scispace - formally typeset
Search or ask a question
Topic

Strand invasion

About: Strand invasion is a research topic. Over the lifetime, 483 publications have been published within this topic receiving 26722 citations.


Papers
More filters
Journal ArticleDOI
06 Dec 1991-Science
TL;DR: The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.
Abstract: A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.

3,629 citations

Journal ArticleDOI
04 Mar 2011-Cell
TL;DR: It is proposed that the spatial and temporal control of DSB repair in heterochromatin safeguards genome stability by preventing aberrant exchanges between repeats.

480 citations

Journal ArticleDOI
TL;DR: In vitro studies indicate that PNA could inhibit both transcription and translation of genes to which it has been targeted, which holds promise for its use for antigene and antisense therapy.
Abstract: Synthetic molecules that can bind with high sequence specificity to a chosen target in a gene sequence are of major interest in medicinal and biotechnological contexts. They show promise for the development of gene therapeutic agents, diagnostic devices for genetic analysis, and as molecular tools for nucleic acid manipulations. Peptide nucleic acid (PNA) is a nucleic acid analog in which the sugar phosphate backbone of natural nucleic acid has been replaced by a synthetic peptide backbone usually formed from N-(2-amino-ethyl)-glycine units, resulting in an achiral and uncharged mimic. It is chemically stable and resistant to hydrolytic (enzymatic) cleavage and thus not expected to be degraded inside a living cell. PNA is capable of sequence-specific recognition of DNA and RNA obeying the Watson-Crick hydrogen bonding scheme, and the hybrid complexes exhibit extraordinary thermal stability and unique ionic strength effects. It may also recognize duplex homopurine sequences of DNA to which it binds by strand invasion, forming a stable PNA-DNA-PNA triplex with a looped-out DNA strand. Since its discovery, PNA has attracted major attention at the interface of chemistry and biology because of its interesting chemical, physical, and biological properties and its potential to act as an active component for diagnostic as well as pharmaceutical applications. In vitro studies indicate that PNA could inhibit both transcription and translation of genes to which it has been targeted, which holds promise for its use for antigene and antisense therapy. However, as with other high molecular mass drugs, the delivery of PNA, involving passage through the cell membrane, appears to be a general problem.

443 citations

Journal ArticleDOI
TL;DR: The mechanism of end processing is the focus of this review and the conserved MRX/MRN complex appears to be a major regulator of DNA end processing.

419 citations

Journal ArticleDOI
TL;DR: The DNA transactions and enzymatic activities required for this elegantly orchestrated process in the context of the repair of DNA double-strand breaks in somatic cells are discussed.

406 citations


Network Information
Related Topics (5)
DNA
107.1K papers, 4.7M citations
83% related
Transcription (biology)
56.5K papers, 2.9M citations
82% related
Chromatin
50.7K papers, 2.7M citations
81% related
RNA
111.6K papers, 5.4M citations
81% related
Mutant
74.5K papers, 3.4M citations
79% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202133
202020
201924
201818
201728
201623