Topic
Staphylococcus arlettae
About: Staphylococcus arlettae is a research topic. Over the lifetime, 41 publications have been published within this topic receiving 1114 citations.
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TL;DR: An extremely halotolerant bacterium designated as HPSSN35C was isolated from saline soil of Dwarka beach, India and exhibited growth over a wide range of NaCl in medium varying from 0 to 6’M, and produced peach–pink pigment above ∼1.3 M NaCl.
Abstract: An extremely halotolerant bacterium designated as HPSSN35C was isolated from saline soil of Dwarka beach, India. It exhibited growth over a wide range of NaCl in medium varying from 0 to 6 M. The isolate produced peach-pink pigment above ∼1.3 M NaCl. The culture was characterized using biochemical tests, bioMerieux Staph identification kit, API ID32 Staph system, and Biolog. Due to slow growth and extreme salt tolerance no ID was obtained in Biolog. Antibiotic sensitivity to various antibiotics was tested. Phenotypic characterization showed that it belonged to the novobiocin resistant staphylococci group. Analysis of 16S rRNA gene sequence comparison of 1452 base pairs showed that isolate is closely related to Staphylococcus saprophyticus group with close relationship to Staphylococcus arlettae (99% similarity). The halotolerant S. arlettae described in literature till date have been reported to tolerate 4.5 M NaCl and produce white to yellow pigment. The present study reports for the first time extremely halotolerant S. arlettae exhibiting growth up to ∼6 M NaCl and producing peach-pink pigment above ∼1.3 M NaCl.
11 citations
TL;DR: The potential for in situ bioremediation of fipronil contaminated soil by bioaugmentation using efficient bacterial isolates was revealed and two bacterial strains Staphylococcus arlettae and Bacillus thuringiensis could degrade fipronsil in both liquid culture and soil.
Abstract: The aim of the current study was to analyze the abundance and activity of soil microflora in response to fipronil residues, as well as conjointly to isolate and identify bacteria for the bioremedia...
11 citations
TL;DR: In this paper, the chemical and microbiological changes during spontaneous budu fermentation were elucidated on monthly basis (1–12 months) by analyzing 150 isolates, with a majority of bacteria (77%), followed by yeasts (12%) and 11% of unconfirmed species.
Abstract: The chemical and microbiological changes during spontaneous budu fermentation were elucidated on monthly basis (1–12 months). A significant increase (P budu fermentation, except for the fat content. The total microbial load decreased gradually from the initial of 6.13±0.01 to 3.45±0.13 log CFU g−1 after 12 months of fermentation. Overall, 150 isolates were identified, with a majority of bacteria (77%), followed by yeasts (12%) and 11% of unconfirmed species. Micrococcus luteus was the predominant strain that initiated the fermentation before it was replaced by Staphylococcus arlettae that exists throughout the fermentation. This study confirmed that lactic acid bacteria and yeasts often coexist with other microorganisms, even though a microbiological succession usually takes place both between and within species, which shaped the chemical and sensory characteristics of the final product. In addition, some of the isolates could be potentially valuable as starter cultures for further improved and controllable budu fermentation.
11 citations
19 Nov 2019
TL;DR: The genome of a novel methicillin resistant isolate of SAR is described, a comprehensive analysis of predicted antibiotic resistance profiles and virulence determinants for all the 22 currently available SAR genomes are presented and it is suggested that SAR is closely related to well-known pathogenic Staphylococcus species.
Abstract: Coagulase Negative Staphylococci (CoNS) are becoming increasingly recognized as an important cause of human and animal infections. Notwithstanding their clinical relevance, annotation of genes potentially involved in pathogenicity and/or antibiotic resistance in the CoNS species Staphylococcus arlettae (SAR) is currently very limited. In the current work we describe the genome of a novel methicillin resistant isolate of SAR, which we named Bari, and present a comprehensive analysis of predicted antibiotic resistance profiles and virulence determinants for all the 22 currently available SAR genomes. By comparing predicted antibiotic resistance and virulence-associated genes with those obtained from a manual selection of 148 bacterial strains belonging to 14 different species of staphylococci and to two “outgroup” species, Bacillus subtilis (BS) and Macrococcus caseoliticus (MC), we derived some interesting observations concerning the types and number of antibiotic resistance-related and virulence-like genes in SAR. Interestingly, almost 50% of the putative antibiotic resistance determinants identified in this work, which include the clinically relevant mec, van, and cls genes, were shared among all the SAR strains herein considered (Bari included). Moreover, comparison of predicted antibiotic resistance profiles suggest that SAR is closely related to well-known pathogenic Staphylococcus species, such as Staphylococcus aureus (SA) and Staphylococcus epidermidis (SE). A similar analysis of predicted virulence factors, revealed that several genes associated with pathogenesis (including, for example, ica, nuc, and ssp), which are commonly found in the genomes of pathogenic staphylococci such as Staphylococcus haemolyticus (SH) and Staphylococcus saprophyticus (SS), are observed also in the SAR strains for which a genomic sequence is available. All in all, we believe that the analyses presented in the current study, by providing a consistent and comprehensive annotation of virulence and antibiotic resistance-related genes in SAR, can constitute a valuable resource for the study of molecular mechanisms of opportunistic pathogenicity in this species.
11 citations
01 Jan 2009
TL;DR: It is demonstrated that budu is a mixed cultures fermentation involving functional strains in hydrolyzing fish protein into solubilized liquid and most of the identified strains showed weak amylolytic and pectinolytic activity.
Abstract: The study aimed to characterize the microbiological changes during fermentation of budu and hydrolytic properties of the isolated strains. The initial microbial load of the fish substrate was 5.13 ± 0.01 log CFU/g before decreased gradually to 3.20 ± 0.02 log CFU/g after 12 months of fermentation. Micrococcus sp was the predominant bacteria to initiate the fermentation before replacing by Staphylococcus sp that survived throughout the traditional process. Halophilic bacteria especially Micrococcus luteus and Staphylococcus arlettae exhibited good proteolytic and lipolytic activities compared to other isolated bacterial strains. However, most of the identified strains showed weak amylolytic and pectinolytic activity. Lactobacillus plantarum LP1, LP2, Staphylococcus arlettae SA1, Saccharomyces cerevisiae SC3 and Candida glabrata CG2 strains showed potential probiotic activities. These demonstrate that budu is a mixed cultures fermentation involving functional strains in hydrolyzing fish protein into solubilized liquid. Further study is suggested to focus on the selection of technologically important strains as starter cultures for reliable and enhanced budu fermentation.
10 citations