scispace - formally typeset
Search or ask a question
Topic

Sperm plasma membrane

About: Sperm plasma membrane is a research topic. Over the lifetime, 1016 publications have been published within this topic receiving 49964 citations.


Papers
More filters
01 Jan 2008
TL;DR: The aim of this study was to determine the relationship between ROS, TAC and DNA damage on spermatozoon viability in relation to age and found no significant associations.
Abstract: Male infertility is now recognised as a significant factor in couples having difficulty conceiving. The impact of maternal age has long been known as a limiting factor, however recent research indicates that advancing paternal age can also negatively impact on a couple's chances of conception. One of the major contributing causes of male infertility has now been linked to spermatozoa exposure to reactive oxygen species (ROS). Such exposure induces oxidative stress when coupled with reduced total antioxidant capacity (TAC). Measures of both ROS and TAC are used as tests of oxidative stress status (OSS) which are used together to give further insight into male fertility status. Current research has revealed that the level of DNA fragmentation increases with age and, due to their composition, the sperm plasma membrane, DNA double helix and single stranded DNA are highly susceptible to ROS attack. ROS cause high levels of lipid peroxidation (LPO) that ultimately damages the plasma membrane and interferes with its vital functions. ROS also readily attack the purine and pyrimidine bases of DNA resulting in DNA damage. It is hypothesized that advancing paternal age will impact negatively of levels of ROS, TAC and DNA fragmentation in ageing men. The aim of this study was to determine the relationship between ROS, TAC and DNA damage on spermatozoon viability in relation to age. Ejaculated semen samples from 54 men undergoing infertility assessment were collected and divided into men aged ~40 years (n=16) and men aged ::;;39 years (n=38). Samples were examined for their level of ROS and TAC as a possible indication of oxidative stress status. Samples were also assessed for DNA fragmentation and damage using TUNEL. Statistical analysis consisted of independent t-tests, chi-square tests and multivariate logistic regression. Analysis found no significant associations between ROS, TAC, TUNEL and age. Significant differences were observed between abnormal sperm motility and age (p<0.05; t-test and

1 citations

Journal ArticleDOI
TL;DR: In this paper, cholesterol-loaded cyclodextrins (CLC) were used to stabilize the plasma membranes of sperm sexed by adding cholesterol loaded cyclodesxtrin post-thaw and consequently extend their functionality, increase their ability to fertilize oocytes and produce embryos in vitro.
Abstract: Bovine sperm have a low cholesterol/phospholipids ratio in the sperm plasma membrane This characteristic is important due to cholesterol plays a key role in maintaining the structure and function of sperm membrane after freezing, and therefore, increasing sperm survival This situation could be amplified in case of sexed semen The aim of this study is to stabilize the plasma membranes of sperm sexed by adding cholesterol-loaded cyclodextrins (CLC) post-thaw and consequently extend their functionality, increase their ability to fertilize oocytes and produce embryos in vitro Sexed sperm were treated with CLC at different concentrations and incubated for 15 minutes before evaluating different motility parameters by a computerized system (CASA) and fertilize oocytes in vitro Sperm treated with 3 mg of methyl-β-cyclodextrin saturated cholesterol for each 120x106 of sperm, showed higher motility parameters and remained high during the analyzed period The concentration of 3 mg of CLC also increased cleavage rate, early (MOR/eBL), late (BL) and total embryo development

1 citations

01 Jan 2008
TL;DR: The results suggest that semen cryopreservation could enhance the sperm ability in picking up foreign DNA as well as reduce the integrity of sperm plasma membrane and enhanced the sperm genomic DNA strand breakage significantly.
Abstract: The efficiency and the ability of fresh and frozen-thawed sperm in picking up exogenous DNA were investigated in this study.The fresh and frozen-thawed sperm was incubated with linearized end-labeled pEGFP-N_1 plasmid DNA,and detected by in situ hybridization method.The in vitro producted embryos were screened by PCR assay.The ultrastructure of activated spermatozoa were observed by transmission electron microscope(TEM),and the integrity of sperm plasma membrane was evaluated with a combination of fluorescent probes-carboxifluorescein diacetate and propidium iodide(PI).The sperm genomic DNA damage was determined by single cell gel electricity assay(SCGE)methods.The results showed that the frozen-thawed treated goat sperm cells were more efficient and more reliable than untreated sperm in picking up exogenous DNA and subsequently internalizing the DNA into sperm nuclei(81.60%±16.59% vs 32.95%±2.93%,t=4.873,P=0.003;41.80%±6.26% vs 27.89%±8.64%,t=2.634,P=0.039).The exogenous DNA retained its integrity in sperm as demonstrated with PCR and Southern Blotting assay methods.The rate of transgenesis embryos produced by exogenous DNA incubated sperm was enhanced significantly(45.45%±10.87% vs 24.44%±6.06%,t=1.750,P=0.013).The semen cryopreservation reduced the integrity of sperm plasma membrane(8.34%±4.21% vs 65.67%±6.46%,t=12.492,P0.001)and enhanced the sperm genomic DNA strand breakage significantly.These results suggest that semen cryopreservation could enhance the sperm ability in picking up foreign DNA.The cryopreservation-induced changes in the sperm plasma membrane facilitate the internalization exogenous DNA.

1 citations

01 Jan 2014
TL;DR: The findings indicated that the decabromodiphenyl ether led to damages in the plasma membrane, mitochondria and flagella of the sperm, including plasma membrane rupture, mitochondrial- vacuole like change, and deciduous and accumulated flaglla and disintegrated axoneme microtubules.
Abstract: The effects of different concentrations of decabromodiphenyl ether on ultrastucture of sperm in Pacific cod Gadus macrocephalus were studied by scanning electron microscopy(SEM) and transmission electron microscopy(TEM). The results showed that there was no significant difference in ultrastructure of sperm between the Pacific cod in the control group and the Pacific cod exposed to 0. 5,5,50,and 500 ng / L decabromodiphenyl ethers. However,the Pacific cod exposed to 5000 ng / L had the sperm with significant changes in the ultrastructure compared with the fish in the control group. The SEM revealed that white crystalline lesions on the sperm nucleus surface and ruptured nuclei were observed in sperm of the cod exposed to 5000 ng / L for 8 h and 12 h,even though there was no significant change in the ultrastructure of sperm in the Pacific cod exposed for 2 h and 4 h. Under the TEM,no significant change in the sperm morphology was found in the cod exposed to 5000 ng / L for 2 h compared with the fish in the control group. In 5000 ng / L 4 h exposure,however,the expansion of sperm plasma membrane and shifting of mitochondria were observed,and the ruptured plasma membrane,broken,even dissolved,mitochondrial cristae,and vacuolar shallow changes in the matrix were found in the cod exposed to 5000 ng / L for 8 h. The cod exposed to 5000 ng / L for 12 h had deciduous and accumulated flagella in abundance,some disintegrated microtubules and mitochondrial cristae,even disappearance. The findings indicated that the decabromodiphenyl ether led to damages in the plasma membrane,mitochondria and flagella of the sperm,including plasma membrane rupture,mitochondrial- vacuole like change,deciduous and accumulated flagella and disintegrated axoneme microtubules.

1 citations

01 Jan 1991
TL;DR: Two testable hypotheses are formulated that are compatible with the available data: either p95 is itself a protein tyrosine kinase receptor, or p95 serves as a ZP3 receptor and is separate from aprotein tyrosin kinase that is activated during gamete interaction.
Abstract: Although details of the molecular mechanism are not yet clear, considerable evidence suggests thatthe egg-specific extracellularmatrix component ZP3 regulates an essential event of sperm function,the acrosome reaction,Spatialcontrol of this exocytotic event appears to be exerted by immobilization of the triggering ligand, ZP3, in the zona pellucidamatrix surrounding the egg. Our data suggest that the signal transduction pathway in sperm activated by this ligand involves highly conserved components that are involved in many other eukaryotic signalling events, Recent experiments indicate that the murine zona pellucida glycoprotein ZP3 regulates acrosomal exocytosis by aggregating its corresponding receptors (ZP3-Rs) located in the mouse sperm plasma membrane. In other experiments, we have identified a putative ZP3-R of mouse sperm with M, 95000. Indirectimmunofluorescence localizes this ZP3-R, termed p95, to the acrosomal region of the mouse sperm head, which is the anticipated location for ZP3-Rs. Membrane fractionation studies indicate that p95 cofractionates with a plasma membrane-enriched preparation from sperm that contains zona pellucida-receptor activity. In addition to its role as a ZP3-R, p95 also serves as a substrate for a tyrosine kinase in response to zona pellucida binding. On the basis of the data presented here, and borrowing heavily from fmdings for other signalling systems, we have formulated two testable hypotheses that are compatible with the available data: either p95 is itself a protein tyrosine kinase receptor, or p95 serves as a ZP3 receptor and is separate from a protein tyrosine kinase that is activated during gamete interaction, Examination of these hypotheses will allow further exploration of the relationshipbetween sperm binding to the zona pellucida and triggering of the acrosome reaction,

Network Information
Related Topics (5)
Sperm
43.4K papers, 1.3M citations
90% related
Ovulation
18.9K papers, 577.2K citations
85% related
Luteinizing hormone
23.9K papers, 756K citations
83% related
Prolactin
22.3K papers, 609.5K citations
77% related
Infertility
22.3K papers, 545.5K citations
77% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202029
201920
201827
201726