Topic
Sterol
About: Sterol is a research topic. Over the lifetime, 8117 publications have been published within this topic receiving 309926 citations. The topic is also known as: sterols & sterol lipids.
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TL;DR: It is demonstrated that SREBPs are involved in both sterol regulation and carbohydrate activation of the FAS promoter in primary hepatocytes, and that the previously implicated Sp1 site is largely dispensable for sterolregulation in established cultured cells, whereas a CCAAT-binding factor/nuclear factor Y is critically important.
159 citations
TL;DR: Time-course experiments demonstrated that after the addition of human serum, sterol flux was rapid, with influx and efflux approaching maximum values within 8 h, and cellular sterol synthesis was reduced by 50% within 24 h.
159 citations
TL;DR: The current data suggest that the sterol regulatory elements in the HMG-CoA synthase promoter operate by a conditional positive mechanism: in the absence of sterols, regulatory proteins bind to these elements and stimulate transcription; in the presence of sterol, the regulatory proteins are inactivated and transcription decreases to the basal rate.
159 citations
TL;DR: An analogue of SPM is synthesized, 3-deoxy-2-O-stearoyl-SPM, in which an ester-linked acyl chain replaces the amide-linkedAcyl chain at C-2 and a hydrogen replaces the hydroxy group atC-3, which appears to be equally miscible in both SPM monolayers.
Abstract: To understand the structural basis for the apparent strong interaction between cholesterol and sphingomyelin (SPM), we have synthesized an analogue of SPM, 3-deoxy-2-O-stearoyl-SPM, in which an ester-linked acyl chain replaces the amide-linked acyl chain at C-2 and a hydrogen replaces the hydroxy group at C-3. We have compared the behavior of this analogue with that of 3-deoxy-N-stearoyl-SPM in monolayers and vesicles, both as pure phospholipids and in mixtures with cholesterol. The force-area isotherm of 3-deoxy-2-O-stearoyl-SPM was similar to that of 3-deoxy-N-stearoyl-SPM. The surface potential across the pure SPM monolayer at the air-water interface was larger for 3-deoxy-2-O-stearoyl-SPM than for 3-deoxy-N-stearoyl-SPM (about 430 mV and 330 mV, respectively, at 50 A2). The overall dipole moment of 3-deoxy-2-O-stearoyl-SPM was almost constant at 570 mD (between a mean molecular area range of 45-85 A2), whereas that of 3-deoxy-N-stearoyl-SPM was about 420 mD. Cholesterol appeared to be equally miscible in both SPM monolayers, as determined from the condensing effect cholesterol had on the lateral packing of the two SPMs. The oxidation of monolayer cholesterol by cholesterol oxidase was also determined using both SPMs. The stoichiometry at which free cholesterol clusters disappeared in monolayers, when going from high to low cholesterol content, was 2:1 (mol sterol/mol SPM) for both SPMs.(ABSTRACT TRUNCATED AT 250 WORDS)
158 citations
TL;DR: It is suggested that a common factor mediates the effects of sterols on the SRE-1 in all three promoters and that this factor has been functionally lost in the 25-hydroxycholesterol-resistant cells.
158 citations