Topic
Staphylococcus arlettae
About: Staphylococcus arlettae is a research topic. Over the lifetime, 41 publications have been published within this topic receiving 1114 citations.
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TL;DR: By enriching with diesel fuel as a sole carbon source, two microbial consortia were obtained from different marine environment samples: one, colleted from the Huanghe Dock of Shengli Oil Field, and the other, from the Oil storage Dock Xiamen, where each isolate belongs to a novel species.
Abstract: By enriching with diesel fuel as a sole carbon source, two microbial consortia were obtained from different marine environment samples: one, colleted from the Huanghe Dock of Shengli Oil Field, and the other, from the Oil storage Dock Xiamen. Nine bacteria and one fungus were screened out from the two samples. All of the isolates show considerable biodegradability to diesel fuel except for M-5, while it shows degradability to several kinds of PAHs. Sequence analysis of 16S rDNA identifies that the 9 bacteria belong to Bacillus cereus, Pseudomonas stutzeri, Staphylococcus arlettae, Alcanivorax sp., Alcaligenes denitrificans and alpha proteobacterium (isolate M-5), respectively. Among them, each of isolates B-5 and M-5 belongs to a novel species. Two alkane monoxygenase fragments ware amplified from isolate B-5 and Pseudomonas stutzeri M-2. And the fragment retrieved from isolate B-5 seems to encode a partial novel alkane monoxygenase.
10 citations
TL;DR: The experimental coupled molecular modeling postulated a structure–activity relationship of lipase from S. arlettae JPBW‐1, a potential candidate for detergent, leather, pulp, and paper industries.
Abstract: A three-step purification of a unique lipase with halo-, solvent-, detergent-, and thermo-tolerance from Staphylococcus arlettae JPBW-1 gave raise to a 27-fold purification with a specific activity of 32.5 U/mg. The molecular weight of the purified lipase was estimated to be 45 kDa using SDS–PAGE, and its amino acid sequence was characterized using MALDI-TOF-MS analysis. The sequence obtained from MALDI-TOF-MS showed significant similarity with the capsular polysaccharide biosynthesis protein (CapD) of Staphylococcus aureus through comparative modeling approach using ROBETTA server. Identification of responsible fragments for homodimer formation was performed using comparative modeling and substrate binding domain through C-terminus matching of this new lipase with the CapD of Staphylococcus aureus was executed. Thus, the experimental coupled molecular modeling postulated a structure–activity relationship of lipase from S. arlettae JPBW-1, a potential candidate for detergent, leather, pulp, and paper industries.
7 citations
TL;DR: Oil-degrading bacteria were isolated from polluted sites in Kharg Island, which is one of the most important ports for oil export in Iran, and growth rates of these strains by its optical densities at the wavelength of 600 illustrated that Pseudomonas sp.
Abstract: Petroleum-degrading bacteria were isolated from polluted sites in Kharg Island, which is one of the most important ports for oil export in Iran. Both biochemical tests and 16S rRNA were applied to identify the strains. Experiments were performed in a mineral salt medium containing 2% of crude oil as the sole carbon. The results illustrated that the strains were: Brevibacillus sp., Microbacterium oxydans, Staphylococcus arlettae, Staphylococcus warneri, Methylobacterium persicinum, and Achromobacter xylosoxidans. Growth rates of these strains by its optical densities at the wavelength of 600 illustrated that Pseudomonas sp. (accession no. KC771232) had the highest growth rate (OD600 0.8).
6 citations
06 Apr 2016
TL;DR: The result suggested that LP1, LP2, SC3, CG2, LL2 and SA were technologically interesting and could be developed as starter cultures for the manufacturing of novel functional fermented foods.
Abstract: This study assessed the potential of probiotic and antimicrobial activity of strains isolated from an indigenous fish sauce in Malaysia. A total of 150 isolates were evaluated for their resistance toward low pH and bile salts as well as the production of inhibitory substances against four selected foodborne pathogens namely Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli O157:H7. Lactobacillus plantarum (LP1, LP2), Saccharomyces cerevisiae (SC3), Candida glabrata (CG2), Lactococcus lactis subsp. lactis (LL2) and Staphylococcus arlettae (SA) strains showed resistance to low pH and bile salt at various concentrations. The LP1(86.3%), LP2 (86.2%), LL2 (84.4%) and CG2 (79.7%) strains exhibited higher survival rates than SA (66.7%) strain at extremely low pH concentration (pH 1.5) compared to other tested strains; while most of the strains tolerate bile salt at low concentrations (0.3%) which mimic the human small intestine environment. The growth rate of the tested strains decreased in proportion to the increase of bile salt concentrations. All the strains elicited different levels of antimicrobial activities against selected pathogens. Only the LP1, SC3 and SA strains showed greater inhibitory effect against Staphylococcus aureus and Listeria monocytogenes. The result suggested that LP1, LP2, SC3, CG2, LL2 and SA were technologically interesting and could be developed as starter cultures for the manufacturing of novel functional fermented foods.
6 citations
TL;DR: The present study can facilitate in design and development of an effective anti-bac- terial compositions and dressings in veterinary medicine and animal agriculture to combat Staphylococcal infections.
Abstract: Antibiotic resistance in bacterial pathogens has become a worldwide public health problem in veteri- nary and human medicine. The emergence of methicillin resistance in Staphylococcus sp. has led to complications in treatment of bovine mastitis, wound infections in horses, dogs, pigs etc. and bacterimia in humans ranging from postules, sepsis to even death. To address this problem of antibiotic resistance, several attempts are being made of which, phage therapy offers a promising alternative. Herein, we report a phage based antibacterial nanoplatform hav- ing bactericidal potential against an isolated methicillin resistant skin bacterium, Staphylococcus arlettae as a model organism. The bacteriophages (phages) used in the study were isolated from sewage water via enrichment method against an isolated methicillin resitant skin bacterium, Staphylococcus arlettae (GenBank accession no. KF589201.1). The isolated phages were characterized thereafter for their host specificity and morphology by plaque assay and transmission electron microscopy (TEM) respectively. Intrinsic charge characteristic of phages was utilized for their immobilization on silica NPs monolyer using different conjugation chemistry, through covalent/electrostatic/ phy- sisorption interactions. The efficacy of each immobilization approach was compared using plaque assay. Silica NPs of size ~ 300 nm were synthesized by hydrolysis and condensation of tetraethylorthosilicate in an alkaline envi- ronment, and characterized using scanning electron microscopy (SEM). The particles were dispersed at air/water interface for monolayer formation on polyvinyllidine fluoride (PVDF) membrane via Langmuir-Blodgett (LB) thin film deposition technique at 15 mN/m surface pressure. It was observed that 3-aminopropyltrimethoxysilane (APTMS) treated membranes act as a better bactericidal platform due to proper orientation of phages by means of electrostatic and covalent interaction as compared to phage bound by physical adsorption. Moreoever, high sur- face/volume ratio of NPs further helped in improving bactericidal performance, associated with large loading ca- pacity of phages on film. Thus, the present study can facilitate in design and development of an effective anti-bac- terial compositions and dressings in veterinary medicine and animal agriculture to combat Staphylococcal infections.
6 citations