Topic
Solid-state fermentation
About: Solid-state fermentation is a research topic. Over the lifetime, 5311 publications have been published within this topic receiving 113337 citations.
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TL;DR: The results indicate the potential of P. lilacinum CFRNT12 for the chitosanase production employing cost effective SSF using shrimp by-products as substrate and indicates the aptness of the model.
44 citations
TL;DR: Fermentations were consistent in terms of cellulase production and microbial communities, and the higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulases production at pilot scale.
44 citations
TL;DR: This study provided a novel method to produce γ-PGA from abundant and low-cost agroindustrial residues through solid-state fermentation with dry mushroom residues and monosodium glutamate production residues for the first time.
44 citations
TL;DR: Several fold increase in endo-chitinase and β-N-acetylhexosaminidase activities in the crude enzymes preparation was achieved after concentrating with polyethylene glycol, indicating the potential of these enzymes for efficient production of GlcNAc from α- chitin.
Abstract: Soil isolates of mesophilic Penicillium monoverticillium CFR 2, Aspergillus flavus CFR 10 and Fusarium oxysporum CFR 8 were cultivated in solid state fermentation (SSF) using wheat bran solid medium supplemented with α-chitin in order to produce chitinolytic enzyme. Under SSF cultivation, maximum enzymes (U/g IDS) production was 41.0 (endo-chitinase) and 195.4 (β-N-acetylhexosaminidase) by P. monoverticillium, 26.8 (endo-chitinase) and 222.1 (β-N-acetylhexosaminidase) by A. flavus and 13.3 (endo-chitinase) and 168.3 (β-N-acetylhexosaminidase) by F. oxysporum after 166 h of incubation. The crude endo-chitinase and β-N-acetylhexosaminidase derived from A. flavus and F. oxysporum revealed optimum temperature at 62 ± 1°C, but the enzymes from P. monoverticillium showed optimum temperature at 52 ± 1°C for maximum activity. Several fold increase in endo-chitinase and β-N-acetylhexosaminidase activities in the crude enzymes preparation was achieved after concentrating with polyethylene glycol. The concentrated crude chitinases from P. monoverticillium, A. flavus and F. oxysporum, respectively yielded 95.6, 96.6 and 96.1 mmol/l of N-acetyl-d-glucosamine (GlcNAc) in 48 h of reaction from colloidal chitin. While, the crude enzyme preparations of P. monoverticillium, A. flavus and F. oxysporum produced 10.11, 6.85 and 10.7 mmol/l of GlcNAc respectively, in 48 h of reaction from crystalline α-chitin. HPLC analysis of colloidal chitin hydrolysates prepared with crude chitinases derived from P. monoverticillium, A. flavus and F. oxysporum revealed that the major reaction product was monomeric GlcNAc (~80%) and a small amount of (GlcNAc)4 (~20%), indicating the potential of these enzymes for efficient production of GlcNAc from α-chitin.
44 citations
44 citations