Solid-state fermentation

About: Solid-state fermentation is a research topic. Over the lifetime, 5311 publications have been published within this topic receiving 113337 citations.

Kinetics of Gibberella fujikuroi growth and gibberellic acid production by solid-state fermentation in a packed-bed column bioreactor.

Abstract: In this work the growth of Gibberella fujikuroi and gibberellic acid (GA3) production were studied using coffee husk and cassava bagasse as substrates in a packed-bed column bioreactor connected to a gas chromatograph for exit gas analysis With the respirometric data, a logarithmic correlation between accumulated CO2 and biomass production was determined, and the kinetics of the fungal growth was compared for estimated and experimental data The solid medium consisted of coffee husk (pretreated with alkali solution), mixed with cassava bagasse (7:3 dry weight basis), with a substrate initial pH of 52 and moisture of 77% Cultivation was carried out in glass columns, which were packed with preinoculated substrate and with forced aeration of 024 L of air/[h (g of substrate)] for the first 3 days, and 072 L of air/[h (g of substrate)] for the remaining period The maximum specific growth rate (microm) obtained was 0052 h(-1) (between 24 and 48 h of fermentation) A production of 0925 g of GA3/kg of substrate was achieved after 6 days of fermentation

PRODUCTION OF LIGNINOLYTIC ENZYMES BY SOLID-STATE FERMENTATION USING Pleurotus eryngii

Abstract: Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ability to produce various ligninolytic enzymes such as laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) by solid-state fermentation (SSF), which was carried out using a support substrate from the fruit juice industry. The chemical content of grape waste from this industry was studied. Also, the production patterns of these extracellular enzymes were researched during the growth of the organism for a period of 20 days and the protein, reducing sugar, and nitrogen levels were monitored during the stationary cultivation. The highest Lac activity was obtained as 2247.62 ± 75 U/L on day 10 in the presence of 750 µM Mn2+, while the highest MnP activity was attained as 2198.44 ± 65 U/L on day 15 in the presence of 500 µM Mn2+. Decolorization of methyl orange and reactive red 2 azo dyes was also achieved with ligninolytic enzymes, produced in SSF of P. eryngii.

Production of cellulase from Aspergillus terreus MS105 on crude and commercially purified substrates.

Abstract: Aspergillus terreus MS105 was originally isolated from soil and screened for cellulase production in the presence of various carbon sources including carboxymethyl cellulose (CMC), avicel, sigmacell, filter-paper and salicin. CMC induced the production of endoglucanase (EG) and filter-paperase while the levels of β-glucosidase (BGL) were increased when salicin was present in the medium. Nature of production medium influenced the duration of lag- and log-phase of the growth, rate of fungal dry-mass and enzyme production. The volumetric and specific productivity of cellulase under submerged fermentation of grass were 1.7–20-folds higher than sugarcane-bagasse, corncob and commercially available purified substrates. Nonetheless, solid state fermentation (SSF) of crude substrates also yielded high volumetric productivity of EG and BGL. The studies on characterization of enzymes showed that EG was more thermostable than BGL with an optimum activity at 70 °C and a melting temperature of 76 °C. A 1.2–1.5-folds increase in EG activity was observed in the presence of K+, Ca2+ and Mg2+, whereas, the EG and BGL activities remained unaffected in the presence of EDTA. Both the enzyme activities performed optimally under acidic range of pH.