Solid lipid nanoparticle

About: Solid lipid nanoparticle is a research topic. Over the lifetime, 3175 publications have been published within this topic receiving 127912 citations. The topic is also known as: LNP & SLN.

Topical administration of cyclosporin A in a solid lipid nanoparticle formulation.

Abstract: Cyclosporin A (CsA)-loaded solid lipid nanoparticles (SLN) were developed for improved skin penetration. CsA-loaded SLN, prepared using a hot homogenization method, were nano-sized (about 73 nm) with a slightly negative surface charge (about -16 mV) and stable under physiological conditions regardless of CsA incorporation. In vitro permeation studies using murine skin mounted in the Franz-type vertical diffusion assembly revealed that the skin permeation efficiency of CsA-loaded SLN was 2-fold higher than that of CsA-oil mixture in viable skin. Furthermore, topically administered CsA-loaded SLN relieved symptoms of atopic dermatitis (AD) in an in vivo murine model of AD by decreasing the T helper (Th) 2 cell-related cytokines interleukin (IL)-4 and -5. These results suggest that SLN are effective drug carriers for topical delivery andthat CsA-loaded SLN can be therapeutically applied in allergy-related skin disorders.

Characterization of Nigella Sativa L. Essential Oil-Loaded Solid Lipid Nanoparticles

Abstract: Problem statement: Seeds of Nigella sativa L., commonly known as black seed, have been used in traditional medicine by many Asian, Middle Eastern and Far Eastern Countries to treat headache, coughs, abdominal pain, diarrhea, asthma, rheumatism and other diseases. The seeds of this plant are the most extensively studied, both phytoc hemically and pharmacologically. The aqueous and oil extracts of the seeds have been shown to posses s especially nowadays in pharmaceutical antioxidant, anti-inflammatory, anticancer, analges ic, antimicrobial activities and medicinal and cosmetic applications, sanitary, cosmetic, agricult ural and food industries. Approach: The aim of this study was to formulate a new delivery system for de rmal and cosmetic application by the incorporation of Nigella sativa essential oil into solid lipid nanoparticles SLN. S LN formulations were prepared following the high-pressure homogenization after st arring and ultra-trax homognization techniques using hydrogenated palm oil Softisan 154 and N. sativa essential oil as lipid matrix, sorbitol and water as surfactants. The SLN formulation particle size w as determined using Photon Correlation System (PCS). Results: The change of particle charge was studied by Zeta Potential (ZP) measurements, while the melting and re-crystallization behavior was stu died using Differential Scanning Calorimetry (DSC). Data showed a high physical stability for bo th formulations at various storage temperatures during 3 months of storage. In particu lar, average diameter of N. sativa essential oil- loaded SLN did not vary during storage and increase d slightly after freeze-drying the SLN dispersions. Conclusion: Therefore, obtained results showed that the studie d SLN formulations are suitable carriers in pharmaceutical and cosmetic fi elds.

Effect of physical state (solid vs. liquid) of lipid core on the rate of transport of oxygen and free radicals in solid lipid nanoparticles and emulsion

Abstract: Solid lipid nanoparticles (SLNs) may have significant potential to limit oxidation of encapsulated bioactive compounds. This is based on the hypothesis that the solid core in SLNs can significantly limit the rate of transport of oxygen and free radicals into the lipid core from the aqueous phase. In this study, we have directly compared the rate of transport of oxygen and free radicals in SLNs and liquid emulsion prepared from the same lipid material (eicosane) at a fixed temperature. Eicosane nanoparticles stabilized by high melting lecithin and bile salts were used as a model system. The physical state (solid vs. liquid) of the lipid phase was engineered using the supercooling phenomenon of emulsified eicosane. Transport of oxygen and free radicals was measured based on changes in fluorescence intensity of oxygen or peroxyl radical sensitive dyes encapsulated in the lipid phase upon exposure to either air or peroxyl radicals generated in the aqueous phase. The results showed that the rate of oxygen transport was marginally reduced in SLNs as compared to liquid emulsion, while the rate of transport of peroxyl free radicals was not significantly affected by the physical state of the lipid core in SLNs and emulsion. Together, these results indicated that the solid core of SLNs does not significantly reduce the rate of transport of oxygen or free radicals as compared to the liquid core emulsion. To address this paradox, the distribution of encapsulated dye was characterized in both SLNs and emulsion using fluorescence imaging. The results showed significant redistribution of the encapsulated dye molecules with formation of the solid lipid core. In contrast to homogeneous distribution in the liquid emulsion, SLNs showed higher concentration of the dye at the periphery as compared to the center of the lipid droplet. The expulsion of encapsulated molecules to the surface of SLNs can potentially limit the ability of the solid core to protect encapsulated products from oxygen and free radicals.