Solid-state fermentation

About: Solid-state fermentation is a research topic. Over the lifetime, 5311 publications have been published within this topic receiving 113337 citations.

Solid-State Fermentation Technology for Bioconversion of Biomass and Agricultural Residues

Abstract: Solid-state fermentations (SSF) have attracted a renewed interest and attention from researchers due to recent developments in the field of microbial-biotechnology. Hence, for the practical, economical and environmentally-friendly bioconversion of agro-industrial wastes, solid state or substrate fermentation has been researched globally and proved to be the ideal technology for this purpose. In this chapter some important aspects of solid-state cultivation system have been discussed, including the variety of substrates and microorganisms used in SSF for the production of various end products; and the performance control of system by regulation of important factors.

Utilisation of By-Products from Sunflower-Based Biodiesel Production Processes for the Production of Fermentation Feedstock

Abstract: By-products streams from a sunflower-based biodiesel plant were utilised for the production of fermentation media that can be used for the production of polyhydroxyalkanoates (PHA). Sunflower meal was utilised as substrate for the production of crude enzyme consortia through solid state fermentation (SSF) with the fungal strain Aspergillus oryzae. Fermented solids were subsequently mixed with unprocessed sunflower meal aiming at the production of a nutrient-rich fermentation feedstock. The highest free amino nitrogen (FAN) and inorganic phosphorus concentrations achieved were 1.5 g L−1 and 246 mg L−1, respectively, when an initial proteolytic activity of 6.4 U mL−1 was used. The FAN concentration was increased to 2.3 g L−1 when the initial proteolytic activity was increased to 16 U mL−1. Sunflower meal hydrolysates were mixed with crude glycerol to provide fermentation media that were evaluated for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) using Cupriavidus necator DSM 545. The P(3HB-co-3HV) (9.9 g l−1) produced contained 3HB and 3HV units with 97 and 3 mol %, respectively. Integrating PHA production in existing 1st generation biodiesel production plants through valorisation of by-product streams could improve their sustainability.

Laccase production by Coriolopsis caperata RCK2011: Optimization under solid state fermentation by Taguchi DOE methodology

Abstract: Laccase production by Coriolopsis caperata RCK2011 under solid state fermentation was optimized following Taguchi design of experiment. An orthogonal array layout of L18 (21 × 37) was constructed using Qualitek-4 software with eight most influensive factors on laccase production. At individual level pH contributed higher influence, whereas, corn steep liquor (CSL) accounted for more than 50% of the severity index with biotin and KH2PO4 at the interactive level. The optimum conditions derived were; temperature 30°C, pH 5.0, wheat bran 5.0 g, inoculum size 0.5 ml (fungal cell mass = 0.015 g dry wt.), biotin 0.5% w/v, KH2PO4 0.013% w/v, CSL 0.1% v/v and 0.5 mM xylidine as an inducer. The validation experiments using optimized conditions confirmed an improvement in enzyme production by 58.01%. The laccase production to the level of 1623.55 Ugds−1 indicates that the fungus C. caperata RCK2011 has the commercial potential for laccase.