resulting in reprogramming the cells into an ES-like pluripotent state.

86 citations


Network Information
Related Topics (5)
Cell culture
133.3K papers, 5.3M citations
91% related
Signal transduction
122.6K papers, 8.2M citations
90% related
Gene expression
113.3K papers, 5.5M citations
88% related
Regulation of gene expression
85.4K papers, 5.8M citations
86% related
Cellular differentiation
90.9K papers, 6M citations
86% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023804
2022477
2021384
2020454
2019541
2018518