Topic
Solid-state fermentation
About: Solid-state fermentation is a research topic. Over the lifetime, 5311 publications have been published within this topic receiving 113337 citations.
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TL;DR: The phytase production by Sporotrichum thermophile TLR50 was recorded on all the commonly used animal feed ingredients tested to varying degrees in solid-state fermentation and an overall 76% enhancement was achieved owing to optimization.
Abstract: The phytase production by Sporotrichum thermophile TLR50 was recorded on all the commonly used animal feed ingredients tested to varying degrees in solid-state fermentation. Enzyme production increased to 180 U/g of dry moldy residue (DMR) in sesame oil cake at 120 h and 45°C at the initial substrate-to-moisture ratio of 1∶2.5 and a
w
of 0.95. Supplemenetation of sesame oil cake with glucose and ammonium sulfate further enhanced phytase titer (282 U/g of DMR). An overall 76% enhancement in phytase production was achieved owing to optimization. The mold secreted acid phosphatase, amylase, xylanase, and lipase along with phytase. By the action of phytase, inorganic phosphate was liberated efficiently, leading to dephytinization of sesame oil cake.
61 citations
TL;DR: Solid-state fermentations were carried out for high yield of poly-gamma-glutamic acid (Gamma-PGA) by Bacillus subtilis CCTCC202048 to lay a foundation for lessening the pollution of swine manure, increasing fertilizer efficiency and exploring a late-model organic fertilizer that retains water and nutrients.
61 citations
TL;DR: The comparative production of GA3 by solid-state fermentation and submerged fermentation indicated better productivity with the former technique, mainly with pretreated substrate, and higher yields ofGA3 were achieved using a mixed substrate comprising coffee husk and cassava bagasse, increasing the results twice.
Abstract: Five strains of Gibberella fujikuroi and one of Fusarium moniliforme were screened for the production of gibberellic acid (GA3) in coffee husk, and based on the results, one strain, G. fujikuroi LPB-06, was selected. The comparative production of GA3 by solid-state fermentation and submerged fermentation indicated better productivity with the former technique, mainly with pretreated substrate. The GA3 accumulation was 6.1 times higher in the case of solid-state fermentation. Considering the C:N relation, higher yields of GA3 were achieved using a mixed substrate comprising coffee husk and cassava bagasse (7:3, dry wt), increasing the results twice. Supplementation of an optimized saline solution containing 0.03% FeSO4 and 0.01% (NH4)2SO4 enhanced the accumulation of GA3 1.7 times in the fermented substrate. Under the finally optimized condition, the culture gave a maximum of 492.5 mg of GA3/kg of dry substrate, with a pH of 5.3, moisture of 75%, and incubation temperature of 29 degrees C. GA3 yield was almost 13 times more than the initial results.
60 citations
TL;DR: This work investigated the enzymes that enable Grifola frondosa, to colonize and deconstruct two formulations based on industrial lignocellulosic by-products, and found that enzyme activities peaked during colonization but declined drastically during fruiting body formation.
Abstract: Cultivation of specialty mushrooms on lignocellulosic wastes represents one of the most economical organic recycling processes. Compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by the edible and medicinal fungus Grifola frondosa, the parameters affecting their production, and enzyme activity profiles during different stages of the developmental cycle. In this work we investigated the enzymes that enable G. frondosa, to colonize and deconstruct two formulations based on industrial lignocellulosic by-products. G. frondosa degraded both substrates (oak-sawdust plus corn bran, and oak/corn bran supplemented with coffee spent-ground) decreasing 67 and 50% of their lignin content, along with 44 and 37% of the polysaccharides (hemicellulose and cellulose) respectively. 35.3% biological efficiency was obtained when using oak sawdust plus corn bran as substrate. Coffee spent-ground addition inhibited mushroom production, decreased growth, xylanase and cellulase activities. However, taking into account that G. frondosa successfully colonized this residue; this substrate formula might be considered for its growth and medicinal polysaccharide production. Although G. frondosa tested positive for Azure B plate degradation, a qualitative assay for lignin-peroxidase, attempts to detect this activity during solid state fermentation were unsuccessful. Enzyme activities peaked during colonization but declined drastically during fruiting body formation. Highest activities achieved were: endoglucanase 12.3, exoglucanase 16.2, β-glucosidase 2.3, endoxylanase 20.3, amylase 0.26, laccase 14.8 and Mn-peroxidase 7.4 U/g dry substrate.
60 citations
TL;DR: Dried, ground and resuspended fermented rice was the most pratical and effective inoculum preparation, although, in the laboratory, spore suspensions prepared directly from agar slants were more convenient.
Abstract: Rice bran was superior to other proteinaceous substrates for protease production by Rhizopus oligosporus ACM 145F in solid-state fermentation. Maximum protease yield was after 72 h. The optimal initial moisture content was 47% (a w=0.97). Dried, ground and resuspended fermented rice was the most pratical and effective inoculum preparation, although, in the laboratory, spore suspensions prepared directly from agar slants were more convenient. Inoculum density (from 10(2) to 10(7) spores/g substrate) and age (3, 5, 7 and 9 days) had little effect on protease yield.
60 citations