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Sperm plasma membrane

About: Sperm plasma membrane is a research topic. Over the lifetime, 1016 publications have been published within this topic receiving 49964 citations.


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TL;DR: It is necessary specific management in the non-rainy season that thermal stress is not a determining factor in reducing the reproductive quality of buffaloes; it is necessary to use means to improve animal welfare.
Abstract: Awareness of the physiological changes that occur when animals are subjected to climatic changes that are considered stressful is essential to maintain animal welfare and to be able to exploit their reproductive potential efficiently and rationally. The present study was carried out to evaluate climatic variables’ influence on physiological parameters, and Murrah buffalo ejaculates reared in a humid tropical climate in the Amazon. The immediate analyzes pertinent to the physical and morphological characteristics of the ejaculates were carried out and corresponded in the rainy season (RS) volume of 3.4±2.0mL; the mass activity of 4.4±0.5; motility of 80.4±5.6%; vigor of 4.4±0.4; concentration of 657,300±237,865.1 x 106sptz/mL; major defects of 9.0±2.6%; minor defects of 11.2±3.9%; total defects 20.2±5.3% and sperm plasma membrane integrity (SPMI) 84.8±5.6%, whereas in the non-rainy season (nRS), the results were 4.0±2.1mL; the mass activity of 3.0±1.0; motility of 56.2±13.4%; vigor of 3.0±1.0; concentration of 586,000±291,925.9 x 106sptz/mL; major defects of 20.8±9.9%; minor defects of 27.5±6.3%; total defects 48.3±9.3% and SPMI of 57.9±12.4%. Furthermore, a statistical difference (P<0.05) was observed for the parameters mass activity, motility, vigor, major defects, minor defects, total defects, and sperm plasma membrane integrity between both periods. The data on heart frequency, superficial temperature (head, back, groin, and scrotal pouch) showed a statistical difference between both periods (P<0.05). To conclude is necessary specific management in the non-rainy season that thermal stress is not a determining factor in reducing the reproductive quality of buffaloes; it is necessary to use means to improve animal welfare; one alternative is to use baths regularly for these animals or provide constant access to areas of rivers or lakes, as well as shading, preventing the buffaloes from being directly exposed to the unfavorable thermal environment.
Journal ArticleDOI
TL;DR: A functional competence of the AM was supported by the correlation of theAM-expression on the spermatozoal surface after the acrosome reaction with three function tests: the zona-free hamster oocyte penetration test, the in vitro fertilization of human oocytes and the cell attachment assays.
Abstract: Ejaculated spermatozoa and spermatogenic cells express α- and β-chains of β1-, β3- and β4-integrins as well as their ligands laminin and fibronectin. These adhesion molecules (AM) showed an extended intra- and interindividual variation and different patterns of location. The mRNA transcripts of the AM were detectable by nested polymerase chain reaction in the spermatozoa. The conclusion of a functional competence of the AM was supported by the correlation of the AM-expression on the spermatozoal surface after the acrosome reaction with three function tests: (i) the zona-free hamster oocyte penetration (HOP) test, (ii) the in vitro fertilization of human oocytes and (iii) the cell attachment assays. AM labelling was modified by progesterone, human follicular fluid, disintegration of the sperm plasma membrane in seminal plasma, and micro organisms. In contrast, the sperm cryopreservation did barely influence the labelling of spermatozoal AM.
Patent
04 Jan 2017
TL;DR: In this article, a method for improving quality of a mammalian semen sample includes providing an insoluble carrier carrying at least one ligand that binds to an indicator of impaired sperm membrane integrity and/or motility.
Abstract: A method for improving quality of a mammalian semen sample includes providing an insoluble carrier carrying at least one ligand that binds to an indicator of impaired sperm membrane integrity and/or motility, or the insoluble carrier carrying at least one molecule capable of binding to the at least one ligand, and contacting the semen sample and the insoluble carrier to provide a bound sperm cell population and an unbound sperm cell population, The insoluble carrier may be a plurality of beads each carrying the at least one ligand or the at least one molecule. The indicator may be associated with one or both of impaired sperm acrosome and impaired sperm plasma membrane integrity. The ligand may be an antibody. The molecule may be one of streptavidin, protein A, and an anti-ligand antibody. Kits for performing the method are provided.
Journal ArticleDOI
TL;DR: Sperm plasma membrane was damaged in antibodies treated spermatozoa as evidenced by hypoosmotic swelling test and sperm lipid peroxidation reaction.
Abstract: Polyclonal antibodies to intact inhibin (94 amino acids, R-94, 10.5 kDa) and its sequence specific synthetic fragments (R-9, R-17) were evaluated for their effect on various physical and biochemical parameters of sperm function. Intact inhibit had maximum deleterious effect on quantitative motility and mean forward progression of spermatozoa. Antibodies had no effect on sperm fructolysis and sperm nuclear chromatin decondensation reaction. Sperm plasma membrane was damaged in antibodies treated spermatozoa as evidenced by hypoosmotic swelling test and sperm lipid peroxidation reaction.
01 Jan 2005
TL;DR: UPA could induce SC of mice sperm in vitro through the uPAR on its membrane, enhance the capability of egg inducing SC, and promote spermatozoa to fertilize eggs, and enhance the fertility success correspondingly.
Abstract: Objective To explore the role of urokinase-type plasminogen activator(uPA) in precontact sperm-egg communication and fertility of mice in vitro.Methods Firstly, sperm chemotaxis (SC) induced by uPA was assayed by measuring the sperm densities in capillaries with a descending gradient or no gradient of uPA respectively. Secondly, the role of uPAR that exists in sperm plasma membrane in SC was studied by examining the change of sperm density in capillary after incubating spermatozoa with anti-uPAR antibody. Thirdly, SC induced by eggs, which had been treated with uPA, PAI-1 and anti-uPAR beforehand respectively, was assayed to study the role of uPA in PSEC. Lastly, the fertilization capability of spermatozoa treated with uPA was examined by counting the number of fertilized eggs.Results 1)The density of spermatozoa that migrated down the gradient of uPA into the capillary was significantly lower than that into the capillary containing no-gradient uPA. 2) When uPAR of spermatozoa was inhibited by anti-uPAR antibody, the density of spermatozoa that migrated into the capillary with ascending gradient of uPA decreased correspondingly. 3) The density of spermatozoa attracted by eggs, which were treated with uPA beforehand, increased significantly than that of attracted by non-treated eggs. On the contrary, the sperm density decreased correspondingly when the egg was treated with PAI-1. 4) The number of fertilized eggs increased significantly after the spermatozoa used here was treated with uPA beforehand.Conclusion uPA could induce SC of mice sperm in vitro through the uPAR on its membrane, enhance the capability of egg inducing SC, and promote spermatozoa to fertilize eggs. Thus, uPA may act as an attractant in PSEC, increase the chance encounter of spermatozoa and eggs, therefore, enhance the fertility success correspondingly.This study, in some degree, provides an evidence that uPA may be used as a new medicine and diagnostic reagent for male infertility.

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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202029
201920
201827
201726