Topic
Structural biology
About: Structural biology is a research topic. Over the lifetime, 2206 publications have been published within this topic receiving 126070 citations.
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TL;DR: Recent advances in studying the conformational dynamics of membrane proteins by X-ray free electron lasers by integrating sample efficient high viscosity injectors into pump probe setups are described.
25 citations
TL;DR: The AlphaFold algorithm as discussed by the authors was used to increase the number of protein structural models by almost three orders of magnitude, which is the largest increase in protein structure prediction in history.
Abstract: The release of protein structure predictions from AlphaFold will increase the number of protein structural models by almost three orders of magnitude. Structural biology and bioinformatics will never be the same, and the need for incisive experimental approaches will be greater than ever. Combining these advances in structure prediction with recent advances in cryo-electron microscopy suggests a new paradigm for structural biology.
25 citations
TL;DR: A number of specific differences in the structure of infectious and noninfectious PrP aggregates are revealed, both at the level of the polypeptide backbone and quaternary packing arrangement, to facilitate development of experimentally based high-resolution structural models of infectiosus mammalian prions.
25 citations
TL;DR: It is shown that MS unambiguously identifies the protein and lipid components of membrane protein complexes, unravels their three-dimensional arrangements and further provides clues of protein-lipid interactions.
Abstract: Membrane proteins are key players in the cell. Due to their hydrophobic nature they require solubilising agents such as detergents or membrane mimetics during purification and, consequently, are challenging targets in structural biology. In addition, their natural lipid environment is crucial for their structure and function further hampering their analysis. Alternative approaches are therefore required when the analysis by conventional techniques proves difficult. In this review, we highlight the broad application of mass spectrometry (MS) for the characterisation of membrane proteins and their interactions with lipids. We show that MS unambiguously identifies the protein and lipid components of membrane protein complexes, unravels their three-dimensional arrangements and further provides clues of protein-lipid interactions.
25 citations
TL;DR: It is anticipated that the method for ligating separate domains into a single functional multidomain protein reported here may enable many applications in structural biology.
25 citations