Showing papers on "Speckle imaging published in 2013"

Low-cost laser speckle contrast imaging of blood flow using a webcam.

Abstract: Laser speckle contrast imaging has become a widely used tool for dynamic imaging of blood flow, both in animal models and in the clinic. Typically, laser speckle contrast imaging is performed using scientific-grade instrumentation. However, due to recent advances in camera technology, these expensive components may not be necessary to produce accurate images. In this paper, we demonstrate that a consumer-grade webcam can be used to visualize changes in flow, both in a microfluidic flow phantom and in vivo in a mouse model. A two-camera setup was used to simultaneously image with a high performance monochrome CCD camera and the webcam for direct comparison. The webcam was also tested with inexpensive aspheric lenses and a laser pointer for a complete low-cost, compact setup ($90, 5.6 cm length, 25 g). The CCD and webcam showed excellent agreement with the two-camera setup, and the inexpensive setup was used to image dynamic blood flow changes before and after a targeted cerebral occlusion.

Deep tissue flowmetry based on diffuse speckle contrast analysis.

Abstract: Diffuse correlation spectroscopy (DCS) is an emerging modality for noninvasive deep tissue blood flow monitoring that is becoming increasingly popular; it conducts an autocorrelation analysis of fast fluctuating photon count signals from a single speckle. In this Letter, we show that the same level of deep tissue flow information can be obtained from a much simpler analysis on the spatial distribution of the speckles that is obtained by a CCD camera, which we named diffuse speckle contrast analysis (DSCA). Both the flow phantom experiment and in vivo cuff occlusion data are presented. DSCA can be considered a new optical modality that combines DCS and laser speckle contrast imaging (LSCI), which exploits simple instrumentation and analysis and yet is sensitive to deep tissue flow.

3D dynamic holographic display by modulating complex amplitude experimentally.

Abstract: Complex amplitude modulation method is presented theoretically and performed experimentally for three-dimensional (3D) dynamic holographic display with reduced speckle using a single phase-only spatial light modulator. The determination of essential factors is discussed based on the basic principle and theory. The numerical simulations and optical experiments are performed, where the static and animated objects without refinement on the surfaces and without random initial phases are reconstructed successfully. The results indicate that this method can reduce the speckle in reconstructed images effectively; furthermore, it will not cause the internal structure in the reconstructed pixels. Since the complex amplitude modulation is based on the principle of phase-only hologram, it does not need the stringent alignment of pixels. This method can be used for high resolution imaging or measurement in various optical areas.

Sub-Rayleigh imaging via speckle illumination.

Abstract: We demonstrate sub-Rayleigh limit imaging of an object via speckle illumination. Imaging beyond the conventional Rayleigh limit is achieved by illuminating the object with pseudothermal light that exhibits a random speckle pattern. An object image is reconstructed from the second-order correlation measurement and the resolution of the image, which exceeds the Rayleigh limit, is shown to be related to the size of the speckle pattern that is tied to the lateral coherence length of the pseudothermal light.

Speckle noise suppression in digital holography by angular diversity with phase-only spatial light modulator

Abstract: A speckle noise suppression method in digital holography is proposed by the angular diversity with a phase-only spatial light modulator (SLM). The minimal angular difference of illumination beams is quantitatively analyzed to ensure the noncorrelation of any two speckle patterns, and then the phase-only SLM is employed to generate a series of tilted illumination beams. Comparing with the typical methods, the tilted illumination beams are controlled dynamically and accurately, which makes it possible to record a large number of holograms. Finally, using an image-plane digital holographic system, 117 holograms are recorded respectively, and the synthesized reconstructed images are obtained with the greatly suppressed speckle noise which is in good agreement with the theoretical results. The experimental results demonstrate the effectiveness, repeatability, and practicability of the proposed approach.

Averaging techniques for OCT imaging

Abstract: State-of-the-art Fourier-domain optical coherence tomography (OCT) allows for the acquisition of up to millions of spectral fringes per second. This large amount of data can be used to improve the quality of structural tomograms after effective averaging. Here, we compare three OCT image improvement techniques: magnitude averaging, complex averaging, and spectral and time domain OCT (STdOCT). We evaluate the performance for images on both linear and logarithmic intensity scales and discuss their advantages and disadvantages. We propose the use of the STdOCT approach as it offers the best advantages. Applications to in vivo imaging and speckle reduction are presented.

Low-loss high-speed speckle reduction using a colloidal dispersion

Abstract: We present a simple and robust approach to reduce laser speckle, which has limited the adoption of lasers in imaging and display applications. We use colloidal solutions that can quickly reduce speckle contrast due to the Brownian motion of the scattering particles. The high insertion loss associated with propagation through a colloidal solution was overcome by using white paint to cover the sides of the cuvette and an optical fiber to deliver the laser light deep into the colloidal solution, enabling transmission greater than 90%. The diffused laser output followed a Lambertian distribution and produced speckle contrast below 4% at an integration time of 129 μs. The ability for colloidal solutions to achieve fast speckle reduction without power consumption while maintaining high transmission, low cost, a compact size, and a long lifetime makes our approach useful for a wide range of laser imaging and projection applications.

High-Frame-Rate Ultrasound Color-Encoded Speckle Imaging of Complex Flow Dynamics

Abstract: Realization of flow imaging at high frame rates is essential to the visualization of complex flow patterns with fast-changing spatiotemporal dynamics. In this study, we present an experimental demonstration of a novel ultrasound-based high-frame-rate flow visualization technique called color-encoded speckle imaging (CESI), which depicts flow information in a hybrid form comprising flow speckle pattern and color-encoded velocity mapping. This technique works by integrating two key principles: (i) using broad-view data acquisition schemes like plane wave compounding to obtain image data at frame rates well beyond the video display range and (ii) deriving and displaying both flow speckles and velocity estimates from the acquired broad-view image data. CESI was realized on a channel-domain ultrasound imaging research platform, and its performance was evaluated in the context of monitoring complex flow dynamics inside a carotid bifurcation flow phantom with 25% eccentric stenosis at the inlet of the internal carotid artery. Results show that, using an imaging frame rate of 2000 frames per second (based on plane wave compounding with five steering angles), CESI can effectively render flow acceleration and deceleration with visual continuity. It is also effective in depicting how stenosis-related flow disturbance events, such as flow jet formation and post-stenotic flow recirculation, evolve spatiotemporally over a pulse cycle. We anticipate that CESI can represent a rational approach to rendering flow information in ultrasound-based vascular diagnoses.

Digital holographic interferometry with CO2 lasers and diffuse illumination applied to large space reflectors metrology

Abstract: Digital holographic interferometry in the long-wave infrared domain has been developed by combining a CO2 laser and a microbolometer array The long wavelength allows large deformation measurements, which are of interest in the case of large space reflectors undergoing thermal changes when in orbit We review holography at such wavelengths and present some specific aspects related to this spectral range on our measurements For the design of our digital holographic interferometer, we studied the possibility of illuminating specular objects by a reflective diffuser We discuss the development of the interferometer and the results obtained on a representative space reflector, first in the laboratory and then during vacuum cryogenic test

Speckle reduction of retinal optical coherence tomography based on contourlet shrinkage

Abstract: Speckle reduction of retinal optical coherence tomography (OCT) images helps the diagnosis of ocular diseases. In this Letter, we present a speckle reduction method based on shrinkage in the contourlet domain for retinal OCT images. The algorithm overcomes the disadvantages of the wavelet shrinkage method, which lacks directionality and anisotropy. The trade-off between speckle reduction and edge preservation is controlled by a single adjustable parameter, which determines the threshold in the contourlet domain. Results show substantial reduction of speckle noise and enhanced visualization of layer structures as demonstrated in the image of the central fovea region of the human retina. It is expected to be utilized in a wide range of biomedical imaging applications.

Dual-wavelength laser speckle imaging to simultaneously access blood flow, blood volume, and oxygenation using a color CCD camera

Abstract: We developed a dual-wavelength laser speckle imaging system using a single industrial-grade color CCD camera with Bayer filters to simultaneously image changes in blood flow, blood volume, and oxygenation. One frame of a color image recorded with dual-wavelength laser illumination provides not only the intensity fluctuation of the speckle pattern, but also the dual-wavelength optical reflectance signal. The method was validated using a tissue phantom and cuff ischemia experiments in the human arm. This system achieves complete time synchronization, unlike conventional time-sharing systems. Compared with a multicamera system, it also avoids the problem of image registration and can be less expensive.

Speckle interferometry of nearby multiple stars. V. 2002–2006 positional measurements

Abstract: We present the measurements of positional parameters of 194 nearby binary stars performed at the 6-m BTA telescope of the SAO RAS from 2002 through 2006 applying the speckle interferometric technique. The observations were conducted at central filter wavelengths ranging from 545 to 800 nm using a speckle interferometer equipped with a fast CCD and a three-stage image intensifier. A significant part of the observed systems (80 stars) are pairs, the duality of which was discovered or suspected from the Hipparcos satellite observations. The remaining stars are visual binaries and interferometric binary systems with orbital motion period estimates from several to tens of years, as well as the pairs with slow relative motion of the components, used for gaging the image scales and positional angle calibrations.

Effects of speckle/pixel size ratio on temporal and spatial speckle-contrast analysis of dynamic scattering systems: Implications for measurements of blood-flow dynamics

Abstract: Laser Speckle Contrast Imaging (LSCI) is an optical technique used to generate blood flow maps with high spatial and temporal resolution. It is well known that in LSCI, the speckle size must exceed the Nyquist criterion to maximize the speckle's pattern contrast. In this work, we study experimentally the effect of speckle-pixel size ratio not only in dynamic speckle contrast, but also on the calculation of the relative flow speed for temporal and spatial analysis. Our data suggest that the temporal LSCI algorithm is more accurate at assessing the relative changes in flow speed than the spatial algorithm.

Dealing With Speckle Effects in Self-Mixing Interferometry Measurements

Abstract: An analysis of speckle effects and the techniques to overcome them in self-mixing interferometry signals is presented. We characterize the effect of surface roughness and laser spot size on the speckle modulation of the signal, and then propose two simple experimental approaches to overcome the amplitude fading induced by the speckle effect. Unlike the techniques proposed until now, our first approach uses an adaptive optical element in the form of a voltage-programmable liquid lens, which adaptively changes its focal length to modify the speckle pattern. Our second approach combines two laser signals which present different performance parameters. By using any of these simple methods, the introduction of inaccuracies in the measurement process due to speckle is avoided.

From speckle pattern photography to digital holographic interferometry [Invited]

Abstract: Speckles are inherently an interference phenomenon produced when an optically rough surface or a turbulent medium introduces some degree of randomness to a reflected or a transmitted electromagnetic field. Speckles are often nuisance in coherent image formation. Speckle patterns are however a useful tool for displacement and deformation as well as vibration and stress analysis. The development of speckle photography to speckle interferometry and digital holographic interferometry is described in this paper.

Laser speckle imaging based on intensity fluctuation modulation

Abstract: We present a method, intensity fluctuation modulation (IFM), to obtain a full-field laser speckle microvessel image. Different from laser speckle contrast analysis, IFM imaging is insensitive to flow velocity and can be used to reconstruct microvessel images with higher spatial resolution and SNR. An in vivo animal experiment on a mouse pinna is conducted to demonstrate that IFM imaging is capable of achieving laser speckle microangiography.

Mobile speckle interferometer in the long-wave infrared for aeronautical nondestructive testing in field conditions

Abstract: We present the development of a speckle interferometer based on a CO 2 laser and using a thermal infrared camera based on an uncooled microbolometer array It is intended to be used for monitoring deformations as well as detecting flaws in aeronautical composites, with a smaller sensitivity to displacement compared to an equivalent system using visible (VIS) lasers Moreover the long wavelength allows working with such interferometers outside the laboratory A mobile system has been developed on the basis of previous laboratory developments Then it is validated in a variety of industrial nondestructive testing applications in field working conditions

Cost-efficient speckle interferometry with plastic optical fiber for unobtrusive monitoring of human vital signs

Abstract: A cost-efficient plastic optical fiber (POF) system for unobtrusive monitoring of human vital signs is presented. The system is based on speckle interferometry. A laser diode is butt-coupled to the POF whose exit face projects speckle patterns onto a linear optical sensor array. Sequences of acquired speckle images are transformed into one-dimensional signals by using the phase-shifting method. The signals are analyzed by band-pass filtering and a Morlet-wavelet-based multiresolutional approach for the detection of cardiac and respiratory activities, respectively. The system is tested with 10 healthy nonhospitalized persons, lying supine on a mattress with the embedded POF. Experimental results are assessed statistically: precisions of 98.8% ± 1.5% and 97.9% ± 2.3%, sensitivities of 99.4% ± 0.6% and 95.3% ± 3%, and mean delays between interferometric detections and corresponding referential signals of 116.6 ± 55.5 and 1299.2 ± 437.3 ms for the heartbeat and respiration are obtained, respectively.

Simultaneous measurement of the microscopic dynamics and the mesoscopic displacement field in soft systems by speckle imaging.

Abstract: The constituents of soft matter systems such as colloidal suspensions, emulsions, polymers, and biological tissues undergo microscopic random motion, due to thermal energy. They may also experience drift motion correlated over mesoscopic or macroscopic length scales, e.g. in response to an internal or applied stress or during flow. We present a new method for measuring simultaneously both the microscopic motion and the mesoscopic or macroscopic drift. The method is based on the analysis of spatio-temporal cross-correlation functions of speckle patterns taken in an imaging configuration. The method is tested on a translating Brownian suspension and a sheared colloidal glass.

Rapid multiexposure in vivo brain imaging system using vertical cavity surface emitting lasers as a light source

Abstract: We demonstrate an imaging technique implementing vertical cavity lasers with extremely low transient times for a greatly simplified realization of a multiexposure laser speckle contrast imaging system. Data from multiexposure laser speckle imaging was observed to more closely agree with absolute velocity measurements using time of flight technique, when compared to long-exposure laser speckle imaging. Furthermore, additional depth information of the vasculature morphology was inferred by accounting for the change in the static scattering from tissue above vessels with respect to the total scattering from blood flow and tissue.

Quantitative, depth-resolved determination of particle motion using multi-exposure, spatial frequency domain laser speckle imaging

Abstract: Laser Speckle Imaging (LSI) is a simple, noninvasive technique for rapid imaging of particle motion in scattering media such as biological tissue. LSI is generally used to derive a qualitative index of relative blood flow due to unknown impact from several variables that affect speckle contrast. These variables may include optical absorption and scattering coefficients, multi-layer dynamics including static, non-ergodic regions, and systematic effects such as laser coherence length. In order to account for these effects and move toward quantitative, depth-resolved LSI, we have developed a method that combines Monte Carlo modeling, multi-exposure speckle imaging (MESI), spatial frequency domain imaging (SFDI), and careful instrument calibration. Monte Carlo models were used to generate total and layer-specific fractional momentum transfer distributions. This information was used to predict speckle contrast as a function of exposure time, spatial frequency, layer thickness, and layer dynamics. To verify with experimental data, controlled phantom experiments with characteristic tissue optical properties were performed using a structured light speckle imaging system. Three main geometries were explored: 1) diffusive dynamic layer beneath a static layer, 2) static layer beneath a diffuse dynamic layer, and 3) directed flow (tube) submerged in a dynamic scattering layer. Data fits were performed using the Monte Carlo model, which accurately reconstructed the type of particle flow (diffusive or directed) in each layer, the layer thickness, and absolute flow speeds to within 15% or better.

Simultaneous measurement of the microscopic dynamics and the mesoscopic displacement field in soft systems by speckle imaging

Abstract: The constituents of soft matter systems such as colloidal suspensions, emulsions, polymers, and biological tissues undergo microscopic random motion, due to thermal energy. They may also experience drift motion correlated over mesoscopic or macroscopic length scales, \textit{e.g.} in response to an internal or applied stress or during flow. We present a new method for measuring simultaneously both the microscopic motion and the mesoscopic or macroscopic drift. The method is based on the analysis of spatio-temporal cross-correlation functions of speckle patterns taken in an imaging configuration. The method is tested on a translating Brownian suspension and a sheared colloidal glass.

Speckle suppression by doubly scattering systems.

Abstract: Speckle suppression in a two-diffuser system is examined. An analytical expression for the speckle space-time correlation function is derived, so that the speckle suppression mechanism can be investigated statistically. The grain size of the speckle field illuminating the second diffuser has a major impact on the speckle contrast after temporal averaging. It is shown that, when both the diffusers are rotating, the one with the lower rotating speed determines the period of the speckle correlation function. The coherent length of the averaged speckle intensity is shown to equal the mean speckle size of the individual speckle pattern before averaging. Numerical and experimental results are presented to verify our analysis in the context of speckle reduction.

Extendable, miniaturized multi-modal optical imaging system: cortical hemodynamic observation in freely moving animals

Abstract: Observation of brain activities in freely moving animals has become an important approach for neuroscientists to understand the correlation between brain function and behavior. We describe an extendable fiber-optic-based multi-modal imaging system that can concurrently carry out laser speckle contrast imaging (LSCI) of blood flow and optical intrinsic signal (OIS) imaging in freely moving animals, and it could be extended to fluorescence imaging. Our imaging system consists of a multi-source illuminator, a fiber multi-channel optical imaging unit, and a head-mounted microscope. The imaging fiber bundle delivers optical images from the head-mounted microscope to the multi-channel optical imaging unit. Illuminating multi-mode fiber bundles transmit light to the head-mounted microscope which has a mass of less than 1.5 g and includes a gradient index lens, giving the animal maximum movement capability. The internal optical components are adjustable, allowing for a change in magnification and field of view. We test the system by observing hemodynamic changes during cortical spreading depression (CSD) in freely moving and anesthetized animals by simultaneous LSCI and dual-wavelength OIS imaging. Hemodynamic parameters were calculated. Significant differences in CSD propagation durations between the two states were observed. Furthermore, it is capable of performing fluorescence imaging to explore animal behavior and the underlying brain functional activity further.

Correcting speckle contrast at small speckle size to enhance signal to noise ratio for laser speckle contrast imaging.

Abstract: In laser speckle contrast imaging, it was usually suggested that speckle size should exceed two camera pixels to eliminate the spatial averaging effect. In this work, we show the benefit of enhancing signal to noise ratio by correcting the speckle contrast at small speckle size. Through simulations and experiments, we demonstrated that local speckle contrast, even at speckle size much smaller than one pixel size, can be corrected through dividing the original speckle contrast by the static speckle contrast. Moreover, we show a 50% higher signal to noise ratio of the speckle contrast image at speckle size below 0.5 pixel size than that at speckle size of two pixels. These results indicate the possibility of selecting a relatively large aperture to simultaneously ensure sufficient light intensity and high accuracy and signal to noise ratio, making the laser speckle contrast imaging more flexible.

Role of contrast and fractality of laser speckle image in assessing flow velocity and scatterer concentration in phantom body fluids

Abstract: Blood flow velocity and red blood cell concentration are of vital importance in assessing tissue microcirculation. Laser speckle contrast analysis is being considered as a promising tool in the qualitative assessment of flow velocity as well as scatterer concentration in different body fluids, though the quantification part still remains challenging. The fractal-based spatial correlation analysis of speckle flow images along with the corresponding contrast analysis for the quantitative assessment of flow and scatterer concentration is investigated. In this study, phantom body fluid solution (intralipid 20%) of different concentrations is pumped at different flow rates through the designed flow channel using a syringe pump and the corresponding speckle images are acquired. The fractality of the acquired speckle images in response to the changes in concentration of the fluid as well as the variations in fluid flow is analyzed along with the corresponding contrast-based analysis. Following this qualitative analysis, an experimental model is attempted toward quantification of these parameters from a single acquired speckle image by considering the contrast and fractality changes together.

Full speckle suppression in laser projectors using two Barker code-type diffractive optical elements.

Abstract: The mathematical model of a speckle-suppression method based on two Barker code-type diffractive optical elements (DOEs) moving in orthogonal directions is developed. The analytic formulae for speckle suppression efficiency are obtained. The model indicates that the one pair of DOEs can be used for laser beams of different colors. The speckle contrast is not dependent on the distance from the viewer to the screen until the distance decreases below the distance where the spatial resolution of the eye on the screen is less than the length of the image of the DOE structure period on the screen. The analysis of the simulated results demonstrates that the method can decrease the speckle contrast to less than 5%, which is below human eye sensitivity, with an optical efficiency greater than 90%.

Application of two oriented partial differential equation filtering models on speckle fringes with poor quality and their numerically fast algorithms.

Abstract: In this paper, we are concerned with denoising in experimentally obtained electronic speckle pattern interferometry (ESPI) speckle fringe patterns with poor quality. We extend the application of two existing oriented partial differential equation (PDE) filters, including the second-order single oriented PDE filter and the double oriented PDE filter, to two experimentally obtained ESPI speckle fringe patterns with very poor quality, and compare them with other efficient filtering methods, including the adaptive weighted filter, the improved nonlinear complex diffusion PDE, and the windowed Fourier transform method. All of the five filters have been illustrated to be efficient denoising methods through previous comparative analyses in published papers. The experimental results have demonstrated that the two oriented PDE models are applicable to low-quality ESPI speckle fringe patterns. Then for solving the main shortcoming of the two oriented PDE models, we develop the numerically fast algorithms based on Gauss–Seidel strategy for the two oriented PDE models. The proposed numerical algorithms are capable of accelerating the convergence greatly, and perform significantly better in terms of computational efficiency. Our numerically fast algorithms are extended automatically to some other PDE filtering models.

Retrieving controlled motion parameters using two speckle pattern analysis techniques: spatiotemporal correlation and the temporal history speckle pattern

Abstract: This paper presents simulation of speckle activity through controlling a moving plate. We present two procedures to extract the initial movement frequency and amplitude, either through correlation calculus or through processing the temporal history of the speckle pattern. We compare and discuss these two methods in terms of efficiency and the ability to retrieve motion parameters. The correlation technique seems to be more suitable for monitoring biospeckle activity as it provides more reliable parameter estimation than the temporal history of the speckle pattern. The evolution of temporal history of the speckle pattern parameters and their response sensibility with amplitude and frequency variations have been studied and quantified. Briers contrast appears to depend only on movement amplitude, whereas inertia moment varies with amplitude and frequency.

Optimal speckle suppression in laser projectors using a single two-dimensional Barker code diffractive optical element

Abstract: An effective method of speckle suppression using one 2D diffractive optical element (DOE) moving with constant velocity based on the periodic Barker code sequence is developed. We prove that this method has the same optical parameters as the method based on two 1D Barker code DOEs stretched and moving in orthogonal directions. We also show that DOE movement in a special direction allows the full numerical aperture of the objective lens to be used for speckle averaging by angle diversity. It is found that the 2D DOE based on a Barker code of length of 13 allows the speckle contrast to be decreased below the sensitivity of the human eye with optical losses of less than 10%.