Sodium ascorbate

About: Sodium ascorbate is a research topic. Over the lifetime, 869 publications have been published within this topic receiving 18589 citations. The topic is also known as: Sodium L-ascorbate & L-Ascorbic acid, monosodium salt.

Microbiological assay for serum, plasma, and red cell folate using cryopreserved, microtiter plate method.

Abstract: Publisher Summary This chapter presents microbiological assay for serum, plasma, and red cell folate, using cryopreserved, microtiter plate method. In the 500-ml beaker a solution of 0.5% (w/v) sodium ascorbate is prepared by dissolving 2.5 g of sodium ascorbate in 500 ml of water. This solution is used for preparing the working standard and for all assay dilutions. The working standard is prepared in the following way—a universal tube containing stock standard solution is brought to room temperature and a 50- μ l aliquot is taken and diluted to 100 ml with 0.5% (w/v) sodium ascorbate in a volumetric flask. This solution is mixed thoroughly, then 5 ml is taken and diluted to 100 ml with 0.5% (w/v) sodium ascorbate in a second volumetric flask to give a final working standard concentration of 500 ng/liter. Duplicate 50- μ l aliquots of serum or plasma or duplicate 25- μ l aliquots of whole-blood lysate are pipetted into labeled 4-ml polypropylene tubes. Using the adjustable repetitive sampling pipette the aliquots are diluted to a total of 1 ml with 0.5% (w/v) sodium ascorbate.

Reversal of Compromised Bonding to Oxidized Etched Dentin

Abstract: The mechanism responsible for hydrogen-peroxide- or sodium-hypochlorite-induced reductions in dentin bond strength is unknown. This in vitro study tested the hypothesis that these oxidizing agents were responsible by attempting to reverse the effect with sodium ascorbate, a reducing agent. Human dentin was treated with these oxidants before or after being acid-etched and with or without post-treatment with sodium ascorbate. They were bonded with either Single Bond or Excite. Hydrogen peroxide reduced the bond strengths of both adhesives, while sodium hypochlorite produced reduction in adhesion of only Single Bond (p < 0.05). Following treatment with sodium ascorbate, reductions in bond strength were reversed. Transmission and scanning electron microscopy showed partial removal of the demineralized collagen matrix only by sodium hypochlorite. The observed compromised bond strengths cannot be attributed to incomplete deproteinization and may be related to changes in the redox potential of the bonding substrates.