Topic
Structural biology
About: Structural biology is a research topic. Over the lifetime, 2206 publications have been published within this topic receiving 126070 citations.
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TL;DR: Several studies have shown that the tethering of multiple binding modules can enhance RNA-binding affinity and specificity, a strategy also commonly used in DNA recognition.
76 citations
TL;DR: Protein molecules in solution exist as an equilibrium of different conformations, but the sizes and shifts of these populations cannot be determined from static structures, so how they can be measured in solution is shown.
Abstract: Protein molecules in solution exist as an equilibrium of different conformations, but the sizes and shifts of these populations cannot be determined from static structures. A report now shows how they can be measured in solution.
76 citations
TL;DR: The methods that have been used to evaluate the thermodynamic stability of membrane proteins are reviewed and some salient examples of how the methods have beenused to begin to understand the energetics of membrane protein folding are provided.
Abstract: Learning how amino acid sequences define protein structure has been a major challenge for molecular biology since the first protein structures were determined in the 1960s. In contrast to the staggering progress with soluble proteins, investigations of membrane protein folding have long been hampered by the lack of high-resolution structures and the technical challenges associated with studying the folding process in vitro. In the past decade, however, there has been an explosion of new membrane protein structures and a slower but notable increase in efforts to study the factors that define these structures. Here we review the methods that have been used to evaluate the thermodynamic stability of membrane proteins and provide some salient examples of how the methods have been used to begin to understand the energetics of membrane protein folding.
76 citations
TL;DR: The development of data standards and a prototype system for archiving integrative/hybrid structural models of macromolecular assemblies, which provide the definitions required for representing I/H models that span multiple spatiotemporal scales and conformational states are presented.
75 citations
TL;DR: Direct measurement of the interaction of these carrier molecules with the proteins indicates that they reversibly destabilize the native state of the molecule favoring a partially unfolded conformation, which indicates these intermediate protein conformations are transport competent and are able to be absorbed through the intestinal tissue and into the bloodstream.
75 citations