Showing papers on "Strychnos nux-vomica published in 1998"
Journal Article•
TL;DR: Processing of nux vomica plays a critical role in its toxicity, and the contents of isomers and N-oxides of Strychnos alkaloids were the highest in the sand processing.
Abstract: AIM: To examine the cytotoxicities of 6 crude Strychnos alkaloid fractions from the seeds of Strychnos nux-vomica unprocessed or processed with various traditional processing methods and 13 pure Strychnos alkaloids from the fractions. METHODS: Using cell culture, their inhibitory effects on Vero cell growth-inhibition assay, and host cell DNA synthesis by [3H]thymidine ([3H]TdR) uptake assay. RESULTS: The IC50 of processed seeds were 155% and 212% of unprocessed ones in cell growth-inhibition assay and in [3H]TdR uptake assay, respectively. The IC50 of 13 compounds were 0.45-0.80 mmol.L-1 and 0.50-12 mmol.L-1, respectively. The processing method with sand bath exhibited a wide safety margin compared with other traditional processing methods or no processing. The isomers of Strychnos alkaloids and their N-oxides showed much lower cytotoxicities among these alkaloids. Isobrucine N-oxide showed the lowest cytotoxicity. The contents of isomers and N-oxides of Strychnos alkaloids were the highest in the sand processing. CONCLUSION: Processing of nux vomica plays a critical role in its toxicity.
38 citations
TL;DR: The reinvestigation of Strychnos nux-vomica resulted in the isolation of a colored monoquaternary bisindole alkaloid from the roots, and the structure of this new orange substance was defined by detailed spectroscopic methods.
Abstract: The reinvestigation of Strychnos nux-vomica resulted in the isolation of a colored monoquaternary bisindole alkaloid from the roots. The structure of this new orange substance, strychnochrysine (1), was defined by detailed spectroscopic methods.
14 citations
TL;DR: In this paper, high-speed countercurrent chromatography (CCC) was applied to the separation of strychnine and brucine from crude extract of Strychnos nux-vomica L. Using a two-phase solvent system composed of chloroform/0.07 M sodium phosphate, 0.04 M citric buffer (pH 5.08) (1:1, v/v).
Abstract: High-speed countercurrent chromatography (CCC) was applied to the separation of strychnine and brucine from crude extract of Strychnos nux-vomica L. using a two-phase solvent system composed of chloroform/0.07 M sodium phosphate, 0.04 M citric buffer (pH 5.08) (1:1, v/v). Fractionated components were identified with authentic pure compounds on TLC and also analyzed by HPLC, IR and FTMS. The results showed that from 40 g of the seed powder, 48.2 mg of strychnine was purified at 99.9% purity (83.3% recovery) while 18.1 mg of brucine fraction was obtained after rechromatographed by preparative TLC improving the purity to 91.2%.
8 citations
Journal Article•
TL;DR: The result shows that the product processed with Ephedra sinica can reduce toxicity and promote curative effect and among the different processing methods the preparation withEphedra and Liqorice root appears better and thus useful in practical application.
Abstract: Determination has been made on the contents of strychnine, brucine and ephedrin in different processed products of Strychnos nux-vomica. The acute toxicity, analgesic and antiphlogistic actions of these products have also been detected. The result shows that the product processed with Ephedra sinica can reduce toxicity and promote curative effect. Among the different processing methods the preparation with Ephedra and Liqorice root and the preparation with Ephedra and alcohol appear better and thus useful in practical application.
3 citations