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Sperm plasma membrane

About: Sperm plasma membrane is a research topic. Over the lifetime, 1016 publications have been published within this topic receiving 49964 citations.


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Journal ArticleDOI
12 Oct 2020
TL;DR: In this article, a pig sperm-egg interaction model was used to examine quantitative details of zona binding and zona penetrating abilities within capacitated sperm populations, and sperm behaviour was found not to accord with generally held beliefs.
Abstract: Much effort is being made to establish relationships between the molecular events that take place in spermatozoa under fertilizing conditions and actual sperm function during fertilization. During capacitation, the process that 'primes' spermatozoa for interaction with the egg, components of the sperm's environment, notably bicarbonate, provoke various specific changes in the architecture and functioning of the sperm plasma membrane in a large number of cells. The individual changes have been found to proceed on different time scales, and may therefore represent sequential stages in the capacitation process. However, each change takes place at different rates in individual cells, revealing considerable functional heterogeneity within the sperm population. Recent work on membrane changes provoked by cooling has indicated similarities with capacitational changes. The effect of cooling may therefore be to induce premature capacitation (and destabilization). Such an effect would greatly compromise sperm fertilizing potential. A pig sperm-egg interaction model was used to examine quantitative details of zona binding and zona penetrating abilities within capacitated sperm populations, and sperm behaviour was found not to accord with generally held beliefs. In particular, individual spermatozoa that have bound to the zona pellucida show great variation in the delay before penetrating: no evidence has been found for a specially competent subgroup. Even in sperm samples incubated to undergo maximal capacitational membrane changes, cells with actual penetrating potential represent less than 15% of the total number that attach initially to the zona pellucida. Thus detection of capacitational membrane changes appears greatly to overestimate zona penetrating capability. Future studies linking sperm membrane characteristics with semen fertility in the field will need to consider differences between in vitro and in vivo conditions. The need for survival in the female tract may require much slower sperm responses than are considered optimal for in vitro fertilization.

62 citations

Journal ArticleDOI
TL;DR: The supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.
Abstract: The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk (group I), DHA-enriched hen egg yolk (group II), normal hen egg yolk with 5 mmol L(-1) of cysteine supplementation (group III) and DHA-enriched hen egg yolk with 5 mmol L(-1) of cysteine supplementation (group IV). The semen was cryopreserved using controlled rate freezer and was thawed at 50 degrees C for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone (group III) improved progressive motility (P 0.05). In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.

62 citations

Journal ArticleDOI
TL;DR: It would appear that high levels of CHOL and monounsaturated fatty acids provided the spermatozoa with increased resistance to cryopreservation damage.
Abstract: The effects of dietary lipid from four experimental diets on the fatty acid (FA) composition and cholesterol (CHOL) content of spermatozoa and spermatozoal plasma membranes and their consequences for sperm viability after cryopreservation were evaluated in rainbow trout Oncorhynchus mykiss (Walbaum). The four sources of lipid were herring oil (adequate n-3 FA), menhaden oil (high n-3 FA), safflower oil (high n-6 FA) or tallow (high saturated FA), and they comprised 12% of the total diet. Spermatozoa from fish fed the tallow diet had significantly (P < 0.05) higher CHOL levels than spermatozoa from the fish fed the other diets. The spermatozoal plasma membranes from fish fed the tallow diet had significantly (P < 0.05) higher CHOL and monounsaturated fatty acid levels than those from fish fed the menhaden or safflower oil diets, but were not different from membranes of fish fed the herring oil diet. Cryopreserved spermatozoa from fish fed the tallow or herring oil diets exhibited less membrane damage (P < 0.05) and produced a higher percentage (P < 0.05) of eyed embryos compared with spermatozoa from the menhaden or safflower oil-fed fish. Therefore, it would appear that high levels of CHOL and monounsaturated fatty acids provided the spermatozoa with increased resistance to cryopreservation damage.

62 citations

Journal ArticleDOI
TL;DR: Christen as discussed by the authors explored the relationship between the plasma membrane potential and activation of sperm motility and respiration, or induction of the acrosome reaction, was explored in sperm of the sea urchin Strongylocentrotus purpuratus.

62 citations

Journal ArticleDOI
TL;DR: It is suggested that egg yolk protects spermatozoa during chilling and freezing by its attachment to the sperm plasma membrane by competing with ANS for sites on the plasma membrane.
Abstract: The flourescent membrane marker, 1-anilinoaphtalene-8-sulphonate (ANS) was used to investigate the attachment of egg-yolk to the plasma membranes of ram spermatozoa. The degree of fluorescence was assessed using a subjective scoring system. It was found that egg yolk competes with ANS for sites on the plasma membrane. When the diluent contained 10% egg yolk, no ANS could be detected on the membranes. Egg yolk attached to the plasma membrane could be removed by washing twice with a yolk-free diluent. Loss of sperm motility in the presence of ANS was observed but some spermotozoa remained motile after incubation at 37 degrees C for 15 min with 2mM-ANS. Egg yolk protected spermatozoa against this loss of motility. It is suggested that egg yolk protects spermatozoa during chilling and freezing by its attachment to the sperm plasma membrane.

62 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202029
201920
201827
201726