Topic
Sterol
About: Sterol is a research topic. Over the lifetime, 8117 publications have been published within this topic receiving 309926 citations. The topic is also known as: sterols & sterol lipids.
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TL;DR: The results suggest that C-22 hydroxylation of CR before C-5alpha reduction is the main route of BR biosynthetic pathway, which contrasts with the generally accepted route via CN.
Abstract: Arabidopsis dwf4 is a brassinosteroid (BR)-deficient mutant, and the DWF4 gene encodes a cytochrome P450, CYP90B1. We report the catalytic activity and substrate specificity of CYP90B1. Recombinant CYP90B1 was produced in Escherichia coli, and CYP90B1 activity was measured in an in vitro assay reconstituted with NADPH-cytochrome P450 reductase. CYP90B1 converted campestanol (CN) to 6-deoxocathasterone, confirming that CYP90B1 is a steroid C-22 hydroxylase. The substrate specificity of CYP90B1 indicated that sterols with a double bond at positions C-5 and C-6 are preferred substrates compared with stanols, which have no double bond at the position. In particular, the catalytic efficiency (k[subscript cat]/K[subscript m]) of CYP90B1 for campesterol (CR) was 325 times greater than that for CN. As CR is more abundant than CN in planta, the results suggest that C-22 hydroxylation of CR before C-5[alpha] reduction is the main route of BR biosynthetic pathway, which contrasts with the generally accepted route via CN. In addition, CYP90B1 showed C-22 hydroxylation activity toward various C₂₇₋₂₉ sterols. Cholesterol (C₂₇ sterol) is the best substrate, followed by CR (C₂₈ sterol), whereas sitosterol (C₂₉ sterol) is a poor substrate, suggesting that the substrate preference of CYP90B1 may explain the discrepancy between the in planta abundance of C₂₇/C₂₈/C₂₉ sterols and C₂₇/C₂₈/C₂₉ BRs.
150 citations
TL;DR: A hitherto unreported esterified form of steryl glucoside has been isolated from potato tuber lipids and soybean phosphatides as discussed by the authors, which is composed of sterol, glucose, and fatty acid in the molar ratio 1:1:1.
149 citations
TL;DR: The findings indicate that ceramide-mediated maturation of SREBP-1 maturation is a novel sterol-independent mechanism by which cholesterol homeostasis may be regulated.
149 citations
TL;DR: The present paper shows that on a capillary column this separation is successful, so that cholesterol metabolism can be quantitated accurately without preceding TLC separation.
149 citations
TL;DR: It is demonstrated that the membrane order and occurrence of voids are determinants of membrane rigidity and hypersensitivity to a drug, and the relevance of drug permeability is considered.
149 citations