Topic
Sperm washing
About: Sperm washing is a research topic. Over the lifetime, 1006 publications have been published within this topic receiving 26916 citations.
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TL;DR: In this paper, the incidence of reactive oxygen species formation in whole semen and in washed spermatozoa was studied and the effect of the type of sperm washing was also investigated.
593 citations
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TL;DR: The results suggest that when poor-quality semen samples are used for ICSI, there is a greater likelihood that some sperm selected for injection, despite appearing normal, contain fragmented DNA.
485 citations
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TL;DR: The results showed that there was no important influence from either the type or the extent of sperm impairment on the outcome of ICSI, and the only ultimate criterion for successful ICSi is the presence of at least one living spermatozoon per oocyte in the pellet of the treated semen sample used for micro-injection.
Abstract: High success rates have been reported for the use of intracytoplasmic sperm injection (ICSI) in alleviating essentially andrological infertility. However, neither the relationship between any of the sperm parameters and the result of ICSI nor the minimal sperm requirements for ICSI have been investigated so far. In this paper, our objective was therefore to study the relationship between three basic sperm parameters (total sperm count, sperm motility and morphology) and the outcome of ICSI by retrospective analyses of fertilization, embryo development and pregnancy rates in 966 micro-injection cycles, performed with ejaculated semen. The results showed that there was no important influence from either the type or the extent of sperm impairment on the outcome of ICSI. Even in the most extreme cases of male-factor infertility, where cryptozoospermia or total astheno- or total teratozoospermia was diagnosed in the initial semen sample, high fertilization and pregnancy rates were obtained by ICSI. Only one condition had a strongly negative influence on the result of ICSI: where an immotile (presumably dead) spermatozoon was injected into the oocyte. Thus the only ultimate criterion for successful ICSI is the presence of at least one living spermatozoon per oocyte in the pellet of the treated semen sample used for micro-injection.
466 citations
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TL;DR: A review is given on the use of frozen-thawed semen for artificial insemination (AI) in spontaneous and induced oestrus and factors influencing the fertility.
366 citations
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TL;DR: Intracytoplasmic sperm injection can be used successfully to treat couples who have failed IVF or who have too few spermatozoa for conventional methods of in vitro insemination, and sperm parameters do not clearly affect the outcome of this technique.
342 citations