Showing papers on "Solid-state fermentation published in 1986"

Solid state fermentation of apple pomace for citric acid production

Abstract: A solid state fermentation process is described for the production of citric acid from apple pomace byAspergillus niger NRRL 567. The yields of citric acid varied with the pomace varieties, and were dependent on (1) the amount of methanol present in the pomace, and (2) the fermentation time and temperature. The process yielded as much as 90 g citric acid/kg apple pomace fermented in the presence of 3–4% methanol at 30°C in five days.

Solid-state fermentation of grape pomace for ethanol production

Abstract: Grape pomace was used as a substrate for the production of ethanol under solid-state fermentation conditions. The yield of ethanol amounted to greater than 80% of the theoretical, based on the fermentable sugar consumed.

Utilization of Grape Pomace for Citric Acid Production by Solid State Fermentation

Abstract: A solid state fermentation method has been developed and is described for citric acid production from grape pomace by Aspergillus niger NRRL 567. The yields of citric acid varied with the pomace varieties and were dependent on the amount of methanol present in the pomace and the fermentation time and temperature. Under optimum fermentation conditions, this method reduced the pomace dry matter by about 43% and yielded more than 90 g of citric acid per kg of grape pomace fermented. The yield was more than 60% based on the amount of fermentable sugar consumed. These results suggest that the use of grape pomace for fungal production of citric acid could represent an efficient method of minimizing the pomace disposal problems and concomitantly producing a commercially valuable organic acid.

A model substrate for solid-state fermentation

Abstract: A model substrate consisting of cassava starch embedded in kappa-carrageenan was used to mimic the growth ofRhizopus oligosporus on cassava tubers. Growth on the model substrate was similar to that during solid-state fermentation of the actual cassava. However, protein production and starch utilization were slower on the model substrate.

Production of xylanases by Chaetomium cellulolyticum during growth on lignocelluloses.

Abstract: Chaetomium cellulolyticum was able to produce xylanases on all the three lignocelluloses: wheat straw, corn stover and aspen wood. Wheat straw was the best. Solid state fermentation of lignocelluloses gave higher yields of xylanases than liquid state fermentation, but it took longer time of incubation.

Protease production with sweet potato residue by solid state fermentation.

Abstract: The sweet potato residue being at the initial moisture content 50-58%, initial pH 3.5-4.3, supplemented with rice bran, and minerals, and incubated at 20-26 degrees C for 5 days was the optimal conditions for protease production with Aspergillus niger NTU-AM-1 by solid state fermentation. Protease could be recovered by shaking at room temperature for one hour and extracted with five times volume of 0.1% NaCl solution. The yield of protease was 814 units per gram dry weight of substrate. Partially purified protease with DEAE-Sephacel column chromatography was thermally stable and able to retain 80-100% of activity in pH 4.0-5.5 at 55 degrees C for 40 minutes. In addition, the activity of protease was stimulated by the presence of EDTA and cysteine, but was inhibited by the addition of HgCl2.

Effects of heat and microbiological pretreatments of wheat straw substrate on the initial phase of solid state fermentation (ssf) by white rot fungi

Abstract: Quantitative differentiation of microbial activity and saprophytic colonisation in wheat straw substrate during SSF is described Wheat straw contains an active indigenous microbial flora In both untreated and heat pretreated substrates a peak of microbial respiratory activities occurs generally after 24 h of fermentation; addition of actidione and streptomycin has no marked inhibitory effect Quantitative differences between endogenous microbial respiration and respiration byPleurotus during the initial four days of SSF are least in the heat pretreated substrates and greater when microbiological pretreatment is used In treatments subjected to 48 h anaerobic fermentation at 50°C, saprophytic colonization similar to that observed in sterile substrates is obtained

Optimization of solid-state fermentation of citrus dried peel by aspergillus niger in a packed bed column

Abstract: The protein enrichment of citrus dried peel on solid-state fermentation by Aspergillus niger in packed bed column was optimized using a two factor central composit design with α = 2 considering the “initial moisture content of the fermentation mass” and the “temperature in the centre of the fermenter”. It results significance for the moisture content (optimum 56%) and non significance for the temperature in the conditions employed. This indicates that a considerable range of temperature increase for the investigated SSF system could be allowed.

Spore production ofPenicillium roqueforti by simulated solid state fermentation

Abstract: A culture technique, based on the growth of a microorganism on inert porous particles (e. g. pozzolano) impregnated and continuously fed with substrate is applied to the growth and spore production ofPenicillium roqueforti. The composition and the feed rate of the medium can be controlled, and the biomass is directly estimated.P. roqueforti exhibits a diauxic growth on the medium containing sucrose and malt extract used, and 1.5 109 spores/g pozzolano may be obtained.

Protein enrichment of sugar beet residue with the inoculation of conidia of Trichoderma album by solid state fermentation.

Abstract: Cellulosic material was inoculated with Trichoderma album by solid state fermentation and the change of protein, total nitrogen, mineral nitrogen, cellulose and total fiber contents of raw material were measured. It was found that the water holding capacity and bulk density of substrate increased as the fermentation progressed. The optimum C/N ratio for the conidia production was between 4 and 7, and the optimum pH was around 5. The protein enrichment of sugar beet residue were the best at the initial moisture content 76%, initial pH 4.5 and supplement with 1% nitrogen. After 4 days fermentation, the final product contained 22% of protein. From the present state of technological development, the protein enrichment of cellulosic materials by solid state fermentation proved to be valuable.