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Steroid metabolic process

About: Steroid metabolic process is a research topic. Over the lifetime, 40 publications have been published within this topic receiving 752 citations.

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Journal ArticleDOI
TL;DR: The presence of UGT2B gene deletion and duplication polymorphisms in Indian families is reported and network analysis indicates the substitutive role of other possible genes in the UGT activity.
Abstract: Background and Objectives. Uridine diphospho-glucuronosyltransferase 2B (UGT2B) is a family of genes involved in metabolizing steroid hormones and several other xenobiotics. These UGT2B genes are highly polymorphic in nature and have distinct polymorphisms associated with specific regions around the globe. Copy number variations (CNVs) status of UGT2B17 in Indian population is not known and their disease associations have been inconclusive. It was therefore of interest to investigate the CNV profile of UGT2B genes. Methods. We investigated the presence of CNVs in UGT2B genes in 31 members from eight Indian families using Affymetrix Genome-Wide Human SNP Array 6.0 chip. Results. Our data revealed >50% of the study members carried CNVs in UGT2B genes, of which 76% showed deletion polymorphism. CNVs were observed more in UGT2B17 (76.4%) than in UGT2B15 (17.6%). Molecular network and pathway analysis found enrichment related to steroid metabolic process, carboxylesterase activity, and sequence specific DNA binding. Interpretation and Conclusion. We report the presence of UGT2B gene deletion and duplication polymorphisms in Indian families. Network analysis indicates the substitutive role of other possible genes in the UGT activity. The CNVs of UGT2B genes are very common in individuals indicating that the effect is neutral in causing any suspected diseases.

1 citations

Journal ArticleDOI
TL;DR: In this article, the potential targets and biological mechanisms of quercetin in preventing preterm birth were clarified by using cytoHubba from the protein-protein interaction network.
Abstract: Our previous study has shown that quercetin prevented lipopolysaccharide-induced preterm birth. This study aims to clarify the potential targets and biological mechanisms of quercetin in preventing preterm birth. We used bioinformatics databases to collect the candidate targets for quercetin and preterm birth. The biological functions and enriched pathways of the intersecting targets were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Then, the hub targets were identified by cytoscape plugin cytoHubba from the protein-protein interaction network. We obtained 105 targets for quercetin in preventing preterm birth. The biological processes of the intersecting targets are mainly involved in steroid metabolic process, drug metabolic process, oxidation-reduction process, omega-hydroxylase P450 pathway, positive regulation of cell migration, negative regulation of apoptotic process, and positive regulation of cell proliferation. The highly enriched pathways were steroid hormone biosynthesis, metabolism of xenobiotics by cytochrome P450, proteoglycans in cancer, focal adhesion, and arachidonic acid metabolism. The ten hub targets for quercetin in preventing preterm birth were AKT serine/threonine kinase 1, mitogen-activated protein kinase 3, epidermal growth factor receptor, prostaglandin-endoperoxide synthase 2, mitogen-activated protein kinase 1, estrogen receptor 1, heat shock protein 90 alpha family class A member 1, mitogen-activated protein kinase 8, androgen receptor, and matrix metallopeptidase 9. Molecular docking analysis showed good bindings between these proteins and quercetin. In conclusion, these findings highlight the key targets and molecular mechanisms of quercetin in preventing preterm birth.
Book ChapterDOI
03 Aug 2019
TL;DR: A network pharmacology-based approach was adopted to provide new insights into the active compounds and therapeutic targets of Danggui-Baishao herb pair for the treatment of primary dysmenorrhea, finding that these targets were mainly involved in Steroid hormone biosynthesis, Arachidonic acid metabolism, serotonergic synapse and response to oxidative stress.
Abstract: Epidemiological studies have shown that primary dysmenorrhea is the most common disease in gynecology, and its incidence rate is between 20% and 90%, which is a common cause of affecting women’s normal work and quality of life. Its high incidence rate, wide spread and economic losses and social harm have caused widespread concern worldwide. The present work adopted a network pharmacology-based approach to provide new insights into the active compounds and therapeutic targets of Danggui-Baishao herb pair for the treatment of primary dysmenorrhea. Fifteen active compounds of the herb pair possessing favorable pharmacokinetic profiles and biological activities were selected, interacting 17 dysmenorrhea-related targets to provide potential synergistic therapeutic actions. Systematic analysis of the constructed networks revealed that these targets such as ABCB1, ESR1, PGR, AKR1C3, PTGS2, CYP2C8, PTGS1 were mainly involved in Steroid hormone biosynthesis, Arachidonic acid metabolism, serotonergic synapse and Ovarian steroidogenesis through steroid metabolic process, steroid hormone mediated signaling pathway, cyclooxygenase pathway, response to estradiol and response to oxidative stress.
01 Jan 2013
TL;DR: In pigs, conceptus attachment to the uterine surface epithelium starts around Day 14 of pregnancy preceded by a pronounced vascularization at the implantation zones, initiating the epitheliochorial placentation as discussed by the authors.
Abstract: In pigs, conceptus attachment to the uterine surface epithelium starts around Day 14 of pregnancy preceded by a pronounced vascularization at the implantation zones, initiating the epitheliochorial placentation. To characterize the complex transcriptome changes in the endometrium in the course of initial conceptus attachment, deep sequencing of endometrial RNA samples of pregnant animals (n ¼ 4) and corresponding cyclic controls (n ¼ 4) was performed using Illumina RNA-Seq. The obtained sequence reads were mapped to the porcine genome, and relative expression values were calculated for the analysis of differential gene expression. Statistical analysis revealed 1933 differentially expressed genes (false discovery rate 1%), 1229 with higher and 704 with lower mRNA concentration, in the samples from pregnant animals. Expression of selected genes was validated by the use of quantitative realtime RT-PCR. The RNA-Seq data were compared to results of a microarray study of bovine endometrium on Day 18 of pregnancy and additional related data sets. Bioinformatics analysis revealed for the genes with higher mRNA concentration in pregnant samples strong overrepresentation, particularly for immune-related functional terms but also for apoptosis and cell adhesion. Overrepresented terms for the genes with lower mRNA concentration in pregnant samples were related to extracellular region, ion transport, cell adhesion, and lipid and steroid metabolic process. In conclusion, RNA-Seq analysis revealed comprehensive transcriptome differences in porcine endometrium between Day 14 of pregnancy and corresponding cyclic endometrium and highlighted new processes and pathways probably involved in regulation of noninvasive implantation in the pig. Illumina Genome Analyzer IIx, pig, Sus scrofa, swine, uterus
Journal ArticleDOI
31 Dec 2014
TL;DR: Genes related to germ cell development, sex differentiation and defense response to bacterium were mainly expressed in the immature egg, while genes related to regulation of apoptosis, steroid metabolic process and lipid homeostasis were mainly detected in the mature egg.
Abstract: Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, KoreaABSTRACT Chicken eggs undergo various physiological changes during egg maturation. To study genes associated with the egg maturation in pre-ovulation (immature) and post-ovulation (mature), we compared gene expression patterns between in the immature egg and mature egg using RNA sequencing data. Mature and immature eggs were obtained from a Heuksaek Jaerae-jong of Korean native chicken. Total RNAs obtained from the eggs were sequenced by Illumina HiSeq 2000 platform, and the generated sequence reads were mapped to Galgal4 reference sequence assembly using Tuxedo Protocol. From the comparison of the RNA sequencing data, 315 genes were differentially expressed between mature and immature eggs, and 46 genes were only detected in immature egg. Further gene ontology (GO) analysis was performed for the differentially expressed genes using DAVID, showing that 29 and 28 GO terms were independently clustered from mature and immature, respectively. From those clustered GO terms, genes related to germ cell development, sex differentiation and defense response to bacterium were mainly expressed in the immature egg, while genes related to regulation of apoptosis, steroid metabolic process and lipid homeostasis were mainly detected in the mature egg. Our results could contribute to understand egg maturation before and after ovulation, and develop genetic markers for improving egg quality and productivity.(Key words : egg maturation, RNA sequencing, gene ontology, differential gene expression)

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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20217
20207
20194
20182
20172
20162