Topic
Structural biology
About: Structural biology is a research topic. Over the lifetime, 2206 publications have been published within this topic receiving 126070 citations.
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TL;DR: By computationally reverse-engineering the duplication, fusion and diversification events in the evolutionary history of a WD40 protein, a perfectly symmetrical homologue called Tako8 was made, a fourfold-symmetrical protein in which neighbouring blades carry compensating charges.
31 citations
TL;DR: It is proposed that (partial) removal of the transcriptional silencing mechanism changes the levels of proteins essential for the functional overexpression of membrane proteins.
31 citations
TL;DR: This work has determined the structures of human MAD2L2 in complex with a CAMP fragment in two crystal forms and revealed an unprecedented mechanism involving CAMP's WK motif, which provides direct evidence for the dynamic nature of MAD2 L2 structure.
31 citations
TL;DR: This study shows that it is possible to monitor the fraction of outward-facing conformation of a wild-type ABC transporter not only under turnover conditions in vitro but also in isolated cellular membranes with no need of purification or labeling of the target transporter.
Abstract: Nanobodies are emerging tools in a variety of fields such as structural biology, cell imaging, and drug discovery. Here we pioneer the use of their spin-labeled variants as reporters of conformational dynamics of membrane proteins using DEER spectroscopy. At the example of the bacterial ABC transporter TM287/288, we show that two gadolinium-labeled nanobodies allow us to quantify, via analysis of the modulation depth of DEER traces, the fraction of transporters adopting the outward-facing state under different experimental conditions. Additionally, we quantitatively follow the interconversion from the outward- to the inward-facing state in the conformational ensemble under ATP turnover conditions. We finally show that the specificity of the nanobodies for the target protein allows the direct attainment of structural information on the wild-type TM287/288 expressed in cellular membranes without the need to purify or label the investigated membrane protein.
31 citations
TL;DR: In this article, the importance of residues outside the helix-turn-helix motif and protein conformational changes to DNA binding specificity were underscored, and the structure determination of the E. coli factor for inversion stimulation was discussed.
31 citations