Showing papers on "Solid-state fermentation published in 1989"
TL;DR: The antibiotic production was consistent with the morphogenesis of S. viridifaciens: activity could be detected on the third day, had the maximal potency on the sixth day, and decreased slightly on the tenth day.
Abstract: For saving energy in antibiotic production and reducing the amount of agricultural wastes, solid state fermentation was used in this study to produce tetracycline with sweet potato residue by Streptomyces viridifaciens ATCC 11989. It was found that the optimal media for tetracycline production were sweet potato residue 100 g, organic nitrogen (rice bran, wheat bran, or peanut meal) 20 g, (NH(4))(2)SO(4) 2.4 g, KH(2)PO(4) 0.4 g, CaCO(3) 1.8 g, NaCl 0.6 g, MgCl(2) 0.8 g, soluble starch 10 g, methionine 0.2 g, histidine 0.8 g, and monosodium glutamate 1.6 g with initial moisture content 68-72%, and initial pH 5.8-6.0. Each gram of dry weight substrate was inoculated with 1.0 x 10(8) conidia and incubated at 26 degrees C for 5-7 days, producing 4720 mug of total tetracycline equivalent potency. When incubated at 26 degrees C with the initial moisture content 68%, the conidia in solid media germinated on the second day, mycelia grew abundantly on the third day and reached stationary phase on the sixth day. The antibiotic production was consistent with the morphogenesis of S. viridifaciens: activity could be detected on the third day, had the maximal potency on the sixth day, and decreased slightly on the tenth day. (11-3-88 tly).
95 citations
TL;DR: Bacillus licheniformis M27 produced 21,000 units of alpha-amylase/g dry bacterial bran under solid state fermentation in wheat bran medium enriched with 3.3% di-ammonium hydrogen phosphate, showing pH optima at 6.5–7.0 and activity over the pH range 6.0–10.5.
Abstract: Bacillus licheniformis M27 produced 21, 000 units of alpha-amylase/g dry bacterial bran under solid state fermentation in wheat bran medium enriched with 3.3% di-ammonium hydrogen phosphate. The crude enzyme, with temperature optimum at 90°C in 0.5% starch solution, showed pH optima at 6.5–7.0 and 9.5 and over 75% activity over the pH range 6.0–10.5.
50 citations
TL;DR: Fermentations at the 50 g scale showed that the white-rot fungus Phlebia tremellosa can delignify aspen wood and increase the accessibility of its polysaccharides to enzymatic hydrolysis, under solid-state fermentation conditions.
49 citations
TL;DR: In this paper, the effects of selected culture parameters on the solid-state fermentation of radiata pine (Pinus radiata) by the brown-rot fungus Gloeophyllum trabeum were examined.
41 citations
TL;DR: In order to select fungi with capacity to produce pectic enzymes when citrus pulp pellets were used in solid cultures, a collection of one hundred fungi was screened.
Abstract: In order to select fungi with capacity to produce pectic enzymes when citrus pulp pellets were used in solid cultures we screened a collection of one hundred fungi
32 citations
TL;DR: In this article, the production of cellulases and β-glucosidase using locally-isolated Aspergillus niger on various cheap sources of cellulose like bagasse, corn corbs, computer cards and sawdust was studied.
30 citations
TL;DR: In this article, the process of solid substrate fermentation (SSF) using Coprinus fimetarius 386 on rice straw on a pilot scale was standardized and evaluated by conducting feeding trials on goats, in comparison with urea (ammonia)-treated straw.
27 citations
TL;DR: Solid state fermentation systems were developed for the production of cephalosporins with Streptomyces clavuligerus and CephalOSporium acremonium and S. clavULigerus NRRL 3585 was grown on moistened barley.
Abstract: Solid state fermentation systems were developed for the production of cephalosporins withStreptomyces clavuligerus andCephalosporium acremonium. S. clavuligerus NRRL 3585 was grown on moistened barley under optimum solid state fermentation conditions for 7 days; approximately 300 μg cephalosporins per g substrate were extracted from the kernels.C. acremonium C-10 produced approximately 950 μg cephalosporin C per g substrate after 10 days of solid state fermentation.
26 citations
TL;DR: Membrane filters overlaid on slabs of a model solid substrate enabled recovery of biomass of Rhizopus oligosporus and provides a useful tool for studying solid-state fermentation.
Abstract: Membrane filters overlaid on slabs of a model solid substrate enabled recovery of biomass ofRhizopus oligosporus. Although the presence of the membrane filter affects the growth ofRhizopus oligosporus it provides a useful tool for studying solid-state fermentation.
25 citations
Journal Article•
TL;DR: In this article, the results obtained from two strategies of spore production of Penicillium roquefortii by solid state fermentation techniques are presented, one involves growing the fungus on support particles which may consist of a natural starchy substrate e.g., buckwheat grains, or inert porous particles such as pozzolano impregnated with a liquid nutrient.
18 citations
Dissertation•
01 Jan 1989
TL;DR: Considering the quantitative importance of cereal straws and the need for feeding these residues to the large populations of livestocks especially in developing countries, considerable efforts have been made to upgrade their nutritive value as discussed by the authors.
Abstract: Considering the quantitative importance of cereal straws and the need for feeding these residues to the* large populations of livestocks especially in developing countries, considerable efforts have been made to upgrade their nutritive value. Recent biotechnological approaches utilising efficient and improved micro-organisms for bioconversion of cereal crop residues with supplementation under optimised fermentation conditions has provided enough basic knowledge and encouragement to produce' a wholesome feed with higher digestible organic matter and crude protein
Journal Article•
TL;DR: It was found that for a maximal protein yield the medium ought to contain about: 60% of water, 36% of starchy raw materials d.m. (e.g. 28.8% of coarse rye meal and 7.2% of beet pulp), and 0.85% of nitrogen sources (1.6% of (NH4)2SO4 and 1.1% of urea).
Abstract: Study was made of the effect of medium humidity, source and dose of nitrogen, dose of phosphorus, dose of inoculum and aeration on protein biosynthesis by strain Aspergillus oryzae A. or. 11 in solid state fermentation. It was found that for a maximal protein yield the medium ought to contain about: 60% of water, 36% of starchy raw materials d. m. (e.g. 28.8% of coarse rye meal and 7.2% of beet pulp), 0.85% of nitrogen sources (1.6% of (NH4)2SO4 and 1.1% of urea), and 0.35% of phosphorus source (1.3% of KH2PO4). pH of medium should be near 6.5. The dose of inoculum should not be lower than 108 spores/100 g medium.
The duration of culture ought to be 20–22 h at 30–35°C, with aeration at least 40 dm3 of air/h × 1000 g medium. Under these conditions the protein yield is about 6.0–6.3 g/100 g of starting medium d.m. at the cost of utilization of about 25 g total carbohydrates.
Journal Article•
TL;DR: The problem of marketing a highly viscous enzyme preparation was solved by reducing the viscosity by 78% by conditioning the crude extract with 1% (w/v)CaCl 2 •2H 2 O at 29 o C for 60 minutes.
TL;DR: In this paper, the effect of the culture method in solid medium and the influence of starch-containing raw materials on the yield of fungal protein biosynthesis were studied, and three procedures (laboratory bioreactor method, tray method, and pile method) gave satisfactory results.
Abstract: The effect of the culture method in solid medium and the influence of starch-containing raw materials on the yield of fungal protein biosynthesis were studied. Three procedures (laboratory bioreactor method, tray method and pile method) gave satisfactory results. Protein yields amounted to 5.0–5.9 g/100 g of starting medium d.m. upon utilization of 20.9–24.1 g carbohydrates. The procedure involving the use of a fermenter with a mixer afforded protein yields by about 50% lower as compared with the three above-mentioned procedures, and therefore it requires technical improvement.
As a result of fungal culture using various starch-containing raw materials, the protein content in post-culture products increased by 46–88%, as compared with starting medium. The contents of 13 amino acids (including some exogenous ones which increased by 52–82%) in post-culture products substantially rose (by 34–63%). The post-culture products exhibited proteolytic activity (1260–3500 HU/g of d.m.).
The kind of the source of starch evidently influenced protein biosynthesis. Media based on potatoes afforded the greatest increases in protein (5.8–5.9 g/100 g of starting medium d.m.), and those containing coarse rye meal and milling by-products — the smallest ones (4.5–4.6 g/100 g of starting medium d.m.).
TL;DR: In this article, a conventional mixer was modified for use as a gelatinization reactor (10 − 15 kg of dry material), which had a built-in horizontal stirrer and a jacket heated with saturated steam.