Topic
Smoothelin
About: Smoothelin is a research topic. Over the lifetime, 264 publications have been published within this topic receiving 14069 citations.
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Journal Article•
TL;DR: MM and MP cells are of different antigenic characteristics that can be used in the microscopic diagnostics of selected cases and these results need to be validated in a series of specimens from transurethral resection.
Abstract: Background: Differentiation between the muscularis mucosae (MM) and muscularispropria (MP) of the bladder remains challenging. Objective: To identify MM- and MP-specific antigens that could be of potential value for staging of urothelialcarcinomain a pilot study. Method: The expression of 12 protein antigens in 11 human bladder specimens was examined. There were 5 post radical cystectomy specimens and 6 normal bladder autopsy specimens. Antibodies against actin, caldesmon, type IV collagen, cytokeratin, desmin, elastin, fibronectin, filamin, laminin, miotilin, smoothelin, and vimentin were used. Slides were stained with immunohistochemical reagents and assessed using light microscopy. The intensity of the immune reaction within MM and MP was evaluated in a four-level scale as negative, weakly, moderately, or strongly positive. Results: The presence of MM was noticed in 63.6% of the specimens.The expression of desmin, filamin, and smoothelin was stronger within MP compared to MM in all cases. Stronger reaction with anti-type IV collagen antibodies was noticed within MP in 80% of the cases. In the whole study group, the expression of vimentin was stronger within MM than MP. Conclusions: MM and MP cells are of different antigenic characteristics. This can be used in the microscopic diagnostics of selected cases. The results need to be validated in a series of specimens from transurethral resection.
4 citations
TL;DR: It was found that VSMC-like cells derived from CA expressed smoothelin gene and protein at lower levels and showed compromised contractility in response to vasoconstrictors, when compared with those derived from FA, and it was demonstrated that this negative effect of cryopreservation could be mediated by MEK1/2-ERK 1/2, IFNγ and Smad2/3 associated Signalling pathways.
4 citations
TL;DR: In this paper, the effects of nicotine on cervical TGF-β1/Smad3 pathway and fibroblast-myofibroblast differentiation regulated by this pathway in PTB-like models were investigated.
3 citations
TL;DR: In presence of an atypical imaging of the gallbladder, diagnosis of this group of congenital anomalies should be considered in order to adequately plan surgical intervention if necessary.
Abstract: Gallbladder duplication is a rare congenital anomaly, with an incidence of 1 in 3,800 autopsies. The correct diagnosis and treatment of this type of entity is important in clinical practice, because it may cause some clinical and surgical problems. In this report, we present the clinical case of a 28-year-old female with abdominal pain. Ultrasound of the upper abdomen showed a distended gallbladder with the presence of a septum that could suggest a congenital anomaly of the extrahepatic biliary system. During surgery, a distended and inflamed gallbladder with a lithiasis was found. In addition, a complete septum and double cystic duct were observed. The gross and histopathological evaluation of the surgical specimen allowed us to confirm the diagnosis of a Y- shaped type gallbladder duplication according to Boyden�s classification. In conclusion, in presence of an atypical imaging of the gallbladder, diagnosis of this group of congenital anomalies should be considered in order to adequately plan surgical intervention if necessary
3 citations
Journal Article•
TL;DR: DHT can induce bFGF expression and E2 promotes TGF beta2 expression, and the transformation toward smooth muscle cells induced by E(2) may involve the action of TGFbeta2.
Abstract: OBJECTIVE To study effects of androgen and estrogen on the expressions of basic fibroblast growth factor (bFGF) and transforming growth factor beta2 (TGFbeta2) in cultured human prostatic stromal cells. METHODS Human prostatic stromal cells obtained from 11 patients with benign prostatic hypertrophy (BPH) were cultured and stimulated with dihydrotestosterone (DHT) and estradiol (E2). The expressions of bFGF and TGFbeta2 mRNA along with smoothelin mRNA were observed with reverse transcriptase-polymerase chain reaction. RESULTS DHT significantly upregulated bFGF expression, and E2 enhanced TGFbeta2 and smoothelin expressions. A positive correlation between expressions of TGFbeta2 and smoothelin was observed. CONCLUSION DHT can induce bFGF expression and E2 promotes TGFbeta2 expression, and the transformation toward smooth muscle cells induced by E(2) may involve the action of TGFbeta2.
3 citations