scispace - formally typeset
Search or ask a question
Topic

Sperm motility

About: Sperm motility is a research topic. Over the lifetime, 13874 publications have been published within this topic receiving 416587 citations. The topic is also known as: sperm movement & GO:0097722.


Papers
More filters
Journal ArticleDOI
TL;DR: In this article, a retrospective analysis of 2,245 cycles of therapeutic donor IUIs were initially studied; 1,147 cycles that met selection criteria were used in this report to determine if conventional sperm parameters, specific characteristics of sperm motion determined by computer-aided semen analysis (CASA), sperm penetration assay (SPA), and spontaneous acrosome reaction assay could best predict fertility outcome after intrauterine insemination (IUI) from frozen donor sperm.

99 citations

Journal ArticleDOI
04 Jan 1980-Science
TL;DR: Results provide direct evidence for the existence of sperm plasma membrane receptors for the zona pellucida of the pig.
Abstract: Freshly ejaculated, noncapacitated boar sperm bind rapidly and in large numbers to pig egg zona pellucida in vitro. In the present study, the number of sperm bound decreased sharply when sperm motility was lowered by energy poisons or by reducing the temperature. Highly motile sperm from humans, guinea pigs, and rats, added at concentrations ten times higher than control sperm, did not bind to the porcine zona. At the same high concentration, a small number of hamster and bull sperm bound to the zona. Binding of boar sperm to the zona pellucida was blocked almost completely by diluted whole antiserum to sperm plasma membranes and by univalent (Fab) antibody to these membranes. When antibody to sperm plasma membrane was first absorbed with plasma membrane vesicles, sperm binding was not inhibited. These results provide direct evidence for the existence of sperm plasma membrane receptors for the zona pellucida of the pig.

99 citations

Journal ArticleDOI
TL;DR: A study was conducted to determine an optimum technique for semen cryopreservation and the biological competence of frozen-thawed ferret spermatozoa and sperm motility and normal acrosomal ratings were highest using the PDV diluent, the pelleting method and thawing at 37 degrees C.
Abstract: A study was conducted to determine an optimum technique for semen cryopreservation and the biological competence of frozen-thawed ferret spermatozoa. Fifty-two fresh electroejaculates from 4 males were evaluated for sperm percentage motility, forward progressive motility, motility index (SMI) and acrosomal integrity. To determine the optimum temperature for maintaining sperm motility in vitro and the influence of glycerol on sperm motility, seminal aliquants were diluted in TEST diluent (containing either 0 or 4% glycerol) and maintained at 25 degrees or 37 degrees C. For cryopreservation, semen was diluted in each of 3 cryodiluents (TEST, PDV, BF5F), cooled for 30 min at 5 degrees C and pelleted on solid CO2 or frozen in 0.25 ml straws (20 degrees C/min to -100 degrees C). Following thawing, SMI and acrosomal integrity were determined. Ten females with maximum vulval swelling were given 90 i.u. human chorionic gonadotrophin and laparoscopically inseminated in utero with spermatozoa previously frozen using the optimum diluent and freeze-thaw method. The maintenance temperature of 25 degrees C was superior (P less than 0.05) to 37 degrees C for sustaining sperm motility, and glycerol did not influence (P greater than 0.05) motility for up to 11 h of culture. After thawing, motile spermatozoa were recovered in all treatment groups, but sperm motility and normal acrosomal ratings were highest using the PDV diluent, the pelleting method and thawing at 37 degrees C (P less than 0.05). Seven of the 10 ferrets (70%) inseminated with spermatozoa frozen by this approach became pregnant and produced 31 kits (mean litter size 4.4; range 1-9 kits).(ABSTRACT TRUNCATED AT 250 WORDS)

99 citations

Journal ArticleDOI
TL;DR: It is demonstrated that the highly localized pattern of PKA seen in mature sperm is not essential for motility or fertilization.

99 citations

Journal ArticleDOI
TL;DR: The results support the hypothesis that quiescence is induced by a rise in intracellular Ca2%, perhaps as a consequence of a membrane depolarization, and that it is similar to the arrest response in cilia.
Abstract: Sperm of the sea urchin Tripneustes gratilla repeatedly start and stop swimming when suspended in seawater and observed by dark-field microscopy. While in the quiescent state, which usually lasts about a second, the sperm assume s shape resembling a cane, with a sharp bend of approximately 3.4 rad in the proximal region of the flagellum and very little curvature in the rest of the flagellum except for a slight curve near the tip. The occurrence of quiescence requires the presence of at least 2 mM Ca2+ in the seawater, and the percentage of sperm quiescent at any one time increases substantially when the sperm are illuminated with blue light. With intense illumination, close to 100% of the sperm become quiescent, and this percentage decreases gradually to approximately 0.3% over a 10(4)-fold decrease in light intensity. An increased concentration of K+ in the seawater also increases the percentage of quiescence, with a majority of the sperm being quiescent in seawater containing 80 mM KCl. The induction of quiescence by light or by increased KCl is completely inhibited by 10 micrometers chlorpromazine, and approximately 90% inhibited by 1 mM procaine or sodium barbital. Sperm treated with the divalent-cation ionophore A23187 swim quite normally, although for a relatively short period, in artificial seawater lacking divalent cations, but are abruptly arrested upon addition of 0.04--0.2 mM free Ca2%. The flagellar waveform of these arrested sperm is almost identical to that of light-induced quiescence in the live sperm. The results support the hypothesis that quiescence is induced by a rise in intracellular Ca2%, perhaps as a consequence of a membrane depolarization, and that it is similar to the arrest response in cilia.

99 citations


Network Information
Related Topics (5)
Sperm
43.4K papers, 1.3M citations
95% related
Luteinizing hormone
23.9K papers, 756K citations
85% related
Testosterone
23.2K papers, 808K citations
82% related
Androgen
18.9K papers, 798.4K citations
80% related
Prolactin
22.3K papers, 609.5K citations
80% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023383
2022912
2021582
2020616
2019552
2018576