Topic
Sterol
About: Sterol is a research topic. Over the lifetime, 8117 publications have been published within this topic receiving 309926 citations. The topic is also known as: sterols & sterol lipids.
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TL;DR: The lipid content and composition of Chlamys tehuelcha gonads and other soft tissues were studied throughout a whole year and changes found in the lipids of the gonads may be related to the sexual cycle of the mollusc.
91 citations
TL;DR: Cholesterol sulfate as well as certain analogs can project the red blood cell against hypotonic hemolysis and is produced in vitro at physiological concentrations of the sterol sulfate and both the sulfate moiety and the side chain of the molecule are necessary for biological activity.
91 citations
TL;DR: A functional redundancy in the essential C26-monooxygenase activity of M. tuberculosis is established and two other cytochrome P450 enzymes, CYP125A1 and CYP142A1, are validated as possible drug targets.
91 citations
TL;DR: Screening of oat and Arabidopsis cDNA libraries with amplified labeled DNA fragments resulted in the isolation of sterol glucosyltransferase-specific cDNAs with insert lengths of ca.
Abstract: Steryl glucosides are characteristic lipids of plant membranes. The biosynthesis of these lipids is catalyzed by the membrane-bound UDP-glucose:sterol glucosyltransferase (EC 2.4.1.173). The purified enzyme (Warnecke and Heinz, Plant Physiol 105 (1994): 1067-1073) has been used for the cloning of a corresponding cDNA from oat (Avena sativa L.). Amino acid sequences derived from the amino terminus of the purified protein and from peptides of a trypsin digestion were used to construct oligonucleotide primers for polymerase chain reaction experiments. Screening of oat and Arabidopsis cDNA libraries with amplified labeled DNA fragments resulted in the isolation of sterol glucosyltransferase-specific cDNAs with insert lengths of ca. 2.3 kb for both plants. These cDNAs encode polypeptides of 608 (oat) and 637 (Arabidopsis) amino acid residues with molecular masses of 66 kDa and 69 kDa, respectively. The first amino acid of the purified oat protein corresponds to the amino acid 133 of the deduced polypeptide. The absence of these N-terminal amino acids reduces the molecular mass to 52 kDa, which is similar to the apparent molecular mass of 56 kDa determined for the purified protein. Different fragments of these cDNAs were expressed in Escherichia coli. Enzyme assays with homogenates of the transformed cells exhibited sterol glucosyltransferase activity.
91 citations
TL;DR: The early fetal brain appears to conserve cholesterol by keeping cholesterol 24-hydroxylase expression low until approximately E18, and new, rapid, de novo sterol synthesis in brain was confirmed by the observation that concentrations of C24,25-unsaturated sterols were low in the brains of all very young fetuses but increased rapidly beginning at approximately E11–12.
91 citations