Showing papers on "Strychnos nux-vomica published in 2013"
TL;DR: The study suggests that brucine potently suppresses angiogenesis by targeting VEGFR2 activation and may be a viable drug candidate in anti-angiogenesis and anti-cancer therapies.
58 citations
TL;DR: It is demonstrated that brucine dramatically repressed HepG2 and SMMC-7721 HCC cell migration with few cytotoxic effects and the inhibition of the HIF-1 pathway is implicated in the anti-metastasis activity of brucines.
44 citations
TL;DR: Three bisindolomonoterpenic alkaloids together with strychnochrysine, previously identified in the root bark of S. nux-vomica, were isolated from the stem bark and were found to display in vitro antiplasmodial activity against a chloroquine resistant strain and achloroquine sensitive strain.
19 citations
TL;DR: The anti-proliferative activity of SN-extract is associated with apoptosis, which is likely due to the presence of alkaloids, strychnine and brucine, which have been identified by MALDI-TOF-MS and RP-HPLC analysis.
Abstract: Multiple myeloma (MM) is an incurable B cell neoplasm causing significant morbidity and mortality. Despite recent advances in the MM-treatment, the disease still remains incurable; necessitating a search for new therapeutic agents. Therefore, Strychnos nux-vomica L. root extract (SN) was screened using the human MM-cell line, U266B1. SN-extract demonstrated anti-proliferative effect in a time- and dose-dependent manner. Morphological studies, cell cycle analysis by FACS indicate apoptosis. Furthermore, assessment of signaling molecules like IL-6 (interleukin-6) and CD-138 (Sydencan-1) confirmed apoptosis. The anti-proliferative activity of SN-extract is associated with apoptosis, which is likely due to the presence of alkaloids, strychnine and brucine, which have been identified by MALDI-TOF-MS and RP-HPLC analysis
16 citations
Journal Article•
TL;DR: In this paper, the processing technology parameters of Strychnos nux-vomica heated with sand were optimized in order to control quality of S.nux-veomica effectively.
Abstract: Objective:To optimize processing technology parameters of Strychnos nux-vomica heated with sand,in order to control quality of S.nux-vomica effectively.Method:With the content of brucine and strychnine as indexes,orthogonal test was used to evaluate effect of oil-sand degree,sand-material ratio,frying temperature and time on processing technology parameters of S.nux-vomica.Result:Optimum processing technology parameters were as followings:medium sand(300-600 μm),sand-material ratio 7∶ 1,frying temperature(190±5) ℃,frying time 4 min.Conclusion:Optimized processing technology was suitable and feasible,it was suitable for mass production.
1 citations
Journal Article•
TL;DR: In this article, HPLC-ESI-MS-MS was adopted to induce the characteristic fragmentation pathways of four kinds of alkaloid reference standards, which was applied to identify the alkaloids in S.nux-vomica.
Abstract: Objective:To analyze and identify the alkaloids in Strychnos nux-vomica.Method: HPLC-ESI-MS-MS was adopted to induce the characteristic fragmentation pathways of four kinds of alkaloid reference standards,which was applied to identify the alkaloids in S.nux-vomica.Result: The diversities of their structures lead to different ESI-MS-MS fragmentation.According to the fragmentation pathways,retention times,mass charge ratio and MS-MS data,eighteen alkaloids including 8 strychnine type,2 pseudo type,5 N→O type and 3 N-Me type were identified from the extract of S.nux-vomica.Conclusion: This study is helpful for the quality analysis and control of the extract of S.nux-vomica.
1 citations
TL;DR: In this article, the preparation of strychnine and brucine from a crude extract of Strychnos nuxvomica L. has been successfully performed for the first time using pH-zone- refining countercurrent chromatography.
Abstract: Preparative separation of strychnine and brucine from a crude extract of Strychnos nuxvomica L. has been successfully performed for the first time using pH-zone- refining counter-current chromatography. The experiment was performed with a two-phase solvent system composed of n-hexane–ethyl acetate–i-propanol–water (1: 3: 1.5: 4.5, v/v) where triethylamine (10 mM) was added to the upper stationary phase as a retainer and hydrochloric acid (5 mM) was added to the mobile phase as an eluter. From 1.5 g of the crude extract, 176 mg strychnine, and 480 mg brucine were obtained in a single run. The purities of these compounds were 95.1% and 97.0%, respectively, as determined by HPLC. The structures of the isolated compounds were confirmed by electron ionization-mass spectrometer (EI-MS) and 1H NMR.